High-Risk Keratoplasty Using Conditioned Media of Mesenchymal Stem Cells in an Experiment
Purpose: to evaluate in experiment the dynamics and intensity of vascular sprouting in the donor cornea during keratoplasty against the background of pre-existing neoangiogenesis when using a conditioned medium of mesenchymal stem cells.Materials and methods. The object of the study was 15 rabbits (...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | Russian |
| Published: |
Ophthalmology Publishing Group
2024-10-01
|
| Series: | Oftalʹmologiâ |
| Subjects: | |
| Online Access: | https://www.ophthalmojournal.com/opht/article/view/2442 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849240966932725760 |
|---|---|
| author | A. V. Tereshchenko I. G. Trifanenkova A. A. Temnov Yu. D. Bulatova S. K. Demyanchenko А. N. Sklifas A. V. Shchatskikh D. A. Shataev R. B. Iolchiev |
| author_facet | A. V. Tereshchenko I. G. Trifanenkova A. A. Temnov Yu. D. Bulatova S. K. Demyanchenko А. N. Sklifas A. V. Shchatskikh D. A. Shataev R. B. Iolchiev |
| author_sort | A. V. Tereshchenko |
| collection | DOAJ |
| description | Purpose: to evaluate in experiment the dynamics and intensity of vascular sprouting in the donor cornea during keratoplasty against the background of pre-existing neoangiogenesis when using a conditioned medium of mesenchymal stem cells.Materials and methods. The object of the study was 15 rabbits (10 recipient rabbits, 5 donor rabbits) of the gray Chinchilla breed weighing 2.5–3.2 kg. The experiments were carried out in two stages. At the first stage, 10 animals were simulated with a unilateral thermal burn of the peripheral zone of the cornea with limbal involvement. Penetrating keratoplasty was performed using standard technology on the 15th day of the experiment. At the second stage, the recipient rabbits were divided into experimental and control groups. 5 rabbits, 5 eyes, in the control group and 5 rabbits, 5 eyes, in the main group. In the main group, instillations of paracrine factors of mesenchymal stem cells were used as postoperative treatment from the 1st to the 30th knocks four times. In the control group, treatment was carried out by instillation of antibiotics (Oftaquix) and keratoprotectors (Korneregel) three times for 30 days. Research methods included biomicroscopy, photographic recording of the anterior segment of the eye, examination using a Pentacam AXL device, and histological examination. The observation periods were 1st, 3rd, 7th, 14th, 30th day.Results. The results of postoperative treatment at the second stage of the experiment, as well as the results of histology, showed a pronounced antiangiogenic effect of the conditioned medium of mesenchymal stem cells. This was confirmed by the absence of pronounced vascularization of the donor cornea, the attenuation of the inflammatory reaction and the formation of a scar on the 30th day of drug instillation. In the control group, graft disease developed from the seventh day.Conclusion. The results obtained during the work seem promising. The use of the conditioned medium of mesenchymal stem cells demonstrated a sufficiently pronounced effectiveness compared with standard drug therapy in the postoperative period after high-risk end-to-end keratoplasty in experimental animals performed on a model of post-burn corneal neovascularization. |
| format | Article |
| id | doaj-art-8cc5315946f74ac9b1373962bdfc2409 |
| institution | Kabale University |
| issn | 1816-5095 2500-0845 |
| language | Russian |
| publishDate | 2024-10-01 |
| publisher | Ophthalmology Publishing Group |
| record_format | Article |
| series | Oftalʹmologiâ |
| spelling | doaj-art-8cc5315946f74ac9b1373962bdfc24092025-08-20T04:00:20ZrusOphthalmology Publishing GroupOftalʹmologiâ1816-50952500-08452024-10-0121360461110.18008/1816-5095-2024-3-604-6111184High-Risk Keratoplasty Using Conditioned Media of Mesenchymal Stem Cells in an ExperimentA. V. Tereshchenko0I. G. Trifanenkova1A. A. Temnov2Yu. D. Bulatova3S. K. Demyanchenko4А. N. Sklifas5A. V. Shchatskikh6D. A. Shataev7R. B. Iolchiev8Kaluga branch of the S. Fyodorov Eye Microsurgery Federal State Institution; Kaluga State University named after K.E. TsiolkovskiKaluga branch of the S. Fyodorov Eye Microsurgery Federal State Institution; Kaluga State University named after K.E. TsiolkovskiInstitute of Cell Biophysics of the Russian Academy of Sciences is a separate division of the “Federal Research Center “Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences”Kaluga branch of the S. Fyodorov Eye Microsurgery Federal State InstitutionKaluga branch of the S. Fyodorov Eye Microsurgery Federal State InstitutionInstitute of Cell Biophysics of the Russian Academy of Sciences is a separate division of