Characterization of a New Immunosuppressive and Antimicrobial Peptide, DRS-DA2, Isolated from the Mexican Frog, Pachymedusa dacnicolor
Inflammatory and antimicrobial diseases constitute a major burden for society, and fighting them is a WHO strategic priority. Most of the treatments available to fight inflammatory diseases are anti-inflammatory drugs, such as corticosteroids or immunomodulators that lack cellular specificity and le...
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| Format: | Article |
| Language: | English |
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Wiley
2024-01-01
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| Series: | International Journal of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2024/2205864 |
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| author | Claire Lacombe Estefania Aleman-Navaro Thierry Drujon Veronica Martinez-Osorio Emmanuelle Sachon Erika Melchy-Pérez Ludovic Carlier Lorena Elizabeth Fajardo Brigido Yannick Fleury Christophe Piesse Guadalupe Gutiérrez-Escobedo Alejandro De las Peñas Irene Castaño Florie Desriac Jose Luis Beristain-Hernandez Christophe Combadiere Yvonne Rosenstein Constance Auvynet |
| author_facet | Claire Lacombe Estefania Aleman-Navaro Thierry Drujon Veronica Martinez-Osorio Emmanuelle Sachon Erika Melchy-Pérez Ludovic Carlier Lorena Elizabeth Fajardo Brigido Yannick Fleury Christophe Piesse Guadalupe Gutiérrez-Escobedo Alejandro De las Peñas Irene Castaño Florie Desriac Jose Luis Beristain-Hernandez Christophe Combadiere Yvonne Rosenstein Constance Auvynet |
| author_sort | Claire Lacombe |
| collection | DOAJ |
| description | Inflammatory and antimicrobial diseases constitute a major burden for society, and fighting them is a WHO strategic priority. Most of the treatments available to fight inflammatory diseases are anti-inflammatory drugs, such as corticosteroids or immunomodulators that lack cellular specificity and lead to numerous side effects. In addition to suppressing undesired inflammation and reducing disease progression, these drugs lessen the immune system protective functions. Furthermore, treating infectious diseases is more and more challenging due to the rise of microbial resistance to antimicrobial drugs. Thus, controlling the inflammatory process locally without compromising the ability to combat infections is an essential feature in the treatment of inflammatory diseases. We isolated three forms (DRS-DA2N, DRS-DA2NE, and DRS-DA2NEQ) of the same peptide, DRS-DA2, which belongs to the dermaseptin family, from the Mexican tree frog Pachymedusa dacnicolor. Interestingly, DRS-DA2N and DRS-DA2NEQ exhibit a dual activity by inducing the death of leukocytes as well as that of Gram-negative and Gram-positive bacteria, including multiresistant strains, without affecting other cells such as epithelial cells or erythrocytes. We showed that the death of both immune cells and bacteria is induced rapidly by DRS-DA2 and that the membrane is permeabilized, leading to the loss of membrane integrity. We also validated the capacity of DRS-DA2 to regulate the pool of inflammatory cells in vivo in a mouse model of noninfectious peritonitis. After the induction of peritonitis, a local injection of DRS-DA2N could decrease the number of inflammatory cells locally in the peritoneal cavity without inducing a systemic effect, as no changes in the number of inflammatory cells could be detected in blood or in the bone marrow. Collectively, these data suggest that this peptide could be a promising tool in the treatment of inflammatory diseases, such as inflammatory skin diseases, as it could reduce the number of inflammatory cells locally without suppressing the ability to combat infections. |
| format | Article |
| id | doaj-art-8d3adf6a2c4a4aba94f202f523bb8b04 |
| institution | Kabale University |
| issn | 2042-0099 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Inflammation |
