CXCL14 deficiency does not impact the outcome of influenza or Escherichia coli infections in mice
Introduction: Chemokines are small proteins that regulate different cellular functions, such as leukocyte activation, chemoattraction and inflammation. The chemokine CXCL14 (BRAK) is a highly conserved gene among species and through evolution. It has been shown that CXCL14 is locally upregulated dur...
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| Format: | Article |
| Language: | English |
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The Journal of Infection in Developing Countries
2014-10-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/3890 |
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| author | Abubaker ME Sidahmed Alberto J Leon David Banner Alyson A Kelvin Thomas Rowe Ivo Boudakov Norbert Degousse Barry B Rubin David J Kelvin |
| author_facet | Abubaker ME Sidahmed Alberto J Leon David Banner Alyson A Kelvin Thomas Rowe Ivo Boudakov Norbert Degousse Barry B Rubin David J Kelvin |
| author_sort | Abubaker ME Sidahmed |
| collection | DOAJ |
| description | Introduction: Chemokines are small proteins that regulate different cellular functions, such as leukocyte activation, chemoattraction and inflammation. The chemokine CXCL14 (BRAK) is a highly conserved gene among species and through evolution. It has been shown that CXCL14 is locally upregulated during viral infections, also, it has been found that this chemokine possesses direct antibacterial activities. Nonetheless, the exact role that CXCL14 plays during infection remains elusive.
Methodology: CXCL14 deficient mice were generated in a C57B6/129 background and followed by phenotypic characterization. Later, the effect of CXCL14 deficiency during influenza infection and E. coli challenge was assessed.
Results: Other than a slight weight reduction, CXCL14 deficient mice exhibited no phenotypic alterations. CXCL14 deficiency did not influence the outcome of influenza virus infection or challenge with E. coli, and no statistically significant differences in clinical signs, cellular responses and histopathological findings were observed.
Conclusions: CXCL14 does not seem to play a pivotal role during influenza and E. coli infections of the lung; these results are suggestive of functional overlap between CXCL14 and other chemokines that are present during lung infection.
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| format | Article |
| id | doaj-art-8d576a8a03eb4f1397d96358891a0cb7 |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2014-10-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-8d576a8a03eb4f1397d96358891a0cb72025-08-20T02:27:18ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802014-10-0181010.3855/jidc.3890CXCL14 deficiency does not impact the outcome of influenza or Escherichia coli infections in miceAbubaker ME Sidahmed0Alberto J Leon1David Banner2Alyson A Kelvin3Thomas Rowe4Ivo Boudakov5Norbert Degousse6Barry B Rubin7David J Kelvin8Toronto General Research Institute, University Health Network, Toronto, Ontario, CanadaInstitute of Medical Science, University of Toronto, Toronto, Ontario, CanadaToronto General Research Institute, University Health Network, Toronto, Ontario, CanadaImmune Diagnostics and Research, Toronto, Ontario, CanadaToronto General Research Institute, University Health Network, Toronto, Ontario, CanadaToronto General Research Institute, University Health Network, Toronto, Ontario, CanadaToronto General Hospital, University Health Network, Toronto, Ontario, CanadaToronto General Hospital, University Health Network, Toronto, Ontario, CanadaToronto General Research Institute, University Health Network, Toronto, Ontario, CanadaIntroduction: Chemokines are small proteins that regulate different cellular functions, such as leukocyte activation, chemoattraction and inflammation. The chemokine CXCL14 (BRAK) is a highly conserved gene among species and through evolution. It has been shown that CXCL14 is locally upregulated during viral infections, also, it has been found that this chemokine possesses direct antibacterial activities. Nonetheless, the exact role that CXCL14 plays during infection remains elusive. Methodology: CXCL14 deficient mice were generated in a C57B6/129 background and followed by phenotypic characterization. Later, the effect of CXCL14 deficiency during influenza infection and E. coli challenge was assessed. Results: Other than a slight weight reduction, CXCL14 deficient mice exhibited no phenotypic alterations. CXCL14 deficiency did not influence the outcome of influenza virus infection or challenge with E. coli, and no statistically significant differences in clinical signs, cellular responses and histopathological findings were observed. Conclusions: CXCL14 does not seem to play a pivotal role during influenza and E. coli infections of the lung; these results are suggestive of functional overlap between CXCL14 and other chemokines that are present during lung infection. https://jidc.org/index.php/journal/article/view/3890CXCL14BRAKpneumoniainfluenza |
| spellingShingle | Abubaker ME Sidahmed Alberto J Leon David Banner Alyson A Kelvin Thomas Rowe Ivo Boudakov Norbert Degousse Barry B Rubin David J Kelvin CXCL14 deficiency does not impact the outcome of influenza or Escherichia coli infections in mice Journal of Infection in Developing Countries CXCL14 BRAK pneumonia influenza |
| title | CXCL14 deficiency does not impact the outcome of influenza or Escherichia coli infections in mice |
| title_full | CXCL14 deficiency does not impact the outcome of influenza or Escherichia coli infections in mice |
| title_fullStr | CXCL14 deficiency does not impact the outcome of influenza or Escherichia coli infections in mice |
| title_full_unstemmed | CXCL14 deficiency does not impact the outcome of influenza or Escherichia coli infections in mice |
| title_short | CXCL14 deficiency does not impact the outcome of influenza or Escherichia coli infections in mice |
| title_sort | cxcl14 deficiency does not impact the outcome of influenza or escherichia coli infections in mice |
| topic | CXCL14 BRAK pneumonia influenza |
| url | https://jidc.org/index.php/journal/article/view/3890 |
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