Detection of hibiscus chlorotic ringspot virus, citrus exocortis viroid and citrus viroid VI in China rose from Italy using high-throughput sequencing
In Spring 2024, a symptomatic plant of China rose (Hibiscus rosa-sinensis) showing leaf yellowing and deformation, located in the province of Naples (South Italy), was re-sampled for further investigations. Total RNAs were purified from leaves and subjected to Illumina HTS analysis. Results confirme...
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| Format: | Article |
| Language: | English |
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Firenze University Press
2024-09-01
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| Series: | Phytopathologia Mediterranea |
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| Online Access: | https://oajournals.fupress.net/index.php/pm/article/view/15435 |
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| author | Giuseppe PARRELLA Chantal FAURE Armelle MARAIS Elisa TROIANO Thierry CANDRESSE |
| author_facet | Giuseppe PARRELLA Chantal FAURE Armelle MARAIS Elisa TROIANO Thierry CANDRESSE |
| author_sort | Giuseppe PARRELLA |
| collection | DOAJ |
| description | In Spring 2024, a symptomatic plant of China rose (Hibiscus rosa-sinensis) showing leaf yellowing and deformation, located in the province of Naples (South Italy), was re-sampled for further investigations. Total RNAs were purified from leaves and subjected to Illumina HTS analysis. Results confirmed that the plant was infected by hibiscus chlorotic ringspot virus (HCRSV), citrus exocortis viroid (CEVd), and citrus viroid VI (CVd-VI). The two viroids had not been previously detected in China rose. HTS results were confirmed by RT-PCR in the re-sampled plant, and in three nearby China rose plants with the same symptoms, using specific primer pairs for the three pathogens, followed by Sanger sequencing and BLAST analysis of the sequences. Different results obtained can be due to differing sensitivity and specificity in HTS-based detection of plant viruses. This is the first report of multiple infections by HCRS, CEVd and CVd-VI in China rose, which is also a new host for both of the viroids. |
| format | Article |
| id | doaj-art-8d310d1490da4fbcaae312a441e11872 |
| institution | DOAJ |
| issn | 0031-9465 1593-2095 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | Firenze University Press |
| record_format | Article |
| series | Phytopathologia Mediterranea |
| spelling | doaj-art-8d310d1490da4fbcaae312a441e118722025-08-20T03:05:25ZengFirenze University PressPhytopathologia Mediterranea0031-94651593-20952024-09-0163225525710.36253/phyto-1543514272Detection of hibiscus chlorotic ringspot virus, citrus exocortis viroid and citrus viroid VI in China rose from Italy using high-throughput sequencingGiuseppe PARRELLA0https://orcid.org/0000-0002-0412-4014Chantal FAURE1Armelle MARAIS2https://orcid.org/0000-0003-2482-1543Elisa TROIANO3https://orcid.org/0000-0001-7755-4915Thierry CANDRESSE4https://orcid.org/0000-0001-9757-1835Institute for Sustainable Plant Protection of National Research Council (IPSP-CNR), 80055 PorticiUniversity of Bordeaux, INRAE, UMR BFP, CS 20032, 33882 Villenave d’Ornon CEDEXUniversity of Bordeaux, INRAE, UMR BFP, CS 20032, 33882 Villenave d’Ornon CEDEXInstitute for Sustainable Plant Protection of National Research Council (IPSP-CNR), 80055 PorticiUniversity of Bordeaux, INRAE, UMR BFP, CS 20032, 33882 Villenave d’Ornon CEDEXIn Spring 2024, a symptomatic plant of China rose (Hibiscus rosa-sinensis) showing leaf yellowing and deformation, located in the province of Naples (South Italy), was re-sampled for further investigations. Total RNAs were purified from leaves and subjected to Illumina HTS analysis. Results confirmed that the plant was infected by hibiscus chlorotic ringspot virus (HCRSV), citrus exocortis viroid (CEVd), and citrus viroid VI (CVd-VI). The two viroids had not been previously detected in China rose. HTS results were confirmed by RT-PCR in the re-sampled plant, and in three nearby China rose plants with the same symptoms, using specific primer pairs for the three pathogens, followed by Sanger sequencing and BLAST analysis of the sequences. Different results obtained can be due to differing sensitivity and specificity in HTS-based detection of plant viruses. This is the first report of multiple infections by HCRS, CEVd and CVd-VI in China rose, which is also a new host for both of the viroids.https://oajournals.fupress.net/index.php/pm/article/view/15435hcrsvcevdcvd-vimixed infectionsngs |
| spellingShingle | Giuseppe PARRELLA Chantal FAURE Armelle MARAIS Elisa TROIANO Thierry CANDRESSE Detection of hibiscus chlorotic ringspot virus, citrus exocortis viroid and citrus viroid VI in China rose from Italy using high-throughput sequencing Phytopathologia Mediterranea hcrsv cevd cvd-vi mixed infections ngs |
| title | Detection of hibiscus chlorotic ringspot virus, citrus exocortis viroid and citrus viroid VI in China rose from Italy using high-throughput sequencing |
| title_full | Detection of hibiscus chlorotic ringspot virus, citrus exocortis viroid and citrus viroid VI in China rose from Italy using high-throughput sequencing |
| title_fullStr | Detection of hibiscus chlorotic ringspot virus, citrus exocortis viroid and citrus viroid VI in China rose from Italy using high-throughput sequencing |
| title_full_unstemmed | Detection of hibiscus chlorotic ringspot virus, citrus exocortis viroid and citrus viroid VI in China rose from Italy using high-throughput sequencing |
| title_short | Detection of hibiscus chlorotic ringspot virus, citrus exocortis viroid and citrus viroid VI in China rose from Italy using high-throughput sequencing |
| title_sort | detection of hibiscus chlorotic ringspot virus citrus exocortis viroid and citrus viroid vi in china rose from italy using high throughput sequencing |
| topic | hcrsv cevd cvd-vi mixed infections ngs |
| url | https://oajournals.fupress.net/index.php/pm/article/view/15435 |
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