Strengthening the Effectiveness of the Candidate Influenza Vaccine by Combining Conserved Sequences of Hemagglutinin and M2 protein
The development of universal influenza vaccine - a vaccine directed to all subtypes of human influenza A viruses - is the really actual problem task. This paper presents the comparative characteristic of the specific activity of various recombinant proteins consisting of antigenic determinants of in...
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| Format: | Article |
| Language: | Russian |
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Numikom LLC
2017-06-01
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| Series: | Эпидемиология и вакцинопрофилактика |
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| Online Access: | https://www.epidemvac.ru/jour/article/view/278 |
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| author | L. M. Tsybalova L. A. Stepanova R. Yu. Kotlyarov E. A. Blokhina M. A. Shuklina E. S. Mardanova A. V. Korotkov M. V. Potapchuk N. V. Ravin |
| author_facet | L. M. Tsybalova L. A. Stepanova R. Yu. Kotlyarov E. A. Blokhina M. A. Shuklina E. S. Mardanova A. V. Korotkov M. V. Potapchuk N. V. Ravin |
| author_sort | L. M. Tsybalova |
| collection | DOAJ |
| description | The development of universal influenza vaccine - a vaccine directed to all subtypes of human influenza A viruses - is the really actual problem task. This paper presents the comparative characteristic of the specific activity of various recombinant proteins consisting of antigenic determinants of influenza A virus - the ectodomain of the M2 protein (M2e) and a fragment of the second subunit of the hemagglutinin (the amino acid sequence 76 - 130). Flagellin - Salmonella typhimurium protein was used as carrier protein and as adjuvant. We use two forms of flagellin: full size and with deleted hypervariable region. The proteins showed high immunogenicity, and the ability to prevent lethal infection of influenza virus in mice. Full-length flagellin with HA2 (76 - 130) and M2e on the C-terminus (protein Flg-HA2-4M2e) demonstrated the most protective properties. It provides 100% survival immunized mice that were challenge with a high dose of influenza A (H3N2) - 10 LD50. Proteins containing only full sized flagellin with M2e or flagellin truncated form with M2e at the C-terminus and HA2 within the hypervariable region, protected 75% of animals from lethal infection. Protein Flg-HA2-4M2e is promising for further study as a vaccine. |
| format | Article |
| id | doaj-art-8d1cf5003b634a79bd2df95b18cf0ba7 |
| institution | DOAJ |
| issn | 2073-3046 2619-0494 |
| language | Russian |
| publishDate | 2017-06-01 |
| publisher | Numikom LLC |
| record_format | Article |
| series | Эпидемиология и вакцинопрофилактика |
| spelling | doaj-art-8d1cf5003b634a79bd2df95b18cf0ba72025-08-20T03:21:19ZrusNumikom LLCЭпидемиология и вакцинопрофилактика2073-30462619-04942017-06-01163657010.31631/2073-3046-2017-16-3-65-70277Strengthening the Effectiveness of the Candidate Influenza Vaccine by Combining Conserved Sequences of Hemagglutinin and M2 proteinL. M. Tsybalova0L. A. Stepanova1R. Yu. Kotlyarov2E. A. Blokhina3M. A. Shuklina4E. S. Mardanova5A. V. Korotkov6M. V. Potapchuk7N. V. Ravin8Federal State Budget Institution Research «Institute of Influenza» of the Ministry of Healthcare of the Russian FederationFederal State Budget Institution Research «Institute of Influenza» of the Ministry of Healthcare of the Russian FederationFederal State Institution «The Federal Research Centre «Fundamentals of Biotechnology» of the Russian Academy of SciencesFederal State Institution «The Federal Research Centre «Fundamentals of Biotechnology» of the Russian Academy of SciencesFederal State Budget Institution Research «Institute of Influenza» of the Ministry of Healthcare of the Russian FederationFederal State Institution «The Federal Research Centre «Fundamentals of Biotechnology» of the Russian Academy of SciencesFederal State Budget Institution Research «Institute of Influenza» of the Ministry of Healthcare of the Russian FederationFederal State Budget Institution Research «Institute of Influenza» of the Ministry of Healthcare of the Russian FederationFederal State Institution «The Federal Research Centre «Fundamentals of Biotechnology» of the Russian Academy of SciencesThe development of universal influenza vaccine - a vaccine directed to all subtypes of human influenza A viruses - is the really actual problem task. This paper presents the comparative characteristic of the specific activity of various recombinant proteins consisting of antigenic determinants of influenza A virus - the ectodomain of the M2 protein (M2e) and a fragment of the second subunit of the hemagglutinin (the amino acid sequence 76 - 130). Flagellin - Salmonella typhimurium protein was used as carrier protein and as adjuvant. We use two forms of flagellin: full size and with deleted hypervariable region. The proteins showed high immunogenicity, and the ability to prevent lethal infection of influenza virus in mice. Full-length flagellin with HA2 (76 - 130) and M2e on the C-terminus (protein Flg-HA2-4M2e) demonstrated the most protective properties. It provides 100% survival immunized mice that were challenge with a high dose of influenza A (H3N2) - 10 LD50. Proteins containing only full sized flagellin with M2e or flagellin truncated form with M2e at the C-terminus and HA2 within the hypervariable region, protected 75% of animals from lethal infection. Protein Flg-HA2-4M2e is promising for further study as a vaccine.https://www.epidemvac.ru/jour/article/view/278гриппвакцинациярекомбинантный белокиммуногенностьпротективностьinfluenzavaccinationrecombinant proteinimmunogenicityprotection |
| spellingShingle | L. M. Tsybalova L. A. Stepanova R. Yu. Kotlyarov E. A. Blokhina M. A. Shuklina E. S. Mardanova A. V. Korotkov M. V. Potapchuk N. V. Ravin Strengthening the Effectiveness of the Candidate Influenza Vaccine by Combining Conserved Sequences of Hemagglutinin and M2 protein Эпидемиология и вакцинопрофилактика грипп вакцинация рекомбинантный белок иммуногенность протективность influenza vaccination recombinant protein immunogenicity protection |
| title | Strengthening the Effectiveness of the Candidate Influenza Vaccine by Combining Conserved Sequences of Hemagglutinin and M2 protein |
| title_full | Strengthening the Effectiveness of the Candidate Influenza Vaccine by Combining Conserved Sequences of Hemagglutinin and M2 protein |
| title_fullStr | Strengthening the Effectiveness of the Candidate Influenza Vaccine by Combining Conserved Sequences of Hemagglutinin and M2 protein |
| title_full_unstemmed | Strengthening the Effectiveness of the Candidate Influenza Vaccine by Combining Conserved Sequences of Hemagglutinin and M2 protein |
| title_short | Strengthening the Effectiveness of the Candidate Influenza Vaccine by Combining Conserved Sequences of Hemagglutinin and M2 protein |
| title_sort | strengthening the effectiveness of the candidate influenza vaccine by combining conserved sequences of hemagglutinin and m2 protein |
| topic | грипп вакцинация рекомбинантный белок иммуногенность протективность influenza vaccination recombinant protein immunogenicity protection |
| url | https://www.epidemvac.ru/jour/article/view/278 |
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