| spelling | doaj-art-8d3adf6a2c4a4aba94f202f523bb8b042025-02-03T06:47:12ZengWileyInternational Journal of Inflammation2042-00992024-01-01202410.1155/2024/2205864Characterization of a New Immunosuppressive and Antimicrobial Peptide, DRS-DA2, Isolated from the Mexican Frog, Pachymedusa dacnicolorClaire Lacombe0Estefania Aleman-Navaro1Thierry Drujon2Veronica Martinez-Osorio3Emmanuelle Sachon4Erika Melchy-Pérez5Ludovic Carlier6Lorena Elizabeth Fajardo Brigido7Yannick Fleury8Christophe Piesse9Guadalupe Gutiérrez-Escobedo10Alejandro De las Peñas11Irene Castaño12Florie Desriac13Jose Luis Beristain-Hernandez14Christophe Combadiere15Yvonne Rosenstein16Constance Auvynet17Laboratoire des BiomoléculesInstituto de BiotecnologíaLaboratoire des BiomoléculesInstituto de BiotecnologíaLaboratoire des BiomoléculesInstituto de BiotecnologíaLaboratoire des BiomoléculesInstituto de BiotecnologíaLUBEM EA 3882Plateforme de Synthèse PeptidiqueIPICYTIPICYTIPICYTLUBEM EA 3882Hepatobiliary and Pancreatic Surgery ClinicSorbonne UniversitéInstituto de BiotecnologíaInstituto de BiotecnologíaInflammatory and antimicrobial diseases constitute a major burden for society, and fighting them is a WHO strategic priority. Most of the treatments available to fight inflammatory diseases are anti-inflammatory drugs, such as corticosteroids or immunomodulators that lack cellular specificity and lead to numerous side effects. In addition to suppressing undesired inflammation and reducing disease progression, these drugs lessen the immune system protective functions. Furthermore, treating infectious diseases is more and more challenging due to the rise of microbial resistance to antimicrobial drugs. Thus, controlling the inflammatory process locally without compromising the ability to combat infections is an essential feature in the treatment of inflammatory diseases. We isolated three forms (DRS-DA2N, DRS-DA2NE, and DRS-DA2NEQ) of the same peptide, DRS-DA2, which belongs to the dermaseptin family, from the Mexican tree frog Pachymedusa dacnicolor. Interestingly, DRS-DA2N and DRS-DA2NEQ exhibit a dual activity by inducing the death of leukocytes as well as that of Gram-negative and Gram-positive bacteria, including multiresistant strains, without affecting other cells such as epithelial cells or erythrocytes. We showed that the death of both immune cells and bacteria is induced rapidly by DRS-DA2 and that the membrane is permeabilized, leading to the loss of membrane integrity. We also validated the capacity of DRS-DA2 to regulate the pool of inflammatory cells in vivo in a mouse model of noninfectious peritonitis. After the induction of peritonitis, a local injection of DRS-DA2N could decrease the number of inflammatory cells locally in the peritoneal cavity without inducing a systemic effect, as no changes in the number of inflammatory cells could be detected in blood or in the bone marrow. Collectively, these data suggest that this peptide could be a promising tool in the treatment of inflammatory diseases, such as inflammatory skin diseases, as it could reduce the number of inflammatory cells locally without suppressing the ability to combat infections.http://dx.doi.org/10.1155/2024/2205864 |
| spellingShingle | Claire Lacombe Estefania Aleman-Navaro Thierry Drujon Veronica Martinez-Osorio Emmanuelle Sachon Erika Melchy-Pérez Ludovic Carlier Lorena Elizabeth Fajardo Brigido Yannick Fleury Christophe Piesse Guadalupe Gutiérrez-Escobedo Alejandro De las Peñas Irene Castaño Florie Desriac Jose Luis Beristain-Hernandez Christophe Combadiere Yvonne Rosenstein Constance Auvynet Characterization of a New Immunosuppressive and Antimicrobial Peptide, DRS-DA2, Isolated from the Mexican Frog, Pachymedusa dacnicolor International Journal of Inflammation |
| title | Characterization of a New Immunosuppressive and Antimicrobial Peptide, DRS-DA2, Isolated from the Mexican Frog, Pachymedusa dacnicolor |
| title_full | Characterization of a New Immunosuppressive and Antimicrobial Peptide, DRS-DA2, Isolated from the Mexican Frog, Pachymedusa dacnicolor |
| title_fullStr | Characterization of a New Immunosuppressive and Antimicrobial Peptide, DRS-DA2, Isolated from the Mexican Frog, Pachymedusa dacnicolor |
| title_full_unstemmed | Characterization of a New Immunosuppressive and Antimicrobial Peptide, DRS-DA2, Isolated from the Mexican Frog, Pachymedusa dacnicolor |
| title_short | Characterization of a New Immunosuppressive and Antimicrobial Peptide, DRS-DA2, Isolated from the Mexican Frog, Pachymedusa dacnicolor |
| title_sort | characterization of a new immunosuppressive and antimicrobial peptide drs da2 isolated from the mexican frog pachymedusa dacnicolor |
| url | http://dx.doi.org/10.1155/2024/2205864 |
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