NAC Transcription Factor <i>GmNAC035</i> Exerts a Positive Regulatory Role in Enhancing Salt Stress Tolerance in Plants

Soybean, a globally significant and versatile crop, serves as a vital source of both oil and protein. However, environmental factors such as soil salinization pose substantial challenges to its cultivation, adversely affecting both yield and quality. Enhancing the salt tolerance of soybeans can miti...

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Main Authors: Wanting Shi, Sixin Ye, Yiting Xin, Hongmiao Jin, Meiling Hu, Yueping Zheng, Yihua Zhan, Hongbo Liu, Yi Gan, Zhifu Zheng, Tian Pan
Format: Article
Language:English
Published: MDPI AG 2025-05-01
Series:Plants
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Online Access:https://www.mdpi.com/2223-7747/14/9/1391
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Summary:Soybean, a globally significant and versatile crop, serves as a vital source of both oil and protein. However, environmental factors such as soil salinization pose substantial challenges to its cultivation, adversely affecting both yield and quality. Enhancing the salt tolerance of soybeans can mitigate yield losses and promote the development of the soybean industry. Members of the plant-specific transcription factor family NAC play crucial roles in plant adaptation to abiotic stress conditions. In this study, we screened the soybean <i>GmNAC</i> family genes potentially involved in the salt stress response and identified 18 <i>GmNAC</i> genes that may function during the early stages of salt stress. Among these, the <i>GmNAC035</i> gene exhibited a rapid increase in expression within one hour of salt treatment, with its expression being induced by abscisic acid (ABA) and methyl jasmonate (MeJA), suggesting its significant role in the soybean salt stress response. We further elucidated the role of <i>GmNAC035</i> in soybean salt tolerance. GmNAC035, a nuclear-localized transcriptional activator, enhances salt tolerance when overexpressed in Arabidopsis, reducing oxidative damage and boosting the expression of stress-responsive genes. It achieves this by regulating key stress response pathways, including the SOS pathway, calcium signaling, and ABA signaling. These findings highlight the potential of <i>GmNAC035</i> as a genetic engineering target to improve crop salt tolerance.
ISSN:2223-7747