Curcumin-loaded polydopamine nanoparticles-based antioxidant scaffold promote spinal cord repair though dural-regulation of macrophage polarization

Severe spinal cord injury (SCI) disrupts neuronal circulation and results in irreversible motor function loss. Treating SCI is a major challenge due to the limited regenerative capacity of axons and the inflammatory environment that hinders recovery. Macrophages play a dual role in SCI, with their p...

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Main Authors: Jianshui Mao, Liangliang Chen, Genying Zhuang, Zhiqiang Jin, Shoukun Wu, Mingshuai Ying, Lingchao Sheng, Jianwei Lv, Qiuchen Bi, Jingjia Ye
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:Materials Today Bio
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Online Access:http://www.sciencedirect.com/science/article/pii/S2590006425004557
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author Jianshui Mao
Liangliang Chen
Genying Zhuang
Zhiqiang Jin
Shoukun Wu
Mingshuai Ying
Lingchao Sheng
Jianwei Lv
Qiuchen Bi
Jingjia Ye
author_facet Jianshui Mao
Liangliang Chen
Genying Zhuang
Zhiqiang Jin
Shoukun Wu
Mingshuai Ying
Lingchao Sheng
Jianwei Lv
Qiuchen Bi
Jingjia Ye
author_sort Jianshui Mao
collection DOAJ
description Severe spinal cord injury (SCI) disrupts neuronal circulation and results in irreversible motor function loss. Treating SCI is a major challenge due to the limited regenerative capacity of axons and the inflammatory environment that hinders recovery. Macrophages play a dual role in SCI, with their polarization determining whether they exacerbate the injury or promote tissue repair. In this study, we developed a curcumin-loaded polydopamine nanoparticle antioxidant scaffold (cur-PDA@gel) as a novel therapeutic approach for SCI. This scaffold was designed to bidirectionally modulate macrophage polarization. The polydopamine-coated nanoparticles demonstrated strong antioxidant activity, effectively neutralizing reactive oxygen species (ROS) and reducing inflammation. Curcumin released from the scaffold promoted M2-type macrophage polarization while inhibiting M1-type polarization, thereby reshaping the inflammatory microenvironment at the injury site and facilitating axonal regeneration. Mechanistically, curcumin regulated macrophage polarization by suppressing the NF-κB signaling pathway and activating the JAK/STAT pathway, particularly in the context of cur-PDA@gel implantation. These findings underscore the potential of cur-PDA@gel as a promising therapeutic strategy for SCI repair by targeting macrophage polarization.
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spelling doaj-art-8d6b5f8659454c1e9742e6428ef705132025-08-20T03:48:27ZengElsevierMaterials Today Bio2590-00642025-06-013210189510.1016/j.mtbio.2025.101895Curcumin-loaded polydopamine nanoparticles-based antioxidant scaffold promote spinal cord repair though dural-regulation of macrophage polarizationJianshui Mao0Liangliang Chen1Genying Zhuang2Zhiqiang Jin3Shoukun Wu4Mingshuai Ying5Lingchao Sheng6Jianwei Lv7Qiuchen Bi8Jingjia Ye9Department of Orthopedics, The Fourth Affiliated Hospital of School of Medicine, and International School of Medicine, International Institutes of Medicine, Zhejiang University, Yiwu, Zhejiang, 322000, China; Corresponding author.College of Computer Science and Technology, Zhejiang University, Hangzhou, 310000, ChinaDepartment of Orthopedics, The Fourth Affiliated Hospital of School of Medicine, and International School of Medicine, International Institutes of Medicine, Zhejiang University, Yiwu, Zhejiang, 322000, China; College of Computer Science and Technology, Zhejiang University, Hangzhou, 310000, China; Center for Regenerative Medicine, The Fourth Affiliated Hospital of School of Medicine, and International School of Medicine, International Institutes of Medicine, Zhejiang University, Yiwu, Zhejiang, 322000, China; Department of Stomatology, The Fourth Affiliated Hospital of School of Medicine, and International School of Medicine, International Institutes of Medicine, Zhejiang University, Yiwu, Zhejiang, 322000, ChinaDepartment of Orthopedics, The Fourth Affiliated Hospital of School of Medicine, and International School of Medicine, International Institutes of Medicine, Zhejiang University, Yiwu, Zhejiang, 322000, ChinaDepartment of Orthopedics, The Fourth Affiliated Hospital of School of Medicine, and International School of Medicine, International Institutes of Medicine, Zhejiang University, Yiwu, Zhejiang, 322000, ChinaDepartment of Orthopedics, The Fourth Affiliated Hospital of School of Medicine, and International School of Medicine, International Institutes of Medicine, Zhejiang University, Yiwu, Zhejiang, 322000, ChinaDepartment of Orthopedics, The Fourth Affiliated Hospital of School of Medicine, and International School of Medicine, International