Methanol Extract of Ficus trigonata Stem Bark Demonstrated Antiplasmodial Activity in Mice
Malaria, caused by Plasmodium parasites, remains a global health concern, with drug resistance complicating control efforts. Plant-derived compounds have emerged as potential antimalarial agents. This study explores the effectiveness of methanol extract of Ficus trigonata stem bark against Plasmodiu...
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College of Medicine, Al-Nahrain University
2024-03-01
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| Series: | Baghdad Journal of Biochemistry and Applied Biological Sciences |
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| Online Access: | https://www.bjbabs.org/index.php/bjbabs/article/view/269 |
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| author | Suleiman mahmoud Jada Yusuf Umar Abdullahi Usman Wurochekke |
| author_facet | Suleiman mahmoud Jada Yusuf Umar Abdullahi Usman Wurochekke |
| author_sort | Suleiman mahmoud Jada |
| collection | DOAJ |
| description | Malaria, caused by Plasmodium parasites, remains a global health concern, with drug resistance complicating control efforts. Plant-derived compounds have emerged as potential antimalarial agents. This study explores the effectiveness of methanol extract of Ficus trigonata stem bark against Plasmodium berghei in a murine model. Thirty (30) adult mice used in this study were divided into 5 groups of 6 mice per group. Group A was inoculated with P. berghei and not treated which served as negative control. Group B was inoculated and treated with 10mg/Kg body weight of chloroquine (standard control). Groups C, D and E were inoculated with Plasmodium berghei and treated with the extract in doses of 100, 200 and 300 mg/kg body weight respectively. Parameters such as Packed cell volume (PCV) was measured by hematocrit technique, Parasitemia levels were monitored by microscopy, and Chemo-suppression activity was calculated to assess antiplasmodial potential. The in vivo murine model that was used enables efficient preliminary screening with good predictive validity. Ficus trigonata stem bark extract showed dose-dependent antiplasmodial activity in Plasmodium berghei-infected mice. All doses (100-300mg/kg) significantly (p<0.05) increased the level of packed cell volume on days 1-3 post-treatment. Parasitemia levels reduced significantly (p<0.05), with 300 mg/kg approaching chloroquine efficacy. Impressive dose-dependent chemo-suppression reached 85.68% with the highest F. trigonata stem bark extract dose by day 4, nearing the 94.16% standard drug activity. These findings highlight the extract's potential as an antimalarial agent and contribute to the search for alternative treatments. |
| format | Article |
| id | doaj-art-8d0eabd67d4b430da93dc5c3c776fb9e |
| institution | OA Journals |
| issn | 2706-9915 |
| language | English |
| publishDate | 2024-03-01 |
| publisher | College of Medicine, Al-Nahrain University |
| record_format | Article |
| series | Baghdad Journal of Biochemistry and Applied Biological Sciences |
| spelling | doaj-art-8d0eabd67d4b430da93dc5c3c776fb9e2025-08-20T01:49:07ZengCollege of Medicine, Al-Nahrain UniversityBaghdad Journal of Biochemistry and Applied Biological Sciences2706-99152024-03-0151597110.47419/bjbabs.v5i01.269Methanol Extract of Ficus trigonata Stem Bark Demonstrated Antiplasmodial Activity in MiceSuleiman mahmoud Jada0https://orcid.org/0009-0006-5943-2328Yusuf Umar1https://orcid.org/0009-0004-7258-2940Abdullahi Usman Wurochekke2https://orcid.org/0009-0009-8801-3212Modibbo Adama University, Yola, Adamawa StateModibbo Adama University, Yola, Adamawa state, NigeriaModibbo Adama University, Yola, Adamawa state, NigeriaMalaria, caused by Plasmodium parasites, remains a global health concern, with drug resistance complicating control efforts. Plant-derived compounds have emerged as potential antimalarial agents. This study explores the effectiveness of methanol extract of Ficus trigonata stem bark against Plasmodium berghei in a murine model. Thirty (30) adult mice used in this study were divided into 5 groups of 6 mice per group. Group A was inoculated with P. berghei and not treated which served as negative control. Group B was inoculated and treated with 10mg/Kg body weight of chloroquine (standard control). Groups C, D and E were inoculated with Plasmodium berghei and treated with the extract in doses of 100, 200 and 300 mg/kg body weight respectively. Parameters such as Packed cell volume (PCV) was measured by hematocrit technique, Parasitemia levels were monitored by microscopy, and Chemo-suppression activity was calculated to assess antiplasmodial potential. The in vivo murine model that was used enables efficient preliminary screening with good predictive validity. Ficus trigonata stem bark extract showed dose-dependent antiplasmodial activity in Plasmodium berghei-infected mice. All doses (100-300mg/kg) significantly (p<0.05) increased the level of packed cell volume on days 1-3 post-treatment. Parasitemia levels reduced significantly (p<0.05), with 300 mg/kg approaching chloroquine efficacy. Impressive dose-dependent chemo-suppression reached 85.68% with the highest F. trigonata stem bark extract dose by day 4, nearing the 94.16% standard drug activity. These findings highlight the extract's potential as an antimalarial agent and contribute to the search for alternative treatments. https://www.bjbabs.org/index.php/bjbabs/article/view/269chemo-suppressionficus trigonatamalariaparasitemia and plasmodium bergheipacked cell volume |
| spellingShingle | Suleiman mahmoud Jada Yusuf Umar Abdullahi Usman Wurochekke Methanol Extract of Ficus trigonata Stem Bark Demonstrated Antiplasmodial Activity in Mice Baghdad Journal of Biochemistry and Applied Biological Sciences chemo-suppression ficus trigonata malaria parasitemia and plasmodium berghei packed cell volume |
| title | Methanol Extract of Ficus trigonata Stem Bark Demonstrated Antiplasmodial Activity in Mice |
| title_full | Methanol Extract of Ficus trigonata Stem Bark Demonstrated Antiplasmodial Activity in Mice |
| title_fullStr | Methanol Extract of Ficus trigonata Stem Bark Demonstrated Antiplasmodial Activity in Mice |
| title_full_unstemmed | Methanol Extract of Ficus trigonata Stem Bark Demonstrated Antiplasmodial Activity in Mice |
| title_short | Methanol Extract of Ficus trigonata Stem Bark Demonstrated Antiplasmodial Activity in Mice |
| title_sort | methanol extract of ficus trigonata stem bark demonstrated antiplasmodial activity in mice |
| topic | chemo-suppression ficus trigonata malaria parasitemia and plasmodium berghei packed cell volume |
| url | https://www.bjbabs.org/index.php/bjbabs/article/view/269 |
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