Induced Mutagenesis and Comparative Genomics of <i>Raoultella</i> sp. 64 for Enhanced Antimony Resistance and Biosorption
Antimony-resistant bacteria are potential natural resources for the bioremediation of mining soil pollution. A <i>Raoultella</i> sp. 64 strain was isolated from antimony-contaminated soil. To enhance its Sb resistance abilities, this strain was transported into space aboard the Shenzhou...
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2025-04-01
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| author | Tianhua Huang Shiran Cao Xiaohan Li Chuhan Wang Xiawei Peng |
| author_facet | Tianhua Huang Shiran Cao Xiaohan Li Chuhan Wang Xiawei Peng |
| author_sort | Tianhua Huang |
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| description | Antimony-resistant bacteria are potential natural resources for the bioremediation of mining soil pollution. A <i>Raoultella</i> sp. 64 strain was isolated from antimony-contaminated soil. To enhance its Sb resistance abilities, this strain was transported into space aboard the Shenzhou spacecraft for space breeding, resulting in a mutant strain, <i>Raoultella</i> sp. D9. The whole genomes of <i>Raoultella</i> sp. 64 and mutant strain <i>Raoultella</i> sp. D9 were sequenced, revealing the genomic information for the bacterium. Comparative genomic analysis was then carried out to identify differential functional genes. The adsorption conditions for Sb(III) were optimized and refined. Further, Fourier transform infrared spectroscopy (FTIR) was used to determine the adsorption of antimony. Results show that strain D9 exhibits a higher tolerance to Sb(III), and Sb resistance genes were identified in both <i>Raoultella</i> sp. 64 and D9. Analysis of the differential functional genes indicated that the increased copy number of <i>pls</i>X may lead to a higher lipid content in the cell membrane, thereby enhancing the cell’s resistance to heavy metals. Mutant strain D9 exhibited better biosorption capacity compared to strain 64. FTIR studies showed that key functional groups, including -OH, C-N, C-H, and C-O, are likely to have participated in Sb(III) biosorption. Further study of the differential functional genes could provide a basis for future research and the subsequent development of technologies for the remediation of Sb-contaminated sites. |
| format | Article |
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| spelling | doaj-art-8d17a3a6b7444411b336da0dd4c20ffa2025-08-20T02:18:04ZengMDPI AGMicroorganisms2076-26072025-04-0113488010.3390/microorganisms13040880Induced Mutagenesis and Comparative Genomics of <i>Raoultella</i> sp. 64 for Enhanced Antimony Resistance and BiosorptionTianhua Huang0Shiran Cao1Xiaohan Li2Chuhan Wang3Xiawei Peng4School of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, ChinaSchool of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, ChinaSchool of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, ChinaSchool of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, ChinaSchool of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, ChinaAntimony-resistant bacteria are potential natural resources for the bioremediation of mining soil pollution. A <i>Raoultella</i> sp. 64 strain was isolated from antimony-contaminated soil. To enhance its Sb resistance abilities, this strain was transported into space aboard the Shenzhou spacecraft for space breeding, resulting in a mutant strain, <i>Raoultella</i> sp. D9. The whole genomes of <i>Raoultella</i> sp. 64 and mutant strain <i>Raoultella</i> sp. D9 were sequenced, revealing the genomic information for the bacterium. Comparative genomic analysis was then carried out to identify differential functional genes. The adsorption conditions for Sb(III) were optimized and refined. Further, Fourier transform infrared spectroscopy (FTIR) was used to determine the adsorption of antimony. Results show that strain D9 exhibits a higher tolerance to Sb(III), and Sb resistance genes were identified in both <i>Raoultella</i> sp. 64 and D9. Analysis of the differential functional genes indicated that the increased copy number of <i>pls</i>X may lead to a higher lipid content in the cell membrane, thereby enhancing the cell’s resistance to heavy metals. Mutant strain D9 exhibited better biosorption capacity compared to strain 64. FTIR studies showed that key functional groups, including -OH, C-N, C-H, and C-O, are likely to have participated in Sb(III) biosorption. Further study of the differential functional genes could provide a basis for future research and the subsequent development of technologies for the remediation of Sb-contaminated sites.https://www.mdpi.com/2076-2607/13/4/880<i>Raoultella ornithinolytica</i>space breedingSb(III)-Oxidizing bacteriaantimony adsorptiondifferential functional genes |
| spellingShingle | Tianhua Huang Shiran Cao Xiaohan Li Chuhan Wang Xiawei Peng Induced Mutagenesis and Comparative Genomics of <i>Raoultella</i> sp. 64 for Enhanced Antimony Resistance and Biosorption Microorganisms <i>Raoultella ornithinolytica</i> space breeding Sb(III)-Oxidizing bacteria antimony adsorption differential functional genes |
| title | Induced Mutagenesis and Comparative Genomics of <i>Raoultella</i> sp. 64 for Enhanced Antimony Resistance and Biosorption |
| title_full | Induced Mutagenesis and Comparative Genomics of <i>Raoultella</i> sp. 64 for Enhanced Antimony Resistance and Biosorption |
| title_fullStr | Induced Mutagenesis and Comparative Genomics of <i>Raoultella</i> sp. 64 for Enhanced Antimony Resistance and Biosorption |
| title_full_unstemmed | Induced Mutagenesis and Comparative Genomics of <i>Raoultella</i> sp. 64 for Enhanced Antimony Resistance and Biosorption |
| title_short | Induced Mutagenesis and Comparative Genomics of <i>Raoultella</i> sp. 64 for Enhanced Antimony Resistance and Biosorption |
| title_sort | induced mutagenesis and comparative genomics of i raoultella i sp 64 for enhanced antimony resistance and biosorption |
| topic | <i>Raoultella ornithinolytica</i> space breeding Sb(III)-Oxidizing bacteria antimony adsorption differential functional genes |
| url | https://www.mdpi.com/2076-2607/13/4/880 |
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