Comparative gene expression pattern of immune-related genes using dual-color RT-MLPA in the lesions of cutaneous leishmaniasis caused by L. major and L. tropica.
Cutaneous leishmaniasis (CL) is the most prevalent type of leishmaniasis disease and causes skin lesions, mainly ulcers, on exposed parts of the body. The Americas, Mediterranean basin, Middle East, and Central Asia account for approximately 95% of all CL cases. Leishmania (L.) major and L. tropica...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2025-03-01
|
| Series: | PLoS Neglected Tropical Diseases |
| Online Access: | https://doi.org/10.1371/journal.pntd.0012812 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849327832111513600 |
|---|---|
| author | Nasrin Masoudzadeh Mouad Ait Kbaich Suzanne van Veen Björn Andersson Marielle C Haks Josefine Persson Vahid Mashayekhi Goyonlo Shima Hadifar Reza Erfanian Salim Idris Mhaidi Myriam Riyad Khadija Akarid Ali M Harandi Tom Hm Ottenhoff Meryem Lemrani Sima Rafati |
| author_facet | Nasrin Masoudzadeh Mouad Ait Kbaich Suzanne van Veen Björn Andersson Marielle C Haks Josefine Persson Vahid Mashayekhi Goyonlo Shima Hadifar Reza Erfanian Salim Idris Mhaidi Myriam Riyad Khadija Akarid Ali M Harandi Tom Hm Ottenhoff Meryem Lemrani Sima Rafati |
| author_sort | Nasrin Masoudzadeh |
| collection | DOAJ |
| description | Cutaneous leishmaniasis (CL) is the most prevalent type of leishmaniasis disease and causes skin lesions, mainly ulcers, on exposed parts of the body. The Americas, Mediterranean basin, Middle East, and Central Asia account for approximately 95% of all CL cases. Leishmania (L.) major and L. tropica are the most significant species causing CL. A better understanding of the molecular mechanisms of CL caused by Leishmania parasite species in patients' skin lesions may help inform intervention approaches. Using dual-color reverse transcriptase multiplex ligation-dependent probe amplification (dcRT-MLPA), we evaluated the expression of 144 host immune-related genes in lesions from CL patients infected with two Leishmania species, L. major and L. tropica, in Morocco and Iran, respectively. Distinct gene expression patterns were identified in the lesions of patients infected with L. major and L. tropica. The results revealed that L. tropica-infected patients had rather more significant gene expression than L. major-infected patients relative to healthy volunteers. However, CD14 and IFI6 (interferon alpha inducible protein 6), were two common genes expressed in the lesions of patients infected with L. major and L. tropica. Our analysis revealed that gene expression changes related to the IFN signaling pathway were significant in both lesion groups. This research advances our understanding of the host immune response to zoonotic and anthroponotic leishmaniasis and shows immune transcript signatures in the skin lesions of CL patients infected with L. major and L. tropica. These findings can inform further investigation into the processes underpinning immunity and immunopathology of CL caused by L. major and L. tropica. |
| format | Article |
| id | doaj-art-8cbdf031e013415b962c99c0efaba22d |
| institution | Kabale University |
| issn | 1935-2727 1935-2735 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Neglected Tropical Diseases |
| spelling | doaj-art-8cbdf031e013415b962c99c0efaba22d2025-08-20T03:47:45ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352025-03-01193e001281210.1371/journal.pntd.0012812Comparative gene expression pattern of immune-related genes using dual-color RT-MLPA in the lesions of cutaneous leishmaniasis caused by L. major and L. tropica.Nasrin MasoudzadehMouad Ait KbaichSuzanne van VeenBjörn AnderssonMarielle C HaksJosefine PerssonVahid Mashayekhi GoyonloShima HadifarReza Erfanian SalimIdris MhaidiMyriam RiyadKhadija AkaridAli M HarandiTom Hm OttenhoffMeryem LemraniSima RafatiCutaneous leishmaniasis (CL) is the most prevalent type of leishmaniasis disease and causes skin lesions, mainly ulcers, on exposed parts of the body. The Americas, Mediterranean basin, Middle East, and Central Asia account for approximately 95% of all CL cases. Leishmania (L.) major and L. tropica are the most significant species causing CL. A better understanding of the molecular mechanisms of CL caused by Leishmania parasite species in patients' skin lesions may help inform intervention approaches. Using dual-color reverse transcriptase multiplex ligation-dependent probe amplification (dcRT-MLPA), we evaluated the expression of 144 host immune-related genes in lesions from CL patients infected with two Leishmania species, L. major and L. tropica, in Morocco and Iran, respectively. Distinct gene expression patterns were identified in the lesions of patients infected with L. major and L. tropica. The results revealed that L. tropica-infected patients had rather