Inhibition of NA+/H+ Exchanger 1 Attenuates Renal Dysfunction Induced by Advanced Glycation End Products in Rats

It has been recognized that sodium hydrogen exchanger 1 (NHE1) is involved in the development of diabetic nephropathy. The role of NHE1 in kidney dysfunction induced by advanced glycation end products (AGEs) remains unknown. Renal damage was induced by AGEs via tail vein injections in rats. Function...

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Main Authors: Peng Li, Geng-Rong Chen, Fu Wang, Ping Xu, Li-Ying Liu, Ya-Ling Yin, Shuang-Xi Wang
Format: Article
Language:English
Published: Wiley 2016-01-01
Series:Journal of Diabetes Research
Online Access:http://dx.doi.org/10.1155/2016/1802036
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author Peng Li
Geng-Rong Chen
Fu Wang
Ping Xu
Li-Ying Liu
Ya-Ling Yin
Shuang-Xi Wang
author_facet Peng Li
Geng-Rong Chen
Fu Wang
Ping Xu
Li-Ying Liu
Ya-Ling Yin
Shuang-Xi Wang
author_sort Peng Li
collection DOAJ
description It has been recognized that sodium hydrogen exchanger 1 (NHE1) is involved in the development of diabetic nephropathy. The role of NHE1 in kidney dysfunction induced by advanced glycation end products (AGEs) remains unknown. Renal damage was induced by AGEs via tail vein injections in rats. Function and morphology of kidney were determined. Compared to vehicle- or BSA-treated rats, AGEs caused abnormalities of kidney structures and functions in rats, accompanied with higher MDA level and lower GSH content. Gene expressions of NHE1 gene and TGF-β1 in the renal cortex and urine were also increased in AGEs-injected rats. Importantly, all these detrimental effects induced by AGEs were reversed by inhibition of NHE1 or suppression of oxidative stress. These pieces of data demonstrated that AGEs may activate NHE1 to induce renal damage, which is related to TGF-β1.
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id doaj-art-8ce6671500dc42fa8a03aa49425c53e6
institution Kabale University
issn 2314-6745
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language English
publishDate 2016-01-01
publisher Wiley
record_format Article
series Journal of Diabetes Research
spelling doaj-art-8ce6671500dc42fa8a03aa49425c53e62025-08-20T03:37:44ZengWileyJournal of Diabetes Research2314-67452314-67532016-01-01201610.1155/2016/18020361802036Inhibition of NA+/H+ Exchanger 1 Attenuates Renal Dysfunction Induced by Advanced Glycation End Products in RatsPeng Li0Geng-Rong Chen1Fu Wang2Ping Xu3Li-Ying Liu4Ya-Ling Yin5Shuang-Xi Wang6College of Pharmacy, Xinxiang Medical University, Xinxiang 453003, ChinaDepartment of Pharmacology, Pharmaceutical College, Central South University, Changsha 410078, ChinaThe Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Health, Qilu Hospital, School of Medicine, Shandong University, Jinan 250012, ChinaCollege of Pharmacy, Xinxiang Medical University, Xinxiang 453003, ChinaDepartment of Pharmacology, Pharmaceutical College, Central South University, Changsha 410078, ChinaCollege of Pharmacy, Xinxiang Medical University, Xinxiang 453003, ChinaCollege of Pharmacy, Xinxiang Medical University, Xinxiang 453003, ChinaIt has been recognized that sodium hydrogen exchanger 1 (NHE1) is involved in the development of diabetic nephropathy. The role of NHE1 in kidney dysfunction induced by advanced glycation end products (AGEs) remains unknown. Renal damage was induced by AGEs via tail vein injections in rats. Function and morphology of kidney were determined. Compared to vehicle- or BSA-treated rats, AGEs caused abnormalities of kidney structures and functions in rats, accompanied with higher MDA level and lower GSH content. Gene expressions of NHE1 gene and TGF-β1 in the renal cortex and urine were also increased in AGEs-injected rats. Importantly, all these detrimental effects induced by AGEs were reversed by inhibition of NHE1 or suppression of oxidative stress. These pieces of data demonstrated that AGEs may activate NHE1 to induce renal damage, which is related to TGF-β1.http://dx.doi.org/10.1155/2016/1802036
spellingShingle Peng Li
Geng-Rong Chen
Fu Wang
Ping Xu
Li-Ying Liu
Ya-Ling Yin
Shuang-Xi Wang
Inhibition of NA+/H+ Exchanger 1 Attenuates Renal Dysfunction Induced by Advanced Glycation End Products in Rats
Journal of Diabetes Research
title Inhibition of NA+/H+ Exchanger 1 Attenuates Renal Dysfunction Induced by Advanced Glycation End Products in Rats
title_full Inhibition of NA+/H+ Exchanger 1 Attenuates Renal Dysfunction Induced by Advanced Glycation End Products in Rats
title_fullStr Inhibition of NA+/H+ Exchanger 1 Attenuates Renal Dysfunction Induced by Advanced Glycation End Products in Rats
title_full_unstemmed Inhibition of NA+/H+ Exchanger 1 Attenuates Renal Dysfunction Induced by Advanced Glycation End Products in Rats
title_short Inhibition of NA+/H+ Exchanger 1 Attenuates Renal Dysfunction Induced by Advanced Glycation End Products in Rats
title_sort inhibition of na h exchanger 1 attenuates renal dysfunction induced by advanced glycation end products in rats
url http://dx.doi.org/10.1155/2016/1802036
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AT fuwang inhibitionofnahexchanger1attenuatesrenaldysfunctioninducedbyadvancedglycationendproductsinrats
AT pingxu inhibitionofnahexchanger1attenuatesrenaldysfunctioninducedbyadvancedglycationendproductsinrats
AT liyingliu inhibitionofnahexchanger1attenuatesrenaldysfunctioninducedbyadvancedglycationendproductsinrats
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