Urinary GADD45G Protein Excretion Is Associated with IgA Nephropathy Progression
<b>Background</b>: Growth arrest and DNA damage 45G (GADD45G) is a family of proteins involved in DNA damage response and cell growth arrest. In this study, we show evidence that urinary GADD45G protein is associated with the progression of IgA nephropathy. <b>Methods</b>: Pa...
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2024-12-01
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| author | Min-Jeong Lee Hyunee Yim Ji Eun Park Inwhee Park Heungsoo Kim Gyu-Tae Shin |
| author_facet | Min-Jeong Lee Hyunee Yim Ji Eun Park Inwhee Park Heungsoo Kim Gyu-Tae Shin |
| author_sort | Min-Jeong Lee |
| collection | DOAJ |
| description | <b>Background</b>: Growth arrest and DNA damage 45G (GADD45G) is a family of proteins involved in DNA damage response and cell growth arrest. In this study, we show evidence that urinary GADD45G protein is associated with the progression of IgA nephropathy. <b>Methods</b>: Patients diagnosed with IgA nephropathy without reversible acute kidney injury at study initiation and with at least one subsequent serum creatinine (SCr) measurement were included. A 50% or greater increase in SCr level was used as an endpoint for the deterioration of renal function. Enzyme-linked immunosorbent assay (ELISA) was performed using a Human GADD45G ELISA kit. Renal biopsy tissues were stained with a monoclonal mouse anti-GADD45G antibody. <b>Results</b>: Forty-five patients whose renal biopsy revealed IgA nephropathy were enrolled. Urinary GADD45G and urinary protein concentrations were 1.26 [0.69–2.20] μg/g creatinine and 0.65 [0.24–1.60] g/g creatinine, respectively. Urinary GADD45G showed significant positive correlations with SCr-slopes and urinary protein. The SCr-slope of the highest tertile group of urinary GADD45G (above 1.95 μg/g creatinine) was significantly higher than that of the lowest tertile group (below 0.90 μg/g). Univariate Cox regression analysis showed that urinary GADD45G was significantly associated with deterioration of renal function. A Kaplan–Meier test showed a significant difference in event-free survival for deterioration of renal function between the highest urinary GADD45G tertile group and other tertile groups. The area under the receiver operating characteristics (ROC) curve indicated urinary GADD45G had a good performance in predicting renal outcome (cut-off point 1.67 μg/g, positive predictive value 36.8%, negative predictive value 100%). Immunohistochemistry showed that GADD45G was expressed across all pathologic grades of IgA nephropathy and mainly detected in the cytoplasm of renal tubules, whereas no staining was noted in normal tissues. <b>Conclusions</b>: Urinary GADD45G excretion was significantly associated with kidney disease progression in patients with IgA nephropathy. |
| format | Article |
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| institution | DOAJ |
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| language | English |
| publishDate | 2024-12-01 |
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| spelling | doaj-art-8cd2d0482b6d42aa8f160b2f963a86a62025-08-20T02:53:23ZengMDPI AGBiomedicines2227-90592024-12-011212284610.3390/biomedicines12122846Urinary GADD45G Protein Excretion Is Associated with IgA Nephropathy ProgressionMin-Jeong Lee0Hyunee Yim1Ji Eun Park2Inwhee Park3Heungsoo Kim4Gyu-Tae Shin5Department of Nephrology, Ajou University School of Medicine, Suwon 16499, Republic of KoreaDepartment of Pathology, Ajou University School of Medicine, Suwon 16499, Republic of KoreaDepartment of Nephrology, Ajou University School of Medicine, Suwon 16499, Republic of KoreaDepartment of Nephrology, Ajou University School of Medicine, Suwon 16499, Republic of KoreaDepartment of Nephrology, Ajou University School of Medicine, Suwon 16499, Republic of KoreaDepartment of Nephrology, Ajou University School of Medicine, Suwon 16499, Republic of Korea<b>Background</b>: Growth arrest and DNA damage 45G (GADD45G) is a family of proteins involved in DNA damage response and cell growth arrest. In this study, we show evidence that urinary GADD45G protein is associated with the progression of IgA nephropathy. <b>Methods</b>: Patients diagnosed with IgA nephropathy without reversible acute kidney injury at study initiation and with at least one subsequent serum creatinine (SCr) measurement were included. A 50% or greater increase in SCr level was used as an endpoint for the deterioration of renal function. Enzyme-linked immunosorbent assay (ELISA) was performed using a Human GADD45G ELISA kit. Renal biopsy tissues were stained with a monoclonal mouse anti-GADD45G antibody. <b>Results</b>: Forty-five patients whose renal biopsy revealed IgA nephropathy were enrolled. Urinary GADD45G and urinary protein concentrations were 1.26 [0.69–2.20] μg/g creatinine and 0.65 [0.24–1.60] g/g creatinine, respectively. Urinary GADD45G showed significant positive correlations with SCr-slopes and urinary protein. The SCr-slope of the highest tertile group of urinary GADD45G (above 1.95 μg/g creatinine) was significantly higher than that of the lowest tertile group (below 0.90 μg/g). Univariate Cox regression analysis showed that urinary GADD45G was significantly associated with deterioration of renal function. A Kaplan–Meier test showed a significant difference in event-free survival for deterioration of renal function between the highest urinary GADD45G tertile group and other tertile groups. The area under the receiver operating characteristics (ROC) curve indicated urinary GADD45G had a good performance in predicting renal outcome (cut-off point 1.67 μg/g, positive predictive value 36.8%, negative predictive value 100%). Immunohistochemistry showed that GADD45G was expressed across all pathologic grades of IgA nephropathy and mainly detected in the cytoplasm of renal tubules, whereas no staining was noted in normal tissues. <b>Conclusions</b>: Urinary GADD45G excretion was significantly associated with kidney disease progression in patients with IgA nephropathy.https://www.mdpi.com/2227-9059/12/12/2846growth arrest and DNA damage 45G (GADD45G)kidney biomarkerchronic kidney diseaseIgA nephropathy |
| spellingShingle | Min-Jeong Lee Hyunee Yim Ji Eun Park Inwhee Park Heungsoo Kim Gyu-Tae Shin Urinary GADD45G Protein Excretion Is Associated with IgA Nephropathy Progression Biomedicines growth arrest and DNA damage 45G (GADD45G) kidney biomarker chronic kidney disease IgA nephropathy |
| title | Urinary GADD45G Protein Excretion Is Associated with IgA Nephropathy Progression |
| title_full | Urinary GADD45G Protein Excretion Is Associated with IgA Nephropathy Progression |
| title_fullStr | Urinary GADD45G Protein Excretion Is Associated with IgA Nephropathy Progression |
| title_full_unstemmed | Urinary GADD45G Protein Excretion Is Associated with IgA Nephropathy Progression |
| title_short | Urinary GADD45G Protein Excretion Is Associated with IgA Nephropathy Progression |
| title_sort | urinary gadd45g protein excretion is associated with iga nephropathy progression |
| topic | growth arrest and DNA damage 45G (GADD45G) kidney biomarker chronic kidney disease IgA nephropathy |
| url | https://www.mdpi.com/2227-9059/12/12/2846 |
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