Identification and analysis of functional regulatory elements and RNAi silencing genes in mandarin clementine (Citrus clementina)
This study aimed to identify the RNA interference-associated AGO, DCL, and RDR gene families in Citrus clementina using various bioinformatics approaches. To our knowledge, there has not yet been a genome-wide investigation into the AGO, DCL, and RDR gene families in Citrus clementina, even though t...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2025-12-01
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| Series: | Journal of Plant Interactions |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/17429145.2025.2503289 |
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| author | Maryam Noor Iqra Mehar Hadia Hussain Abdel-Rhman Z. Gaafar Mutwakel Dabiellil Tawaf Ali Shah |
| author_facet | Maryam Noor Iqra Mehar Hadia Hussain Abdel-Rhman Z. Gaafar Mutwakel Dabiellil Tawaf Ali Shah |
| author_sort | Maryam Noor |
| collection | DOAJ |
| description | This study aimed to identify the RNA interference-associated AGO, DCL, and RDR gene families in Citrus clementina using various bioinformatics approaches. To our knowledge, there has not yet been a genome-wide investigation into the AGO, DCL, and RDR gene families in Citrus clementina, even though these families have been recognized in other species. The investigation identified 12 CcAGOs, 5 CcDCLs, and 1 CcRDR gene within the Citrus clementina genome, corresponding to the RNAi genes found in the model organism Arabidopsis thaliana. The results showed significant homogeneity within the same gene family, with the majority of RNAi genes localized in the nucleus. Furthermore, the study revealed that 18 of the identified RNAi genes were significantly involved in RNA gene silencing processes. These findings provide valuable insights for the molecular exploration of RNAi genes, potentially aiding in the improvement of Citrus clementina crops in response to stress factors. |
| format | Article |
| id | doaj-art-8d1714f22f1a42849cd475afc95e9dfb |
| institution | OA Journals |
| issn | 1742-9145 1742-9153 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Plant Interactions |
| spelling | doaj-art-8d1714f22f1a42849cd475afc95e9dfb2025-08-20T01:51:01ZengTaylor & Francis GroupJournal of Plant Interactions1742-91451742-91532025-12-0120110.1080/17429145.2025.2503289Identification and analysis of functional regulatory elements and RNAi silencing genes in mandarin clementine (Citrus clementina)Maryam Noor0Iqra Mehar1Hadia Hussain2Abdel-Rhman Z. Gaafar3Mutwakel Dabiellil4Tawaf Ali Shah5Department of Biotechnology, University of Okara, Okara, PakistanDepartment of Botany, Government College University Faisalabad, Punjab, PakistanDepartment of Plant Molecular Biology and Genetic Engineering, School of LifeSciences, Nanjing Forestry University, Nanjing, People's Republic of ChinaDepartment of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi ArabiaDepartment of Biology, Upper Nile University, Malakal, South SudanCollege of Agriculture Engineering and Food Science, Shandong University of Technology, Zibo, People’s Republic of ChinaThis study aimed to identify the RNA interference-associated AGO, DCL, and RDR gene families in Citrus clementina using various bioinformatics approaches. To our knowledge, there has not yet been a genome-wide investigation into the AGO, DCL, and RDR gene families in Citrus clementina, even though these families have been recognized in other species. The investigation identified 12 CcAGOs, 5 CcDCLs, and 1 CcRDR gene within the Citrus clementina genome, corresponding to the RNAi genes found in the model organism Arabidopsis thaliana. The results showed significant homogeneity within the same gene family, with the majority of RNAi genes localized in the nucleus. Furthermore, the study revealed that 18 of the identified RNAi genes were significantly involved in RNA gene silencing processes. These findings provide valuable insights for the molecular exploration of RNAi genes, potentially aiding in the improvement of Citrus clementina crops in response to stress factors.https://www.tandfonline.com/doi/10.1080/17429145.2025.2503289ArgonauteCitrus clementinadicer-likegene silencinggenome-wide analysisRNA dependent RNA polymerase |
| spellingShingle | Maryam Noor Iqra Mehar Hadia Hussain Abdel-Rhman Z. Gaafar Mutwakel Dabiellil Tawaf Ali Shah Identification and analysis of functional regulatory elements and RNAi silencing genes in mandarin clementine (Citrus clementina) Journal of Plant Interactions Argonaute Citrus clementina dicer-like gene silencing genome-wide analysis RNA dependent RNA polymerase |
| title | Identification and analysis of functional regulatory elements and RNAi silencing genes in mandarin clementine (Citrus clementina) |
| title_full | Identification and analysis of functional regulatory elements and RNAi silencing genes in mandarin clementine (Citrus clementina) |
| title_fullStr | Identification and analysis of functional regulatory elements and RNAi silencing genes in mandarin clementine (Citrus clementina) |
| title_full_unstemmed | Identification and analysis of functional regulatory elements and RNAi silencing genes in mandarin clementine (Citrus clementina) |
| title_short | Identification and analysis of functional regulatory elements and RNAi silencing genes in mandarin clementine (Citrus clementina) |
| title_sort | identification and analysis of functional regulatory elements and rnai silencing genes in mandarin clementine citrus clementina |
| topic | Argonaute Citrus clementina dicer-like gene silencing genome-wide analysis RNA dependent RNA polymerase |
| url | https://www.tandfonline.com/doi/10.1080/17429145.2025.2503289 |
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