Effect of Polypropylene Microplastic and Florfenicol Antibiotic on Some Hormonal and Haematological Biomarkers in Yellowfin Seabream (Acanthopagrus latus)

Microplastics (MPs) and antibiotics, such as florfenicol (FFC), are emerging pollutants affecting aquatic ecosystems. This research examined the separate and joint impacts of MPs and FFC on hematological and hormonal indicators in yellowfin seabream (Acanthopagrus latus). The fish were categorized i...

Full description

Saved in:
Bibliographic Details
Main Authors: Mehrnaz Shirmohammadi, Farahnaz Kianersi, Nima Shiry, Fatemeh Hekmatpour
Format: Article
Language:English
Published: Istanbul University Press 2025-04-01
Series:Aquatic Sciences and Engineering
Subjects:
Online Access:https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/726320695250486D8F625776B85D8637
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1849313065290432512
author Mehrnaz Shirmohammadi
Farahnaz Kianersi
Nima Shiry
Fatemeh Hekmatpour
author_facet Mehrnaz Shirmohammadi
Farahnaz Kianersi
Nima Shiry
Fatemeh Hekmatpour
author_sort Mehrnaz Shirmohammadi
collection DOAJ
description Microplastics (MPs) and antibiotics, such as florfenicol (FFC), are emerging pollutants affecting aquatic ecosystems. This research examined the separate and joint impacts of MPs and FFC on hematological and hormonal indicators in yellowfin seabream (Acanthopagrus latus). The fish were categorized into four groups according to their dietary treatments: a control group with a standard diet and three experimental groups receiving diets containing 15 mg/kg of FFC, 100 mg/kg of MPs, or both for 10-day. The ELISA method was used to measure hormones in plasma. Blood collection occurred on the first, fourth, seventh, and fourteenth days after feeding stopped to evaluate FFC concentration in the plasma and various hematological and hormonal parameters. Exposure to FFC and MPs, alone or together, considerably decreased red blood cell, hemoglobin levels, hematocrit values, mean corpuscular hemoglobin, mean corpuscular hemoglobin, white blood cell, lymphocyte, and erythropoietin concentrations. Neutrophil and cortisol levels increased. Mean corpuscular volume was elevated only in the group receiving both FFC and MPs. After a 14- day recovery period, all measured parameters returned to baseline levels in the FFC-only group. Co-exposed group showed the highest concentration of FFC in the plasma on the first day. The groups administered MPs, individually or collectively, exhibited a reduction in thyroid hormones. These findings indicate that both MPs and FFC induce anemia and stress in yellowfin seabream, with co-exposure exacerbating these effects. Although the toxic effects of FFC were temporary, the lasting presence of MPs indicates potential long-term risks.
format Article
id doaj-art-8d1e172690a0468abd07d5a355d0a37b
institution Kabale University
issn 2602-473X
language English
publishDate 2025-04-01
publisher Istanbul University Press
record_format Article
series Aquatic Sciences and Engineering
spelling doaj-art-8d1e172690a0468abd07d5a355d0a37b2025-08-20T03:52:52ZengIstanbul University PressAquatic Sciences and Engineering2602-473X2025-04-0140211612310.26650/ASE20251638853123456Effect of Polypropylene Microplastic and Florfenicol Antibiotic on Some Hormonal and Haematological Biomarkers in Yellowfin Seabream (Acanthopagrus latus)Mehrnaz Shirmohammadi0https://orcid.org/0009-0003-7140-0626Farahnaz Kianersi1https://orcid.org/0009-0001-3956-7990Nima Shiry2https://orcid.org/0000-0002-7838-269XFatemeh Hekmatpour3https://orcid.org/0000-0002-1184-5143South of Iran Aquaculture Research Institute, Ahvaz, IranSouth of Iran Aquaculture Research Institute, Ahvaz, IranPersian Gulf and Oman Sea Ecological Research Institute, Bandar-Abbas, IranSouth of Iran Aquaculture Research Institute, Ahvaz, IranMicroplastics (MPs) and antibiotics, such as florfenicol (FFC), are emerging pollutants affecting aquatic