Ultrasensitive detection and quantification of bovine Deltapapillomavirus in the semen of healthy horses
Abstract BPV1, BPV2, BPV13, and BPV14 are all genotypes of bovine delta papillomaviruses (δPV), of which the first three cause infections in horses and are associated with equine sarcoids. However, BPV14 infection has never been reported in equine species. In this study, we examined 58 fresh and tha...
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Nature Portfolio
2025-01-01
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| author | Anna Cutarelli Francesca De Falco Francesco Serpe Simona Izzo Giovanna Fusco Cornel Catoi Sante Roperto |
| author_facet | Anna Cutarelli Francesca De Falco Francesco Serpe Simona Izzo Giovanna Fusco Cornel Catoi Sante Roperto |
| author_sort | Anna Cutarelli |
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| description | Abstract BPV1, BPV2, BPV13, and BPV14 are all genotypes of bovine delta papillomaviruses (δPV), of which the first three cause infections in horses and are associated with equine sarcoids. However, BPV14 infection has never been reported in equine species. In this study, we examined 58 fresh and thawed commercial semen samples from healthy stallions. In 34 (58.6%), bovine δPV DNA was detected and quantified using droplet digital polymerase chain reaction (ddPCR). Real time quantitative PCR (qPCR) was able to identify bovine δPV DNA in 5 samples (8.6%). Of the BPV-infected semen samples, 15 were positive for BPV2 (~ 44.1%) on ddPCR and 4 (~ 11.7%) on qPCR; 12 (~ 35.3%) for BPV14 on ddPCR and 1 (~ 3%) by qPCR; 4 (~ 11.7%) for BPV1 on ddPCR, whereas qPCR failed to reveal this infection; 3 (~ 8.8%) for BPV13 on ddPCR; and BPV13 infection was not detected by qPCR. Our study showed for the first time that BPV14 is an additional infectious agent potentially responsible for infection in horses, as its transcripts were detected and quantified in some semen samples. Large-scale BPV14 screening is necessary to provide substantial data on the molecular epidemiology for a better understanding of the geographical divergence of BPV14 prevalence in different areas and how widespread BPV14 is among equids. |
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| institution | DOAJ |
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| language | English |
| publishDate | 2025-01-01 |
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| spelling | doaj-art-8d04a1693ff5441cbaeabe801dab78ab2025-08-20T02:46:08ZengNature PortfolioScientific Reports2045-23222025-01-011511810.1038/s41598-024-81682-7Ultrasensitive detection and quantification of bovine Deltapapillomavirus in the semen of healthy horsesAnna Cutarelli0Francesca De Falco1Francesco Serpe2Simona Izzo3Giovanna Fusco4Cornel Catoi5Sante Roperto6Istituto Zooprofilattico Sperimentale del MezzogiornoDipartimento di Medicina Veterinaria e delle Produzioni Animali, Università degli Studi di Napoli Federico IIIstituto Zooprofilattico Sperimentale del MezzogiornoDipartimento di Medicina Veterinaria e delle Produzioni Animali, Università degli Studi di Napoli Federico IIIstituto Zooprofilattico Sperimentale del MezzogiornoDepartment of Pathology, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary MedicineDipartimento di Medicina Veterinaria e delle Produzioni Animali, Università degli Studi di Napoli Federico IIAbstract BPV1, BPV2, BPV13, and BPV14 are all genotypes of bovine delta papillomaviruses (δPV), of which the first three cause infections in horses and are associated with equine sarcoids. However, BPV14 infection has never been reported in equine species. In this study, we examined 58 fresh and thawed commercial semen samples from healthy stallions. In 34 (58.6%), bovine δPV DNA was detected and quantified using droplet digital polymerase chain reaction (ddPCR). Real time quantitative PCR (qPCR) was able to identify bovine δPV DNA in 5 samples (8.6%). Of the BPV-infected semen samples, 15 were positive for BPV2 (~ 44.1%) on ddPCR and 4 (~ 11.7%) on qPCR; 12 (~ 35.3%) for BPV14 on ddPCR and 1 (~ 3%) by qPCR; 4 (~ 11.7%) for BPV1 on ddPCR, whereas qPCR failed to reveal this infection; 3 (~ 8.8%) for BPV13 on ddPCR; and BPV13 infection was not detected by qPCR. Our study showed for the first time that BPV14 is an additional infectious agent potentially responsible for infection in horses, as its transcripts were detected and quantified in some semen samples. Large-scale BPV14 screening is necessary to provide substantial data on the molecular epidemiology for a better understanding of the geographical divergence of BPV14 prevalence in different areas and how widespread BPV14 is among equids.https://doi.org/10.1038/s41598-024-81682-7Bovine papillomavirusCommercial stallion semenDroplet digital PCR (ddPCR)Healthy stallionsReal time quantitative PCR (qPCR)Semen |
| spellingShingle | Anna Cutarelli Francesca De Falco Francesco Serpe Simona Izzo Giovanna Fusco Cornel Catoi Sante Roperto Ultrasensitive detection and quantification of bovine Deltapapillomavirus in the semen of healthy horses Scientific Reports Bovine papillomavirus Commercial stallion semen Droplet digital PCR (ddPCR) Healthy stallions Real time quantitative PCR (qPCR) Semen |
| title | Ultrasensitive detection and quantification of bovine Deltapapillomavirus in the semen of healthy horses |
| title_full | Ultrasensitive detection and quantification of bovine Deltapapillomavirus in the semen of healthy horses |
| title_fullStr | Ultrasensitive detection and quantification of bovine Deltapapillomavirus in the semen of healthy horses |
| title_full_unstemmed | Ultrasensitive detection and quantification of bovine Deltapapillomavirus in the semen of healthy horses |
| title_short | Ultrasensitive detection and quantification of bovine Deltapapillomavirus in the semen of healthy horses |
| title_sort | ultrasensitive detection and quantification of bovine deltapapillomavirus in the semen of healthy horses |
| topic | Bovine papillomavirus Commercial stallion semen Droplet digital PCR (ddPCR) Healthy stallions Real time quantitative PCR (qPCR) Semen |
| url | https://doi.org/10.1038/s41598-024-81682-7 |
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