more significant gene expression than L. major-infected patients relative to healthy volunteers. However, CD14 and IFI6 (interferon alpha inducible protein 6), were two common genes expressed in the lesions of patients infected with L. major and L. tropica. Our analysis revealed that gene expression changes related to the IFN signaling pathway were significant in both lesion groups. This research advances our understanding of the host immune response to zoonotic and anthroponotic leishmaniasis and shows immune transcript signatures in the skin lesions of CL patients infected with L. major and L. tropica. These findings can inform further investigation into the processes underpinning immunity and immunopathology of CL caused by L. major and L. tropica.https://doi.org/10.1371/journal.pntd.0012812 |
| spellingShingle | Nasrin Masoudzadeh Mouad Ait Kbaich Suzanne van Veen Björn Andersson Marielle C Haks Josefine Persson Vahid Mashayekhi Goyonlo Shima Hadifar Reza Erfanian Salim Idris Mhaidi Myriam Riyad Khadija Akarid Ali M Harandi Tom Hm Ottenhoff Meryem Lemrani Sima Rafati Comparative gene expression pattern of immune-related genes using dual-color RT-MLPA in the lesions of cutaneous leishmaniasis caused by L. major and L. tropica. PLoS Neglected Tropical Diseases |
| title | Comparative gene expression pattern of immune-related genes using dual-color RT-MLPA in the lesions of cutaneous leishmaniasis caused by L. major and L. tropica. |
| title_full | Comparative gene expression pattern of immune-related genes using dual-color RT-MLPA in the lesions of cutaneous leishmaniasis caused by L. major and L. tropica. |
| title_fullStr | Comparative gene expression pattern of immune-related genes using dual-color RT-MLPA in the lesions of cutaneous leishmaniasis caused by L. major and L. tropica. |
| title_full_unstemmed | Comparative gene expression pattern of immune-related genes using dual-color RT-MLPA in the lesions of cutaneous leishmaniasis caused by L. major and L. tropica. |
| title_short | Comparative gene expression pattern of immune-related genes using dual-color RT-MLPA in the lesions of cutaneous leishmaniasis caused by L. major and L. tropica. |
| title_sort | comparative gene expression pattern of immune related genes using dual color rt mlpa in the lesions of cutaneous leishmaniasis caused by l major and l tropica |
| url | https://doi.org/10.1371/journal.pntd.0012812 |
| work_keys_str_mv | AT nasrinmasoudzadeh comparativegeneexpressionpatternofimmunerelatedgenesusingdualcolorrtmlpainthelesionsofcutaneousleishmaniasiscausedbylmajorandltropica AT mouadaitkbaich comparativegeneexpressionpatternofimmunerelatedgenesusingdualcolorrtmlpainthelesionsofcutaneousleishmaniasiscausedbylmajorandltropica AT suzannevanveen comparativegeneexpressionpatternofimmunerelatedgenesusingdualcolorrtmlpainthelesionsofcutaneousleishmaniasiscausedbylmajorandltropica AT bjornandersson comparativegeneexpressionpatternofimmunerelatedgenesusingdualcolorrtmlpainthelesionsofcutaneousleishmaniasiscausedbylmajorandltropica AT mariellechaks comparativegeneexpressionpatternofimmunerelatedgenesusingdualcolorrtmlpainthelesionsofcutaneousleishmaniasiscausedbylmajorandltropica AT josefinepersson comparativegeneexpressionpatternofimmunerelatedgenesusingdualcolorrtmlpainthelesionsofcutaneousleishmaniasiscausedbylmajorandltropica AT vahidmashayekhigoyonlo comparativegeneexpressionpatternofimmunerelatedgenesusingdualcolorrtmlpainthelesionsofcutaneousleishmaniasiscausedbylmajorandltropica AT shimahadifar comparativegeneexpressionpatternofimmunerelatedgenesusingdualcolorrtmlpainthelesionsofcutaneousleishmaniasiscausedbylmajorandltropica AT rezaerfaniansalim comparativegeneexpressionpatternofimmunerelatedgenesusingdualcolorrtmlpainthelesionsofcutaneousleishmaniasiscausedbylmajorandltropica AT idrismhaidi comparativegeneexpressionpatternofimmunerelatedgenesusingdualcolorrtmlpainthelesionsofcutaneousleishmaniasiscausedbylmajorandltropica AT myriamriyad comparativegeneexpressionpatternofimmunerelatedgenesusingdualcolorrtmlpainthelesionsofcutaneousleishmaniasiscausedbylmajorandltropica AT khadijaakarid comparativegeneexpressionpatternofimmunerelatedgenesusingdualcolorrtmlpainthelesionsofcutaneousleishmaniasiscausedbylmajorandltropica AT alimharandi comparativegeneexpressionpatternofimmunerelatedgenesusingdualcolorrtmlpainthelesionsofcutaneousleishmaniasiscausedbylmajorandltropica AT tomhmottenhoff comparativegeneexpressionpatternofimmunerelatedgenesusingdualcolorrtmlpainthelesionsofcutaneousleishmaniasiscausedbylmajorandltropica AT meryemlemrani comparativegeneexpressionpatternofimmunerelatedgenesusingdualcolorrtmlpainthelesionsofcutaneousleishmaniasiscausedbylmajorandltropica AT simarafati comparativegeneexpressionpatternofimmunerelatedgenesusingdualcolorrtmlpainthelesionsofcutaneousleishmaniasiscausedbylmajorandltropica |