Characterization of Microbial Communities and Antimicrobial Resistance Genes in Urban Wastewater in Three Cities in Vietnam
The inappropriate usage of antibiotics is accelerating the prevalence of antimicrobial resistant bacteria (ARB) and antimicrobial resistance genes (ARGs) in Vietnam. Wastewater surveillance is a promising approach to monitor ARB and ARGs. In this study, ARB and ARGs were evaluated in domestic wastew...
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Japan Society on Water Environment
2024-01-01
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| Series: | Journal of Water and Environment Technology |
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https://www.jstage.jst.go.jp/article/jwet/22/6/22_24-075/_pdf
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| author | Thi My Hanh Vu Futoshi Kurisu Futoshi Hasebe Masato Suzuki Ikuro Kasuga |
| author_facet | Thi My Hanh Vu Futoshi Kurisu Futoshi Hasebe Masato Suzuki Ikuro Kasuga |
| author_sort | Thi My Hanh Vu |
| collection | DOAJ |
| description | The inappropriate usage of antibiotics is accelerating the prevalence of antimicrobial resistant bacteria (ARB) and antimicrobial resistance genes (ARGs) in Vietnam. Wastewater surveillance is a promising approach to monitor ARB and ARGs. In this study, ARB and ARGs were evaluated in domestic wastewater samples collected from three major cities (Hanoi, Hai Phong, and Bac Ninh) in northern Vietnam in November 2022. The ratios of cefotaxime-resistant Escherichia coli to total E. coli in domestic wastewater were relatively high (8.4% E5.7%). Quantitative PCR demonstrated that the relative abundance of the class 1 integron integrase gene to prokaryotic 16S rRNA genes ranged from 6.8 ÁE10∁E to 1.3 ÁE10∁E. In original wastewater samples, Arcobacter and Aeromonadaceae were dominant. Enterobacteriaceae, Enterococcaceae, and Acinetobacter became dominant after broth enrichment with meropenem, highlighting the prevalence of potential carbapenem-resistant bacteria in urban areas in Vietnam. PCR screening revealed that the enriched cultures had clinically important carbapenemase genes, such as blaNDM and blaKPC. A metagenomic analysis with hybrid capture enrichment revealed that the ARG compositions of influent samples in different cities were similar (i.e., similar ARGs were prevalent in urban areas). This study demonstrated the value of wastewater surveillance for evaluating characteristics of ARB and ARGs in urban areas in Vietnam. |
| format | Article |
| id | doaj-art-8d121d2ca81a4359b9d1d4a1afd0cd77 |
| institution | DOAJ |
| issn | 1348-2165 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Japan Society on Water Environment |
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| series | Journal of Water and Environment Technology |
| spelling | doaj-art-8d121d2ca81a4359b9d1d4a1afd0cd772025-08-20T03:11:45ZengJapan Society on Water EnvironmentJournal of Water and Environment Technology1348-21652024-01-0122630832010.2965/jwet.24-07510.2965/jwet.24-075Characterization of Microbial Communities and Antimicrobial Resistance Genes in Urban Wastewater in Three Cities in VietnamThi My Hanh Vu0Futoshi Kurisu1Futoshi Hasebe2Masato Suzuki3Ikuro Kasuga4 Graduate School of Engineering, The University of Tokyo, Tokyo, Japan Graduate School of Engineering, The University of Tokyo, Tokyo, Japan Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan Graduate School of Engineering, The University of Tokyo, Tokyo, Japan The inappropriate usage of antibiotics is accelerating the prevalence of antimicrobial resistant bacteria (ARB) and antimicrobial resistance genes (ARGs) in Vietnam. Wastewater surveillance is a promising approach to monitor ARB and ARGs. In this study, ARB and ARGs were evaluated in domestic wastewater samples collected from three major cities (Hanoi, Hai Phong, and Bac Ninh) in northern Vietnam in November 2022. The ratios of cefotaxime-resistant Escherichia coli to total E. coli in domestic wastewater were relatively high (8.4% E5.7%). Quantitative PCR demonstrated that the relative abundance of the class 1 integron integrase gene to prokaryotic 16S rRNA genes ranged from 6.8 ÁE10∁E to 1.3 ÁE10∁E. In original wastewater samples, Arcobacter and Aeromonadaceae were dominant. Enterobacteriaceae, Enterococcaceae, and Acinetobacter became dominant after broth enrichment with meropenem, highlighting the prevalence of potential carbapenem-resistant bacteria in urban areas in Vietnam. PCR screening revealed that the enriched cultures had clinically important carbapenemase genes, such as blaNDM and blaKPC. A metagenomic analysis with hybrid capture enrichment revealed that the ARG compositions of influent samples in different cities were similar (i.e., similar ARGs were prevalent in urban areas). This study demonstrated the value of wastewater surveillance for evaluating characteristics of ARB and ARGs in urban areas in Vietnam. https://www.jstage.jst.go.jp/article/jwet/22/6/22_24-075/_pdf antimicrobial resistance geneantimicrobial resistant bacteriacarbapenemasevietnamwastewater surveillance |
| spellingShingle | Thi My Hanh Vu Futoshi Kurisu Futoshi Hasebe Masato Suzuki Ikuro Kasuga Characterization of Microbial Communities and Antimicrobial Resistance Genes in Urban Wastewater in Three Cities in Vietnam Journal of Water and Environment Technology antimicrobial resistance gene antimicrobial resistant bacteria carbapenemase vietnam wastewater surveillance |
| title | Characterization of Microbial Communities and Antimicrobial Resistance Genes in Urban Wastewater in Three Cities in Vietnam |
| title_full | Characterization of Microbial Communities and Antimicrobial Resistance Genes in Urban Wastewater in Three Cities in Vietnam |
| title_fullStr | Characterization of Microbial Communities and Antimicrobial Resistance Genes in Urban Wastewater in Three Cities in Vietnam |
| title_full_unstemmed | Characterization of Microbial Communities and Antimicrobial Resistance Genes in Urban Wastewater in Three Cities in Vietnam |
| title_short | Characterization of Microbial Communities and Antimicrobial Resistance Genes in Urban Wastewater in Three Cities in Vietnam |
| title_sort | characterization of microbial communities and antimicrobial resistance genes in urban wastewater in three cities in vietnam |
| topic | antimicrobial resistance gene antimicrobial resistant bacteria carbapenemase vietnam wastewater surveillance |
| url |
https://www.jstage.jst.go.jp/article/jwet/22/6/22_24-075/_pdf
|
| work_keys_str_mv | AT thimyhanhvu characterizationofmicrobialcommunitiesandantimicrobialresistancegenesinurbanwastewaterinthreecitiesinvietnam AT futoshikurisu characterizationofmicrobialcommunitiesandantimicrobialresistancegenesinurbanwastewaterinthreecitiesinvietnam AT futoshihasebe characterizationofmicrobialcommunitiesandantimicrobialresistancegenesinurbanwastewaterinthreecitiesinvietnam AT masatosuzuki characterizationofmicrobialcommunitiesandantimicrobialresistancegenesinurbanwastewaterinthreecitiesinvietnam AT ikurokasuga characterizationofmicrobialcommunitiesandantimicrobialresistancegenesinurbanwastewaterinthreecitiesinvietnam |