Institutes of Medicine, Zhejiang University, Yiwu, Zhejiang, 322000, ChinaDepartment of Orthopedics, The Fourth Affiliated Hospital of School of Medicine, and International School of Medicine, International Institutes of Medicine, Zhejiang University, Yiwu, Zhejiang, 322000, ChinaCorresponding author.; Department of Orthopedics, The Fourth Affiliated Hospital of School of Medicine, and International School of Medicine, International Institutes of Medicine, Zhejiang University, Yiwu, Zhejiang, 322000, China; College of Computer Science and Technology, Zhejiang University, Hangzhou, 310000, China; Center for Regenerative Medicine, The Fourth Affiliated Hospital of School of Medicine, and International School of Medicine, International Institutes of Medicine, Zhejiang University, Yiwu, Zhejiang, 322000, China; Department of Stomatology, The Fourth Affiliated Hospital of School of Medicine, and International School of Medicine, International Institutes of Medicine, Zhejiang University, Yiwu, Zhejiang, 322000, ChinaCorresponding author.; Department of Orthopedics, The Fourth Affiliated Hospital of School of Medicine, and International School of Medicine, International Institutes of Medicine, Zhejiang University, Yiwu, Zhejiang, 322000, China; College of Computer Science and Technology, Zhejiang University, Hangzhou, 310000, China; Center for Regenerative Medicine, The Fourth Affiliated Hospital of School of Medicine, and International School of Medicine, International Institutes of Medicine, Zhejiang University, Yiwu, Zhejiang, 322000, China; Department of Stomatology, The Fourth Affiliated Hospital of School of Medicine, and International School of Medicine, International Institutes of Medicine, Zhejiang University, Yiwu, Zhejiang, 322000, ChinaSevere spinal cord injury (SCI) disrupts neuronal circulation and results in irreversible motor function loss. Treating SCI is a major challenge due to the limited regenerative capacity of axons and the inflammatory environment that hinders recovery. Macrophages play a dual role in SCI, with their polarization determining whether they exacerbate the injury or promote tissue repair. In this study, we developed a curcumin-loaded polydopamine nanoparticle antioxidant scaffold (cur-PDA@gel) as a novel therapeutic approach for SCI. This scaffold was designed to bidirectionally modulate macrophage polarization. The polydopamine-coated nanoparticles demonstrated strong antioxidant activity, effectively neutralizing reactive oxygen species (ROS) and reducing inflammation. Curcumin released from the scaffold promoted M2-type macrophage polarization while inhibiting M1-type polarization, thereby reshaping the inflammatory microenvironment at the injury site and facilitating axonal regeneration. Mechanistically, curcumin regulated macrophage polarization by suppressing the NF-κB signaling pathway and activating the JAK/STAT pathway, particularly in the context of cur-PDA@gel implantation. These findings underscore the potential of cur-PDA@gel as a promising therapeutic strategy for SCI repair by targeting macrophage polarization.http://www.sciencedirect.com/science/article/pii/S2590006425004557Macrophage polarizationDrug deliveryROS responseSpinal cord injuryWound healingImmune reaction
spellingShingle Jianshui Mao
Liangliang Chen
Genying Zhuang
Zhiqiang Jin
Shoukun Wu
Mingshuai Ying
Lingchao Sheng
Jianwei Lv
Qiuchen Bi
Jingjia Ye
Curcumin-loaded polydopamine nanoparticles-based antioxidant scaffold promote spinal cord repair though dural-regulation of macrophage polarization
Materials Today Bio
Macrophage polarization
Drug delivery
ROS response
Spinal cord injury
Wound healing
Immune reaction
title Curcumin-loaded polydopamine nanoparticles-based antioxidant scaffold promote spinal cord repair though dural-regulation of macrophage polarization
title_full Curcumin-loaded polydopamine nanoparticles-based antioxidant scaffold promote spinal cord repair though dural-regulation of macrophage polarization
title_fullStr Curcumin-loaded polydopamine nanoparticles-based antioxidant scaffold promote spinal cord repair though dural-regulation of macrophage polarization
title_full_unstemmed Curcumin-loaded polydopamine nanoparticles-based antioxidant scaffold promote spinal cord repair though dural-regulation of macrophage polarization
title_short Curcumin-loaded polydopamine nanoparticles-based antioxidant scaffold promote spinal cord repair though dural-regulation of macrophage polarization
title_sort curcumin loaded polydopamine nanoparticles based antioxidant scaffold promote spinal cord repair though dural regulation of macrophage polarization
topic Macrophage polarization
Drug delivery
ROS response
Spinal cord injury
Wound healing
Immune reaction
url http://www.sciencedirect.com/science/article/pii/S2590006425004557
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