the “Federal Research Center “Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences”The S. Fyodorov Eye Microsurgery Federal State InstitutionKaluga branch of the S. Fyodorov Eye Microsurgery Federal State InstitutionKaluga branch of the S. Fyodorov Eye Microsurgery Federal State InstitutionPurpose: to evaluate in experiment the dynamics and intensity of vascular sprouting in the donor cornea during keratoplasty against the background of pre-existing neoangiogenesis when using a conditioned medium of mesenchymal stem cells.Materials and methods. The object of the study was 15 rabbits (10 recipient rabbits, 5 donor rabbits) of the gray Chinchilla breed weighing 2.5–3.2 kg. The experiments were carried out in two stages. At the first stage, 10 animals were simulated with a unilateral thermal burn of the peripheral zone of the cornea with limbal involvement. Penetrating keratoplasty was performed using standard technology on the 15th day of the experiment. At the second stage, the recipient rabbits were divided into experimental and control groups. 5 rabbits, 5 eyes, in the control group and 5 rabbits, 5 eyes, in the main group. In the main group, instillations of paracrine factors of mesenchymal stem cells were used as postoperative treatment from the 1st to the 30th knocks four times. In the control group, treatment was carried out by instillation of antibiotics (Oftaquix) and keratoprotectors (Korneregel) three times for 30 days. Research methods included biomicroscopy, photographic recording of the anterior segment of the eye, examination using a Pentacam AXL device, and histological examination. The observation periods were 1st, 3rd, 7th, 14th, 30th day.Results. The results of postoperative treatment at the second stage of the experiment, as well as the results of histology, showed a pronounced antiangiogenic effect of the conditioned medium of mesenchymal stem cells. This was confirmed by the absence of pronounced vascularization of the donor cornea, the attenuation of the inflammatory reaction and the formation of a scar on the 30th day of drug instillation. In the control group, graft disease developed from the seventh day.Conclusion. The results obtained during the work seem promising. The use of the conditioned medium of mesenchymal stem cells demonstrated a sufficiently pronounced effectiveness compared with standard drug therapy in the postoperative period after high-risk end-to-end keratoplasty in experimental animals performed on a model of post-burn corneal neovascularization.https://www.ophthalmojournal.com/opht/article/view/2442mesenchymal stem cellsconditioned mediumcorneal neovascularizationhigh-risk keratoplastyexperiment |
| spellingShingle | A. V. Tereshchenko I. G. Trifanenkova A. A. Temnov Yu. D. Bulatova S. K. Demyanchenko А. N. Sklifas A. V. Shchatskikh D. A. Shataev R. B. Iolchiev High-Risk Keratoplasty Using Conditioned Media of Mesenchymal Stem Cells in an Experiment Oftalʹmologiâ mesenchymal stem cells conditioned medium corneal neovascularization high-risk keratoplasty experiment |
| title | High-Risk Keratoplasty Using Conditioned Media of Mesenchymal Stem Cells in an Experiment |
| title_full | High-Risk Keratoplasty Using Conditioned Media of Mesenchymal Stem Cells in an Experiment |
| title_fullStr | High-Risk Keratoplasty Using Conditioned Media of Mesenchymal Stem Cells in an Experiment |
| title_full_unstemmed | High-Risk Keratoplasty Using Conditioned Media of Mesenchymal Stem Cells in an Experiment |
| title_short | High-Risk Keratoplasty Using Conditioned Media of Mesenchymal Stem Cells in an Experiment |
| title_sort | high risk keratoplasty using conditioned media of mesenchymal stem cells in an experiment |
| topic | mesenchymal stem cells conditioned medium corneal neovascularization high-risk keratoplasty experiment |
| url | https://www.ophthalmojournal.com/opht/article/view/2442 |
| work_keys_str_mv | AT avtereshchenko highriskkeratoplastyusingconditionedmediaofmesenchymalstemcellsinanexperiment AT igtrifanenkova highriskkeratoplastyusingconditionedmediaofmesenchymalstemcellsinanexperiment AT aatemnov highriskkeratoplastyusingconditionedmediaofmesenchymalstemcellsinanexperiment AT yudbulatova highriskkeratoplastyusingconditionedmediaofmesenchymalstemcellsinanexperiment AT skdemyanchenko highriskkeratoplastyusingconditionedmediaofmesenchymalstemcellsinanexperiment AT ansklifas highriskkeratoplastyusingconditionedmediaofmesenchymalstemcellsinanexperiment AT avshchatskikh highriskkeratoplastyusingconditionedmediaofmesenchymalstemcellsinanexperiment AT dashataev highriskkeratoplastyusingconditionedmediaofmesenchymalstemcellsinanexperiment AT rbiolchiev highriskkeratoplastyusingconditionedmediaofmesenchymalstemcellsinanexperiment |