Right ventricular volume overload reboots cardiomyocyte proliferation via immune responses
Abstract Background Right ventricular volume overload (RVVO) is one of the most important hemodynamic characteristics in children with congenital heart disease (CHD) and heart failure, and cardiomyocyte (CM) proliferation is one of the most vital factors for improving cardiac performance. However, w...
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| Format: | Article |
| Language: | English |
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BMC
2024-11-01
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| Series: | Journal of Translational Medicine |
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| Online Access: | https://doi.org/10.1186/s12967-024-05839-8 |
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| author | Chunxia Zhou Yuqing Hu Zhuoya Dong Zheng Wang Sixie Zheng Debao Li Yingying Xiao Dian Chen Hao Chen Sijuan Sun Lincai Ye Haibo Zhang |
| author_facet | Chunxia Zhou Yuqing Hu Zhuoya Dong Zheng Wang Sixie Zheng Debao Li Yingying Xiao Dian Chen Hao Chen Sijuan Sun Lincai Ye Haibo Zhang |
| author_sort | Chunxia Zhou |
| collection | DOAJ |
| description | Abstract Background Right ventricular volume overload (RVVO) is one of the most important hemodynamic characteristics in children with congenital heart disease (CHD) and heart failure, and cardiomyocyte (CM) proliferation is one of the most vital factors for improving cardiac performance. However, whether and how RVVO reboots CM proliferation remains elusive. Methods and results We first created a neonatal RVVO mouse model via abdominal aorta and inferior vena cava-fistula microsurgery at postnatal day 7 (P7), the edge of CM proliferation window. We subsequently performed bulk RNA-seq, single cell RNA-seq/flow cytometry, and immunofluorescence staining on the right ventricles (RV) of RVVO mice at P14/P21, defined as prepubertal stage, revealing that RVVO temporarily reboots prepubertal CM proliferation via immune responses. Conclusions In considering the importance of RVVO and CM proliferation, this study may bring an opportunity to create a novel paradigm to treat pediatric CHDs or heart failure. Graphical Abstract |
| format | Article |
| id | doaj-art-8cb7212a1be34dd5a2bf7187fec804a0 |
| institution | DOAJ |
| issn | 1479-5876 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Translational Medicine |
| spelling | doaj-art-8cb7212a1be34dd5a2bf7187fec804a02025-08-20T02:49:09ZengBMCJournal of Translational Medicine1479-58762024-11-0122111110.1186/s12967-024-05839-8Right ventricular volume overload reboots cardiomyocyte proliferation via immune responsesChunxia Zhou0Yuqing Hu1Zhuoya Dong2Zheng Wang3Sixie Zheng4Debao Li5Yingying Xiao6Dian Chen7Hao Chen8Sijuan Sun9Lincai Ye10Haibo Zhang11Department of Thoracic and Cardiovascular Surgery, Shanghai Children’s Medical Center, Shanghai Jiao Tong University School of MedicineDepartment of Cardiology, Shanghai Children’s Medical Center, Shanghai Jiao Tong University School of MedicineDepartment of Pediatric Intensive Care Unit, Ningbo Women and Children’s HospitalDepartment of Thoracic and Cardiovascular Surgery, Shanghai Children’s Medical Center, Shanghai Jiao Tong University School of MedicineDepartment of Thoracic and Cardiovascular Surgery, Shanghai Children’s Medical Center, Shanghai Jiao Tong University School of MedicineDepartment of Pediatric Surgery, Children’s Hospital of Fudan University, National Children’s Medical CenterDepartment of Thoracic and Cardiovascular Surgery, Shanghai Children’s Hospital, School of Medicine, Shanghai Jiao Tong UniversityDepartment of Thoracic and Cardiovascular Surgery, Shanghai Children’s Medical Center, Shanghai Jiao Tong University School of MedicineDepartment of Thoracic and Cardiovascular Surgery, Shanghai Children’s Medical Center, Shanghai Jiao Tong University School of MedicineDepartment of Pediatric Intensive Care Unit, Shanghai Children’s Medical Center, Shanghai Jiao Tong University School of MedicineDepartment of Thoracic and Cardiovascular Surgery, Shanghai Children’s Medical Center, Shanghai Jiao Tong University School of MedicineDepartment of Thoracic and Cardiovascular Surgery, Shanghai Children’s Medical Center, Shanghai Jiao Tong University School of MedicineAbstract Background Right ventricular volume overload (RVVO) is one of the most important hemodynamic characteristics in children with congenital heart disease (CHD) and heart failure, and cardiomyocyte (CM) proliferation is one of the most vital factors for improving cardiac performance. However, whether and how RVVO reboots CM proliferation remains elusive. Methods and results We first created a neonatal RVVO mouse model via abdominal aorta and inferior vena cava-fistula microsurgery at postnatal day 7 (P7), the edge of CM proliferation window. We subsequently performed bulk RNA-seq, single cell RNA-seq/flow cytometry, and immunofluorescence staining on the right ventricles (RV) of RVVO mice at P14/P21, defined as prepubertal stage, revealing that RVVO temporarily reboots prepubertal CM proliferation via immune responses. Conclusions In considering the importance of RVVO and CM proliferation, this study may bring an opportunity to create a novel paradigm to treat pediatric CHDs or heart failure. Graphical Abstracthttps://doi.org/10.1186/s12967-024-05839-8Volume overloadCardiomyocyteProliferationImmune responseCHD |
| spellingShingle | Chunxia Zhou Yuqing Hu Zhuoya Dong Zheng Wang Sixie Zheng Debao Li Yingying Xiao Dian Chen Hao Chen Sijuan Sun Lincai Ye Haibo Zhang Right ventricular volume overload reboots cardiomyocyte proliferation via immune responses Journal of Translational Medicine Volume overload Cardiomyocyte Proliferation Immune response CHD |
| title | Right ventricular volume overload reboots cardiomyocyte proliferation via immune responses |
| title_full | Right ventricular volume overload reboots cardiomyocyte proliferation via immune responses |
| title_fullStr | Right ventricular volume overload reboots cardiomyocyte proliferation via immune responses |
| title_full_unstemmed | Right ventricular volume overload reboots cardiomyocyte proliferation via immune responses |
| title_short | Right ventricular volume overload reboots cardiomyocyte proliferation via immune responses |
| title_sort | right ventricular volume overload reboots cardiomyocyte proliferation via immune responses |
| topic | Volume overload Cardiomyocyte Proliferation Immune response CHD |
| url | https://doi.org/10.1186/s12967-024-05839-8 |
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