cDNA cloning and sequence analysis of coat protein genes of TMV and CMV isolated from tobacco in Yunnan province
The cDNA of coat protein gene and its 3′ non-coding region of tobacco mosaic virus and coat protein gene of cucumber mosaic virus isolated from the tobacco in Yunnan province was synthesized by RT-PCR. The amplified cDNA fragments were then cloned and sequenced. The fragment of TMV-YN comprises 684b...
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Zhejiang University Press
2000-05-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2000.03.0261 |
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| author | LI Fan ZHOU Xue-ping CHEN Hai-ru LI De-bao |
| author_facet | LI Fan ZHOU Xue-ping CHEN Hai-ru LI De-bao |
| author_sort | LI Fan |
| collection | DOAJ |
| description | The cDNA of coat protein gene and its 3′ non-coding region of tobacco mosaic virus and coat protein gene of cucumber mosaic virus isolated from the tobacco in Yunnan province was synthesized by RT-PCR. The amplified cDNA fragments were then cloned and sequenced. The fragment of TMV-YN comprises 684bp (EMBL accession number: AJ239099), including 480bp of CP gene encoding 158 amino acids and a 204bp of 3' non-coding region. Comparison either the nucleotide sequence of CP gene or that of 3' non-coding region demonstrated that the TMV-YN is identical to the TMV-U1 strain and TMV common strain in Korea, with 100% homology. The CP gene of CMV-YNa consists of 657nt encoding 218 amino acid (EMBL accession number: AJ239098). The nucleotide sequence of CMV-YNa has 96%, 93% and 92% nucleotide identities with CMV-RB, CMV-117F and CMV-Y, respectively. |
| format | Article |
| id | doaj-art-8cc72904cb734ecfaf3845ac96287a13 |
| institution | DOAJ |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2000-05-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-8cc72904cb734ecfaf3845ac96287a132025-08-20T03:15:38ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552000-05-012626126510.3785/1008-9209.2000.03.026110089209cDNA cloning and sequence analysis of coat protein genes of TMV and CMV isolated from tobacco in Yunnan provinceLI FanZHOU Xue-pingCHEN Hai-ruLI De-baoThe cDNA of coat protein gene and its 3′ non-coding region of tobacco mosaic virus and coat protein gene of cucumber mosaic virus isolated from the tobacco in Yunnan province was synthesized by RT-PCR. The amplified cDNA fragments were then cloned and sequenced. The fragment of TMV-YN comprises 684bp (EMBL accession number: AJ239099), including 480bp of CP gene encoding 158 amino acids and a 204bp of 3' non-coding region. Comparison either the nucleotide sequence of CP gene or that of 3' non-coding region demonstrated that the TMV-YN is identical to the TMV-U1 strain and TMV common strain in Korea, with 100% homology. The CP gene of CMV-YNa consists of 657nt encoding 218 amino acid (EMBL accession number: AJ239098). The nucleotide sequence of CMV-YNa has 96%, 93% and 92% nucleotide identities with CMV-RB, CMV-117F and CMV-Y, respectively.https://www.academax.com/doi/10.3785/1008-9209.2000.03.0261TMVCMVcoat protein gene 3′ non-coding regionsequence analysis |
| spellingShingle | LI Fan ZHOU Xue-ping CHEN Hai-ru LI De-bao cDNA cloning and sequence analysis of coat protein genes of TMV and CMV isolated from tobacco in Yunnan province 浙江大学学报. 农业与生命科学版 TMV CMV coat protein gene 3′ non-coding region sequence analysis |
| title | cDNA cloning and sequence analysis of coat protein genes of TMV and CMV isolated from tobacco in Yunnan province |
| title_full | cDNA cloning and sequence analysis of coat protein genes of TMV and CMV isolated from tobacco in Yunnan province |
| title_fullStr | cDNA cloning and sequence analysis of coat protein genes of TMV and CMV isolated from tobacco in Yunnan province |
| title_full_unstemmed | cDNA cloning and sequence analysis of coat protein genes of TMV and CMV isolated from tobacco in Yunnan province |
| title_short | cDNA cloning and sequence analysis of coat protein genes of TMV and CMV isolated from tobacco in Yunnan province |
| title_sort | cdna cloning and sequence analysis of coat protein genes of tmv and cmv isolated from tobacco in yunnan province |
| topic | TMV CMV coat protein gene 3′ non-coding region sequence analysis |
| url | https://www.academax.com/doi/10.3785/1008-9209.2000.03.0261 |
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