Identification of novel ferroptosis-related biomarkers associated with the oxidative stress pathways in ischemic cardiomyopathy
Background: Ferroptosis is a cell death process that depends on iron and reactive oxygen species. It significantly contributes to cardiovascular diseases. However, its exact role in ischemic cardiomyopathy (ICM) is still unclear. Methods: Using bioinformatics methods, we identified new molecular tar...
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| Format: | Article |
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Elsevier
2025-02-01
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| Series: | International Journal of Cardiology: Heart & Vasculature |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352906724002501 |
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| author | Huilin Liu Yuan Xu Yuanmei Liu XueJun Han Liping Zhao Yixuan Liu Fuchun Zhang Yicheng Fu |
| author_facet | Huilin Liu Yuan Xu Yuanmei Liu XueJun Han Liping Zhao Yixuan Liu Fuchun Zhang Yicheng Fu |
| author_sort | Huilin Liu |
| collection | DOAJ |
| description | Background: Ferroptosis is a cell death process that depends on iron and reactive oxygen species. It significantly contributes to cardiovascular diseases. However, its exact role in ischemic cardiomyopathy (ICM) is still unclear. Methods: Using bioinformatics methods, we identified new molecular targets associated with ferroptosis in ICM and conducted various analyses—including correlation analysis, pathway enrichment analysis, protein interaction network construction, and analysis of transcription factor and drug interactions, to reveal the potential mechanisms behind these genes. Results: We evaluated two independent training sets of ICM, GSE57338 and GSE5406, comprising 203 ICM samples, and validation sets GSE76701 to examine differentially expressed genes (DEGs) related to ferroptosis. After extracting the intersection of the gene sets and ferroptosis-related genes, 53 DEGs were identified. Enrichment analyses showed that the alterations in ferroptosis-related DEGs were mainly enriched in oxidative stress response, and immune-related pathways. Furthermore, 11 hub genes were identified using protein–protein interaction network analysis. The key interactions between 11 hub genes were more pronounced in protein localization during ICM development. In addition, we construct a hub gene and transcription factor interaction network and a small molecule drug-gene interaction network. We found that among these hub genes, the N-acetylneuraminate outer membrane channel(NANC) gene is positively correlated with most of the small-molecule drugs used to treat ICM, and its high expression might increase resistance. Conclusions: Ferroptosis exists in ICM and and is associated with oxidative stress. This association suggests that ferroptosis may facilitate the progression of ICM. |
| format | Article |
| id | doaj-art-8d6a2a5842ee46d68dbf1c953a9c0132 |
| institution | DOAJ |
| issn | 2352-9067 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Journal of Cardiology: Heart & Vasculature |
| spelling | doaj-art-8d6a2a5842ee46d68dbf1c953a9c01322025-08-20T03:11:06ZengElsevierInternational Journal of Cardiology: Heart & Vasculature2352-90672025-02-015610158410.1016/j.ijcha.2024.101584Identification of novel ferroptosis-related biomarkers associated with the oxidative stress pathways in ischemic cardiomyopathyHuilin Liu0Yuan Xu1Yuanmei Liu2XueJun Han3Liping Zhao4Yixuan Liu5Fuchun Zhang6Yicheng Fu7Department of Geriatrics, Peking University Third Hospital, Beijing 100191, PR ChinaDepartment of Pathology, Zhongshan Hospital, Fudan University, Shanghai 200032, PR ChinaDepartment of Geriatrics, Peking University Third Hospital, Beijing 100191, PR ChinaDepartment of Orthopaedics, Jiayuguan Municipal First People’s Hospital, Jiayuguan 735100, PR ChinaDepartment of Ophthalmology, Jiayuguan Municipal First People’s Hospital, Jiayuguan 735100, PR ChinaCollege of Basic Medical Sciences, Fujian Medical University, Fuzhou 350122, PR ChinaDepartment of Geriatrics, Peking University Third Hospital, Beijing 100191, PR ChinaDepartment of Geriatrics, Peking University Third Hospital, Beijing 100191, PR China; Corresponding author at: Department of Geriatrics, Peking University Third Hospital, No.49 Huayuanbei Road, Haidian Distrct, Beijing 100191, PR China.Background: Ferroptosis is a cell death process that depends on iron and reactive oxygen species. It significantly contributes to cardiovascular diseases. However, its exact role in ischemic cardiomyopathy (ICM) is still unclear. Methods: Using bioinformatics methods, we identified new molecular targets associated with ferroptosis in ICM and conducted various analyses—including correlation analysis, pathway enrichment analysis, protein interaction network construction, and analysis of transcription factor and drug interactions, to reveal the potential mechanisms behind these genes. Results: We evaluated two independent training sets of ICM, GSE57338 and GSE5406, comprising 203 ICM samples, and validation sets GSE76701 to examine differentially expressed genes (DEGs) related to ferroptosis. After extracting the intersection of the gene sets and ferroptosis-related genes, 53 DEGs were identified. Enrichment analyses showed that the alterations in ferroptosis-related DEGs were mainly enriched in oxidative stress response, and immune-related pathways. Furthermore, 11 hub genes were identified using protein–protein interaction network analysis. The key interactions between 11 hub genes were more pronounced in protein localization during ICM development. In addition, we construct a hub gene and transcription factor interaction network and a small molecule drug-gene interaction network. We found that among these hub genes, the N-acetylneuraminate outer membrane channel(NANC) gene is positively correlated with most of the small-molecule drugs used to treat ICM, and its high expression might increase resistance. Conclusions: Ferroptosis exists in ICM and and is associated with oxidative stress. This association suggests that ferroptosis may facilitate the progression of ICM.http://www.sciencedirect.com/science/article/pii/S2352906724002501FerroptosisOxidative stressIschemic cardiomyopathyBioinformaticsDifferentially expressed genes |
| spellingShingle | Huilin Liu Yuan Xu Yuanmei Liu XueJun Han Liping Zhao Yixuan Liu Fuchun Zhang Yicheng Fu Identification of novel ferroptosis-related biomarkers associated with the oxidative stress pathways in ischemic cardiomyopathy International Journal of Cardiology: Heart & Vasculature Ferroptosis Oxidative stress Ischemic cardiomyopathy Bioinformatics Differentially expressed genes |
| title | Identification of novel ferroptosis-related biomarkers associated with the oxidative stress pathways in ischemic cardiomyopathy |
| title_full | Identification of novel ferroptosis-related biomarkers associated with the oxidative stress pathways in ischemic cardiomyopathy |
| title_fullStr | Identification of novel ferroptosis-related biomarkers associated with the oxidative stress pathways in ischemic cardiomyopathy |
| title_full_unstemmed | Identification of novel ferroptosis-related biomarkers associated with the oxidative stress pathways in ischemic cardiomyopathy |
| title_short | Identification of novel ferroptosis-related biomarkers associated with the oxidative stress pathways in ischemic cardiomyopathy |
| title_sort | identification of novel ferroptosis related biomarkers associated with the oxidative stress pathways in ischemic cardiomyopathy |
| topic | Ferroptosis Oxidative stress Ischemic cardiomyopathy Bioinformatics Differentially expressed genes |
| url | http://www.sciencedirect.com/science/article/pii/S2352906724002501 |
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