ecosystems. This research examined the separate and joint impacts of MPs and FFC on hematological and hormonal indicators in yellowfin seabream (Acanthopagrus latus). The fish were categorized into four groups according to their dietary treatments: a control group with a standard diet and three experimental groups receiving diets containing 15 mg/kg of FFC, 100 mg/kg of MPs, or both for 10-day. The ELISA method was used to measure hormones in plasma. Blood collection occurred on the first, fourth, seventh, and fourteenth days after feeding stopped to evaluate FFC concentration in the plasma and various hematological and hormonal parameters. Exposure to FFC and MPs, alone or together, considerably decreased red blood cell, hemoglobin levels, hematocrit values, mean corpuscular hemoglobin, mean corpuscular hemoglobin, white blood cell, lymphocyte, and erythropoietin concentrations. Neutrophil and cortisol levels increased. Mean corpuscular volume was elevated only in the group receiving both FFC and MPs. After a 14- day recovery period, all measured parameters returned to baseline levels in the FFC-only group. Co-exposed group showed the highest concentration of FFC in the plasma on the first day. The groups administered MPs, individually or collectively, exhibited a reduction in thyroid hormones. These findings indicate that both MPs and FFC induce anemia and stress in yellowfin seabream, with co-exposure exacerbating these effects. Although the toxic effects of FFC were temporary, the lasting presence of MPs indicates potential long-term risks.https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/726320695250486D8F625776B85D8637yellowfin seabreamacanthopagrus latusflorfenicolhaematological/ hormonal indicesmicroplastics
spellingShingle Mehrnaz Shirmohammadi
Farahnaz Kianersi
Nima Shiry
Fatemeh Hekmatpour
Effect of Polypropylene Microplastic and Florfenicol Antibiotic on Some Hormonal and Haematological Biomarkers in Yellowfin Seabream (Acanthopagrus latus)
Aquatic Sciences and Engineering
yellowfin seabream
acanthopagrus latus
florfenicol
haematological/ hormonal indices
microplastics
title Effect of Polypropylene Microplastic and Florfenicol Antibiotic on Some Hormonal and Haematological Biomarkers in Yellowfin Seabream (Acanthopagrus latus)
title_full Effect of Polypropylene Microplastic and Florfenicol Antibiotic on Some Hormonal and Haematological Biomarkers in Yellowfin Seabream (Acanthopagrus latus)
title_fullStr Effect of Polypropylene Microplastic and Florfenicol Antibiotic on Some Hormonal and Haematological Biomarkers in Yellowfin Seabream (Acanthopagrus latus)
title_full_unstemmed Effect of Polypropylene Microplastic and Florfenicol Antibiotic on Some Hormonal and Haematological Biomarkers in Yellowfin Seabream (Acanthopagrus latus)
title_short Effect of Polypropylene Microplastic and Florfenicol Antibiotic on Some Hormonal and Haematological Biomarkers in Yellowfin Seabream (Acanthopagrus latus)
title_sort effect of polypropylene microplastic and florfenicol antibiotic on some hormonal and haematological biomarkers in yellowfin seabream acanthopagrus latus
topic yellowfin seabream
acanthopagrus latus
florfenicol
haematological/ hormonal indices
microplastics
url https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/726320695250486D8F625776B85D8637
work_keys_str_mv AT mehrnazshirmohammadi effectofpolypropylenemicroplasticandflorfenicolantibioticonsomehormonalandhaematologicalbiomarkersinyellowfinseabreamacanthopagruslatus
AT farahnazkianersi effectofpolypropylenemicroplasticandflorfenicolantibioticonsomehormonalandhaematologicalbiomarkersinyellowfinseabreamacanthopagruslatus
AT nimashiry effectofpolypropylenemicroplasticandflorfenicolantibioticonsomehormonalandhaematologicalbiomarkersinyellowfinseabreamacanthopagruslatus
AT fatemehhekmatpour effectofpolypropylenemicroplasticandflorfenicolantibioticonsomehormonalandhaematologicalbiomarkersinyellowfinseabreamacanthopagruslatus