Estrogen-mediated modulation of sterile inflammatory markers and baroreflex sensitivity in ovariectomized female Wistar rats
ABSTRACT Objective: This study aims to explore the role of estrogen in providing cardioprotective benefits to premenopausal women, examining how hormonal differences between sexes influence the prevalence of cardiovascular diseases (CVDs) in women. Materials and methods: Eighteen female Wistar rat...
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Brazilian Society of Endocrinology and Metabolism
2025-01-01
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| Series: | Archives of Endocrinology and Metabolism |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2359-39972024001000301&lng=en&tlng=en |
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| author | Md. Iqbal Alam Naba Sami Aftab Alam Sheema Wazib Neha Dhyani Sher Afghan Mairaj Ahmed Ansari |
| author_facet | Md. Iqbal Alam Naba Sami Aftab Alam Sheema Wazib Neha Dhyani Sher Afghan Mairaj Ahmed Ansari |
| author_sort | Md. Iqbal Alam |
| collection | DOAJ |
| description | ABSTRACT Objective: This study aims to explore the role of estrogen in providing cardioprotective benefits to premenopausal women, examining how hormonal differences between sexes influence the prevalence of cardiovascular diseases (CVDs) in women. Materials and methods: Eighteen female Wistar rats were equally distributed into three treatment groups. Animals in Group I (sham-operated) and Group II (ovariectomized [OVX]) received oral saline solution at a dose of 2 mL/kg. Group III (OVX+E2) received oral E2 2 µg/mL/kg after ovariectomy. Hemodynamic parameters and baroreflex sensitivity were determined in all groups. Plasma levels of malondialdehyde (MDA), superoxide dismutase (SOD), and nitric oxide (NO) were measured, along with those of the inflammatory markers tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and high mobility group box-1 (HMGB-1). Results: The OVX group, compared with the sham-operated group, displayed significantly altered hemodynamic parameters and baroreflex sensitivity, along with elevated MDA levels and decreased SOD and NO levels. This group also had higher levels of inflammatory cytokines than the sham-operated group. In the absence of estrogen, these factors led to the advancement of cardiovascular abnormalities. In the OVX+E2 group, estrogen treatment considerably improved baroreflex sensitivity and hemodynamic profile while reducing the expression of inflammatory cytokines compared with the OVX group, demonstrating anti-inflammatory actions of estrogen. Conclusion: Estrogen mediates cardioprotection by improving baroreflex sensitivity in ovariectomized Wistar rats through modulation of the NO pathway. |
| format | Article |
| id | doaj-art-8cf491f25482417d8ea5883c5754822a |
| institution | Kabale University |
| issn | 2359-4292 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Brazilian Society of Endocrinology and Metabolism |
| record_format | Article |
| series | Archives of Endocrinology and Metabolism |
| spelling | doaj-art-8cf491f25482417d8ea5883c5754822a2025-01-21T07:45:39ZengBrazilian Society of Endocrinology and MetabolismArchives of Endocrinology and Metabolism2359-42922025-01-0168spe110.20945/2359-4292-2023-0521Estrogen-mediated modulation of sterile inflammatory markers and baroreflex sensitivity in ovariectomized female Wistar ratsMd. Iqbal Alamhttps://orcid.org/0000-0002-3285-7282Naba Samihttps://orcid.org/0000-0002-7479-2384Aftab Alamhttps://orcid.org/0000-0001-8089-6721Sheema Wazibhttps://orcid.org/0009-0006-9734-3238Neha Dhyanihttps://orcid.org/0000-0003-0853-5758Sher Afghanhttps://orcid.org/0009-0000-1598-4709Mairaj Ahmed Ansarihttps://orcid.org/0000-0003-0040-4147ABSTRACT Objective: This study aims to explore the role of estrogen in providing cardioprotective benefits to premenopausal women, examining how hormonal differences between sexes influence the prevalence of cardiovascular diseases (CVDs) in women. Materials and methods: Eighteen female Wistar rats were equally distributed into three treatment groups. Animals in Group I (sham-operated) and Group II (ovariectomized [OVX]) received oral saline solution at a dose of 2 mL/kg. Group III (OVX+E2) received oral E2 2 µg/mL/kg after ovariectomy. Hemodynamic parameters and baroreflex sensitivity were determined in all groups. Plasma levels of malondialdehyde (MDA), superoxide dismutase (SOD), and nitric oxide (NO) were measured, along with those of the inflammatory markers tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and high mobility group box-1 (HMGB-1). Results: The OVX group, compared with the sham-operated group, displayed significantly altered hemodynamic parameters and baroreflex sensitivity, along with elevated MDA levels and decreased SOD and NO levels. This group also had higher levels of inflammatory cytokines than the sham-operated group. In the absence of estrogen, these factors led to the advancement of cardiovascular abnormalities. In the OVX+E2 group, estrogen treatment considerably improved baroreflex sensitivity and hemodynamic profile while reducing the expression of inflammatory cytokines compared with the OVX group, demonstrating anti-inflammatory actions of estrogen. Conclusion: Estrogen mediates cardioprotection by improving baroreflex sensitivity in ovariectomized Wistar rats through modulation of the NO pathway.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2359-39972024001000301&lng=en&tlng=enCardiovascular diseasesbaroreflex sensitivityoxidative stressinflammatory cytokinesovariectomyestrogen |
| spellingShingle | Md. Iqbal Alam Naba Sami Aftab Alam Sheema Wazib Neha Dhyani Sher Afghan Mairaj Ahmed Ansari Estrogen-mediated modulation of sterile inflammatory markers and baroreflex sensitivity in ovariectomized female Wistar rats Archives of Endocrinology and Metabolism Cardiovascular diseases baroreflex sensitivity oxidative stress inflammatory cytokines ovariectomy estrogen |
| title | Estrogen-mediated modulation of sterile inflammatory markers and baroreflex sensitivity in ovariectomized female Wistar rats |
| title_full | Estrogen-mediated modulation of sterile inflammatory markers and baroreflex sensitivity in ovariectomized female Wistar rats |
| title_fullStr | Estrogen-mediated modulation of sterile inflammatory markers and baroreflex sensitivity in ovariectomized female Wistar rats |
| title_full_unstemmed | Estrogen-mediated modulation of sterile inflammatory markers and baroreflex sensitivity in ovariectomized female Wistar rats |
| title_short | Estrogen-mediated modulation of sterile inflammatory markers and baroreflex sensitivity in ovariectomized female Wistar rats |
| title_sort | estrogen mediated modulation of sterile inflammatory markers and baroreflex sensitivity in ovariectomized female wistar rats |
| topic | Cardiovascular diseases baroreflex sensitivity oxidative stress inflammatory cytokines ovariectomy estrogen |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2359-39972024001000301&lng=en&tlng=en |
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