Comparison of antigen- and RT-PCR-based testing strategies for detection of SARS-CoV-2 in two high-exposure settings.
Surveillance testing for infectious disease is an important tool to combat disease transmission at the population level. During the SARS-CoV-2 pandemic, RT-PCR tests have been considered the gold standard due to their high sensitivity and specificity. However, RT-PCR tests for SARS-CoV-2 have been s...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2021-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0253407&type=printable |
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| _version_ | 1850127345487183872 |
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| author | Jay Love Megan T Wimmer Damon J A Toth Arthi Chandran Dilip Makhija Charles K Cooper Matthew H Samore Lindsay T Keegan |
| author_facet | Jay Love Megan T Wimmer Damon J A Toth Arthi Chandran Dilip Makhija Charles K Cooper Matthew H Samore Lindsay T Keegan |
| author_sort | Jay Love |
| collection | DOAJ |
| description | Surveillance testing for infectious disease is an important tool to combat disease transmission at the population level. During the SARS-CoV-2 pandemic, RT-PCR tests have been considered the gold standard due to their high sensitivity and specificity. However, RT-PCR tests for SARS-CoV-2 have been shown to return positive results when performed to individuals who are past the infectious stage of the disease. Meanwhile, antigen-based tests are often treated as a less accurate substitute for RT-PCR, however, new evidence suggests they may better reflect infectiousness. Consequently, the two test types may each be most optimally deployed in different settings. Here, we present an epidemiological model with surveillance testing and coordinated isolation in two congregate living settings (a nursing home and a university dormitory system) that considers test metrics with respect to viral culture, a proxy for infectiousness. Simulations show that antigen-based surveillance testing coupled with isolation greatly reduces disease burden and carries a lower economic cost than RT-PCR-based strategies. Antigen and RT-PCR tests perform different functions toward the goal of reducing infectious disease burden and should be used accordingly. |
| format | Article |
| id | doaj-art-8d256b2b04864cb1b173edf508854f41 |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-8d256b2b04864cb1b173edf508854f412025-08-20T02:33:43ZengPublic Library of Science (PLoS)PLoS ONE1932-62032021-01-01169e025340710.1371/journal.pone.0253407Comparison of antigen- and RT-PCR-based testing strategies for detection of SARS-CoV-2 in two high-exposure settings.Jay LoveMegan T WimmerDamon J A TothArthi ChandranDilip MakhijaCharles K CooperMatthew H SamoreLindsay T KeeganSurveillance testing for infectious disease is an important tool to combat disease transmission at the population level. During the SARS-CoV-2 pandemic, RT-PCR tests have been considered the gold standard due to their high sensitivity and specificity. However, RT-PCR tests for SARS-CoV-2 have been shown to return positive results when performed to individuals who are past the infectious stage of the disease. Meanwhile, antigen-based tests are often treated as a less accurate substitute for RT-PCR, however, new evidence suggests they may better reflect infectiousness. Consequently, the two test types may each be most optimally deployed in different settings. Here, we present an epidemiological model with surveillance testing and coordinated isolation in two congregate living settings (a nursing home and a university dormitory system) that considers test metrics with respect to viral culture, a proxy for infectiousness. Simulations show that antigen-based surveillance testing coupled with isolation greatly reduces disease burden and carries a lower economic cost than RT-PCR-based strategies. Antigen and RT-PCR tests perform different functions toward the goal of reducing infectious disease burden and should be used accordingly.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0253407&type=printable |
| spellingShingle | Jay Love Megan T Wimmer Damon J A Toth Arthi Chandran Dilip Makhija Charles K Cooper Matthew H Samore Lindsay T Keegan Comparison of antigen- and RT-PCR-based testing strategies for detection of SARS-CoV-2 in two high-exposure settings. PLoS ONE |
| title | Comparison of antigen- and RT-PCR-based testing strategies for detection of SARS-CoV-2 in two high-exposure settings. |
| title_full | Comparison of antigen- and RT-PCR-based testing strategies for detection of SARS-CoV-2 in two high-exposure settings. |
| title_fullStr | Comparison of antigen- and RT-PCR-based testing strategies for detection of SARS-CoV-2 in two high-exposure settings. |
| title_full_unstemmed | Comparison of antigen- and RT-PCR-based testing strategies for detection of SARS-CoV-2 in two high-exposure settings. |
| title_short | Comparison of antigen- and RT-PCR-based testing strategies for detection of SARS-CoV-2 in two high-exposure settings. |
| title_sort | comparison of antigen and rt pcr based testing strategies for detection of sars cov 2 in two high exposure settings |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0253407&type=printable |
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