Dioscin‐loaded zein nanoparticles alleviate lipopolysaccharide‐induced acute kidney injury via the microRNA‐let 7i signalling pathways
Abstract The present study investigates the potential role of dioscin (DIO) in the lipopolysaccharide (LPS)‐induced kidney injury. For this purpose, DIO‐loaded zein nanoparticles (DIO‐ZNPs) were formulated and evaluated for physicochemical parameters. The DIO‐ZNPs exhibited a controlled release of d...
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Wiley
2021-07-01
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| Series: | IET Nanobiotechnology |
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| Online Access: | https://doi.org/10.1049/nbt2.12051 |
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| author | Yun Zhang Yuangen Li Changda Lin Jiequn Zhang Hanyuan Gao Jinhai Chen |
| author_facet | Yun Zhang Yuangen Li Changda Lin Jiequn Zhang Hanyuan Gao Jinhai Chen |
| author_sort | Yun Zhang |
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| description | Abstract The present study investigates the potential role of dioscin (DIO) in the lipopolysaccharide (LPS)‐induced kidney injury. For this purpose, DIO‐loaded zein nanoparticles (DIO‐ZNPs) were formulated and evaluated for physicochemical parameters. The DIO‐ZNPs exhibited a controlled release of drug compared with that of the free drug suspension. Results showed that the cell viability of NRK‐52E consistently decreased with the increase in LPS from 0.01 µg/ml to 2 µg/ml. When compared with LPS, DIO‐induced NPs showed 1.10‐, 1.32‐, 1.57‐ and 1.92‐fold increase in the cell viability for concentrations of 20 µg/ml, 50 µg/ml, 100 µg/ml and 200 µg/ml, respectively. DIO‐ZNPs exhibited the most remarkable recovery in the cell proliferation compared with free DIO as shown by the cellular morphology analysis. Furthermore, Annexin‐V staining analysis showed that the LPS‐treated cells possess the lowest green fluorescence indicating fewer viable cells, whereas DIO‐ZNPs exhibited the maximum green fluorescence comparable with that of the non‐treated cells indicating maximum cell viability. Furthermore, the results show that DIO‐ZNPs significantly increased the expression of miR‐let‐7i in the epithelial kidney cells, whereas the expression levels of TLR4 were significantly downregulated compared with that of the LPS‐treated cells. In conclusion, miR‐let‐7i could be an interesting therapeutic target and nanoparticle‐based DIO could be a potential candidate in the management of acute kidney injury |
| format | Article |
| id | doaj-art-8ce54ecf3a0448ff9ebf0e4c61e08046 |
| institution | OA Journals |
| issn | 1751-8741 1751-875X |
| language | English |
| publishDate | 2021-07-01 |
| publisher | Wiley |
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| spelling | doaj-art-8ce54ecf3a0448ff9ebf0e4c61e080462025-08-20T02:09:34ZengWileyIET Nanobiotechnology1751-87411751-875X2021-07-0115546547210.1049/nbt2.12051Dioscin‐loaded zein nanoparticles alleviate lipopolysaccharide‐induced acute kidney injury via the microRNA‐let 7i signalling pathwaysYun Zhang0Yuangen Li1Changda Lin2Jiequn Zhang3Hanyuan Gao4Jinhai Chen5Department of Renal Medicine Second Affiliated Hospital of Fujian Medical University Quanzhou Fujian ChinaDepartment of Renal Medicine Second Affiliated Hospital of Fujian Medical University Quanzhou Fujian ChinaDepartment of Renal Medicine Second Affiliated Hospital of Fujian Medical University Quanzhou Fujian ChinaDepartment of Renal Medicine Second Affiliated Hospital of Fujian Medical University Quanzhou Fujian ChinaDepartment of Renal Medicine Second Affiliated Hospital of Fujian Medical University Quanzhou Fujian ChinaDepartment of Renal Medicine Second Affiliated Hospital of Fujian Medical University Quanzhou Fujian ChinaAbstract The present study investigates the potential role of dioscin (DIO) in the lipopolysaccharide (LPS)‐induced kidney injury. For this purpose, DIO‐loaded zein nanoparticles (DIO‐ZNPs) were formulated and evaluated for physicochemical parameters. The DIO‐ZNPs exhibited a controlled release of drug compared with that of the free drug suspension. Results showed that the cell viability of NRK‐52E consistently decreased with the increase in LPS from 0.01 µg/ml to 2 µg/ml. When compared with LPS, DIO‐induced NPs showed 1.10‐, 1.32‐, 1.57‐ and 1.92‐fold increase in the cell viability for concentrations of 20 µg/ml, 50 µg/ml, 100 µg/ml and 200 µg/ml, respectively. DIO‐ZNPs exhibited the most remarkable recovery in the cell proliferation compared with free DIO as shown by the cellular morphology analysis. Furthermore, Annexin‐V staining analysis showed that the LPS‐treated cells possess the lowest green fluorescence indicating fewer viable cells, whereas DIO‐ZNPs exhibited the maximum green fluorescence comparable with that of the non‐treated cells indicating maximum cell viability. Furthermore, the results show that DIO‐ZNPs significantly increased the expression of miR‐let‐7i in the epithelial kidney cells, whereas the expression levels of TLR4 were significantly downregulated compared with that of the LPS‐treated cells. In conclusion, miR‐let‐7i could be an interesting therapeutic target and nanoparticle‐based DIO could be a potential candidate in the management of acute kidney injuryhttps://doi.org/10.1049/nbt2.12051biochemistrybiomedical materialscellular biophysicsdrug delivery systemsdrugsfluorescence |
| spellingShingle | Yun Zhang Yuangen Li Changda Lin Jiequn Zhang Hanyuan Gao Jinhai Chen Dioscin‐loaded zein nanoparticles alleviate lipopolysaccharide‐induced acute kidney injury via the microRNA‐let 7i signalling pathways IET Nanobiotechnology biochemistry biomedical materials cellular biophysics drug delivery systems drugs fluorescence |
| title | Dioscin‐loaded zein nanoparticles alleviate lipopolysaccharide‐induced acute kidney injury via the microRNA‐let 7i signalling pathways |
| title_full | Dioscin‐loaded zein nanoparticles alleviate lipopolysaccharide‐induced acute kidney injury via the microRNA‐let 7i signalling pathways |
| title_fullStr | Dioscin‐loaded zein nanoparticles alleviate lipopolysaccharide‐induced acute kidney injury via the microRNA‐let 7i signalling pathways |
| title_full_unstemmed | Dioscin‐loaded zein nanoparticles alleviate lipopolysaccharide‐induced acute kidney injury via the microRNA‐let 7i signalling pathways |
| title_short | Dioscin‐loaded zein nanoparticles alleviate lipopolysaccharide‐induced acute kidney injury via the microRNA‐let 7i signalling pathways |
| title_sort | dioscin loaded zein nanoparticles alleviate lipopolysaccharide induced acute kidney injury via the microrna let 7i signalling pathways |
| topic | biochemistry biomedical materials cellular biophysics drug delivery systems drugs fluorescence |
| url | https://doi.org/10.1049/nbt2.12051 |
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