Bioluminescent imaging of an oomycete pathogen empowers chemical selections and rational fungicide applications
Abstract Fungicides play an indispensable role in ensuring food security. However, rational chemical selection and fungicide precision application guidance remain constrained by the limitations in real-time monitoring of tracking pathogens within plant tissues. In the current study, we generated a g...
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| Format: | Article |
| Language: | English |
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BMC
2025-05-01
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| Series: | Plant Methods |
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| Online Access: | https://doi.org/10.1186/s13007-025-01374-9 |
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| author | Han Chen Jiana Mao Yujie Fang Waqas Raza Zhi Li Chongyuan Zhang Yingguang Zhu Yuanchao Wang Suomeng Dong |
| author_facet | Han Chen Jiana Mao Yujie Fang Waqas Raza Zhi Li Chongyuan Zhang Yingguang Zhu Yuanchao Wang Suomeng Dong |
| author_sort | Han Chen |
| collection | DOAJ |
| description | Abstract Fungicides play an indispensable role in ensuring food security. However, rational chemical selection and fungicide precision application guidance remain constrained by the limitations in real-time monitoring of tracking pathogens within plant tissues. In the current study, we generated a genetically stable Phytophthora infestans strain (PiLuc) expressing luciferase gene, which serves as a dual-mode quantification platform for both in vitro and in vivo throughput screening. Consequently, we designed a 96-well plate high-throughput screening system to assess compounds inhibitory efficacy using PiLuc. Crucially, bioluminescence imaging enabled visualization of PiLuc in potato leaves and tubers during early infection stage, which is invisible to the naked eye. Capitalizing on the semi non-destructive and visual advantages, we developed a system for fungicide bioavailability evaluation and dosage-response assessment in tuber tissues, integrating real-time dynamic monitoring of pathogen. The development of bioluminescent imaging of late blight pathogen establishes an enabling platform for high-throughput fungicide screening while improving the precision bioavailability assessments. |
| format | Article |
| id | doaj-art-8d03aedf286f4d2aaf4edad03a98c063 |
| institution | OA Journals |
| issn | 1746-4811 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Plant Methods |
| spelling | doaj-art-8d03aedf286f4d2aaf4edad03a98c0632025-08-20T02:15:16ZengBMCPlant Methods1746-48112025-05-0121111310.1186/s13007-025-01374-9Bioluminescent imaging of an oomycete pathogen empowers chemical selections and rational fungicide applicationsHan Chen0Jiana Mao1Yujie Fang2Waqas Raza3Zhi Li4Chongyuan Zhang5Yingguang Zhu6Yuanchao Wang7Suomeng Dong8State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural UniversityState Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural UniversityState Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural UniversityState Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural UniversityState Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural UniversityState Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural UniversityJiangsu Key Laboratory of Pesticide Science and Department of Chemistry, College of Sciences, Nanjing Agricultural UniversityState Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural UniversityState Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural UniversityAbstract Fungicides play an indispensable role in ensuring food security. However, rational chemical selection and fungicide precision application guidance remain constrained by the limitations in real-time monitoring of tracking pathogens within plant tissues. In the current study, we generated a genetically stable Phytophthora infestans strain (PiLuc) expressing luciferase gene, which serves as a dual-mode quantification platform for both in vitro and in vivo throughput screening. Consequently, we designed a 96-well plate high-throughput screening system to assess compounds inhibitory efficacy using PiLuc. Crucially, bioluminescence imaging enabled visualization of PiLuc in potato leaves and tubers during early infection stage, which is invisible to the naked eye. Capitalizing on the semi non-destructive and visual advantages, we developed a system for fungicide bioavailability evaluation and dosage-response assessment in tuber tissues, integrating real-time dynamic monitoring of pathogen. The development of bioluminescent imaging of late blight pathogen establishes an enabling platform for high-throughput fungicide screening while improving the precision bioavailability assessments.https://doi.org/10.1186/s13007-025-01374-9Bioluminescent imagingLuciferaseChemical selectionsQuantitative high-throughput screeningLate blight |
| spellingShingle | Han Chen Jiana Mao Yujie Fang Waqas Raza Zhi Li Chongyuan Zhang Yingguang Zhu Yuanchao Wang Suomeng Dong Bioluminescent imaging of an oomycete pathogen empowers chemical selections and rational fungicide applications Plant Methods Bioluminescent imaging Luciferase Chemical selections Quantitative high-throughput screening Late blight |
| title | Bioluminescent imaging of an oomycete pathogen empowers chemical selections and rational fungicide applications |
| title_full | Bioluminescent imaging of an oomycete pathogen empowers chemical selections and rational fungicide applications |
| title_fullStr | Bioluminescent imaging of an oomycete pathogen empowers chemical selections and rational fungicide applications |
| title_full_unstemmed | Bioluminescent imaging of an oomycete pathogen empowers chemical selections and rational fungicide applications |
| title_short | Bioluminescent imaging of an oomycete pathogen empowers chemical selections and rational fungicide applications |
| title_sort | bioluminescent imaging of an oomycete pathogen empowers chemical selections and rational fungicide applications |
| topic | Bioluminescent imaging Luciferase Chemical selections Quantitative high-throughput screening Late blight |
| url | https://doi.org/10.1186/s13007-025-01374-9 |
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