The Effect of CDKN1A on the Expression of Genes Related to Milk Protein and Milk Fat Synthesis in Bovine Mammary Epithelial Cells
Milk fat is an important nutritional component and flavor substance in dairy products. Its content and composition directly affect the nutritional value, processing characteristics, and economic benefits of dairy products. Therefore, an in-depth exploration of the molecular mechanisms that influence...
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2025-06-01
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| Series: | Veterinary Sciences |
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| author | Yuanyuan Zhang Junxi Liang Kai Zhang Hong Su Daqing Wang Min Zhang Feifei Zhao Zhiwei Sun Zhimin Wu Guifang Cao Yong Zhang |
| author_facet | Yuanyuan Zhang Junxi Liang Kai Zhang Hong Su Daqing Wang Min Zhang Feifei Zhao Zhiwei Sun Zhimin Wu Guifang Cao Yong Zhang |
| author_sort | Yuanyuan Zhang |
| collection | DOAJ |
| description | Milk fat is an important nutritional component and flavor substance in dairy products. Its content and composition directly affect the nutritional value, processing characteristics, and economic benefits of dairy products. Therefore, an in-depth exploration of the molecular mechanisms that influence milk protein synthesis holds profound significance for dairy farming and dairy production. Molecular biology techniques were used to construct CDKN1A overexpression and interference vectors. Using BMECs (bovine mammary epithelial cells) as the experimental model, the vectors were transfected into the cells via liposome mediation to investigate the effect of CDKN1A on the expression of genes related to milk protein synthesis. The results showed that the CDKN1A overexpression and interference vectors were successfully constructed, and the overexpression of CDKN1A reduced milk protein synthesis, and the interference of CDKN1A enhanced milk protein synthesis. This finding provides an important theoretical basis for dairy farming and dairy production. By regulating the expression level of CDKN1A, it is possible to achieve precise control of milk protein yield in dairy cows. It also offers a potential target for the development of new feed additives or drugs. These additives or drugs can promote milk protein synthesis by regulating the activity of CDKN1A, providing new strategies and methods for the sustainable development of the dairy industry. |
| format | Article |
| id | doaj-art-8d36308d81a54aa69a2a6f861de333be |
| institution | Kabale University |
| issn | 2306-7381 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj-art-8d36308d81a54aa69a2a6f861de333be2025-08-20T03:32:31ZengMDPI AGVeterinary Sciences2306-73812025-06-0112653410.3390/vetsci12060534The Effect of CDKN1A on the Expression of Genes Related to Milk Protein and Milk Fat Synthesis in Bovine Mammary Epithelial CellsYuanyuan Zhang0Junxi Liang1Kai Zhang2Hong Su3Daqing Wang4Min Zhang5Feifei Zhao6Zhiwei Sun7Zhimin Wu8Guifang Cao9Yong Zhang10College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, ChinaTongliao Institute of Agricultural and Animal Husbandry Sciences, Tongliao 028000, ChinaCollege of Life Sciences, Inner Mongolia University, Hohhot 010021, ChinaTongliao Institute of Agricultural and Animal Husbandry Sciences, Tongliao 028000, ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, ChinaMilk fat is an important nutritional component and flavor substance in dairy products. Its content and composition directly affect the nutritional value, processing characteristics, and economic benefits of dairy products. Therefore, an in-depth exploration of the molecular mechanisms that influence milk protein synthesis holds profound significance for dairy farming and dairy production. Molecular biology techniques were used to construct CDKN1A overexpression and interference vectors. Using BMECs (bovine mammary epithelial cells) as the experimental model, the vectors were transfected into the cells via liposome mediation to investigate the effect of CDKN1A on the expression of genes related to milk protein synthesis. The results showed that the CDKN1A overexpression and interference vectors were successfully constructed, and the overexpression of CDKN1A reduced milk protein synthesis, and the interference of CDKN1A enhanced milk protein synthesis. This finding provides an important theoretical basis for dairy farming and dairy production. By regulating the expression level of CDKN1A, it is possible to achieve precise control of milk protein yield in dairy cows. It also offers a potential target for the development of new feed additives or drugs. These additives or drugs can promote milk protein synthesis by regulating the activity of CDKN1A, providing new strategies and methods for the sustainable development of the dairy industry.https://www.mdpi.com/2306-7381/12/6/534bovine mammary epithelial cellsCDKN1Amilk protein synthesismilk fat synthesisexpression vector construction |
| spellingShingle | Yuanyuan Zhang Junxi Liang Kai Zhang Hong Su Daqing Wang Min Zhang Feifei Zhao Zhiwei Sun Zhimin Wu Guifang Cao Yong Zhang The Effect of CDKN1A on the Expression of Genes Related to Milk Protein and Milk Fat Synthesis in Bovine Mammary Epithelial Cells Veterinary Sciences bovine mammary epithelial cells CDKN1A milk protein synthesis milk fat synthesis expression vector construction |
| title | The Effect of CDKN1A on the Expression of Genes Related to Milk Protein and Milk Fat Synthesis in Bovine Mammary Epithelial Cells |
| title_full | The Effect of CDKN1A on the Expression of Genes Related to Milk Protein and Milk Fat Synthesis in Bovine Mammary Epithelial Cells |
| title_fullStr | The Effect of CDKN1A on the Expression of Genes Related to Milk Protein and Milk Fat Synthesis in Bovine Mammary Epithelial Cells |
| title_full_unstemmed | The Effect of CDKN1A on the Expression of Genes Related to Milk Protein and Milk Fat Synthesis in Bovine Mammary Epithelial Cells |
| title_short | The Effect of CDKN1A on the Expression of Genes Related to Milk Protein and Milk Fat Synthesis in Bovine Mammary Epithelial Cells |
| title_sort | effect of cdkn1a on the expression of genes related to milk protein and milk fat synthesis in bovine mammary epithelial cells |
| topic | bovine mammary epithelial cells CDKN1A milk protein synthesis milk fat synthesis expression vector construction |
| url | https://www.mdpi.com/2306-7381/12/6/534 |
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