Long-term cellular immunity of vaccines for Zaire Ebola Virus Diseases

Abstract Recent Ebola outbreaks underscore the importance of continuous prevention and disease control efforts. Authorized vaccines include Merck’s Ervebo (rVSV-ZEBOV) and Johnson & Johnson’s two-dose combination (Ad26.ZEBOV/MVA-BN-Filo). Here, in a five-year follow-up of the PREVAC randomized t...

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Main Authors: Aurélie Wiedemann, Edouard Lhomme, Mélanie Huchon, Emile Foucat, Marion Bérerd-Camara, Lydia Guillaumat, Marcel Yaradouno, Jacqueline Tambalou, Cécile Rodrigues, Alexandre Ribeiro, Abdoul Habib Béavogui, Christine Lacabaratz, Rodolphe Thiébaut, Laura Richert, Yves Lévy, the Prevac study team
Format: Article
Language:English
Published: Nature Portfolio 2024-09-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-024-51453-z
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author Aurélie Wiedemann
Edouard Lhomme
Mélanie Huchon
Emile Foucat
Marion Bérerd-Camara
Lydia Guillaumat
Marcel Yaradouno
Jacqueline Tambalou
Cécile Rodrigues
Alexandre Ribeiro
Abdoul Habib Béavogui
Christine Lacabaratz
Rodolphe Thiébaut
Laura Richert
Yves Lévy
the Prevac study team
author_facet Aurélie Wiedemann
Edouard Lhomme
Mélanie Huchon
Emile Foucat
Marion Bérerd-Camara
Lydia Guillaumat
Marcel Yaradouno
Jacqueline Tambalou
Cécile Rodrigues
Alexandre Ribeiro
Abdoul Habib Béavogui
Christine Lacabaratz
Rodolphe Thiébaut
Laura Richert
Yves Lévy
the Prevac study team
author_sort Aurélie Wiedemann
collection DOAJ
description Abstract Recent Ebola outbreaks underscore the importance of continuous prevention and disease control efforts. Authorized vaccines include Merck’s Ervebo (rVSV-ZEBOV) and Johnson & Johnson’s two-dose combination (Ad26.ZEBOV/MVA-BN-Filo). Here, in a five-year follow-up of the PREVAC randomized trial (NCT02876328), we report the results of the immunology ancillary study of the trial. The primary endpoint is to evaluate long-term memory T-cell responses induced by three vaccine regimens: Ad26–MVA, rVSV, and rVSV–booster. Polyfunctional EBOV-specific CD4+ T-cell responses increase after Ad26 priming and are further boosted by MVA, whereas minimal responses are observed in the rVSV groups, declining after one year. In-vitro expansion for eight days show sustained EBOV-specific T-cell responses for up to 60 months post-prime vaccination with both Ad26-MVA and rVSV, with no decline. Cytokine production analysis identify shared biomarkers between the Ad26-MVA and rVSV groups. In secondary endpoint, we observed an elevation of pro-inflammatory cytokines at Day 7 in the rVSV group. Finally, we establish a correlation between EBOV-specific T-cell responses and anti-EBOV IgG responses. Our findings can guide booster vaccination recommendations and help identify populations likely to benefit from revaccination.
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spelling doaj-art-8cb4eec69ef44d87a3425cc6b54f12802025-08-20T04:02:57ZengNature PortfolioNature Communications2041-17232024-09-0115111510.1038/s41467-024-51453-zLong-term cellular immunity of vaccines for Zaire Ebola Virus DiseasesAurélie Wiedemann0Edouard Lhomme1Mélanie Huchon2Emile Foucat3Marion Bérerd-Camara4Lydia Guillaumat5Marcel Yaradouno6Jacqueline Tambalou7Cécile Rodrigues8Alexandre Ribeiro9Abdoul Habib Béavogui10Christine Lacabaratz11Rodolphe Thiébaut12Laura Richert13Yves Lévy14the Prevac study teamVaccine Research Institute, Université Paris-EstVaccine Research Institute, Université Paris-EstVaccine Research Institute, Université Paris-EstVaccine Research Institute, Université Paris-EstAlliance for International Medical ActionVaccine Research Institute, Université Paris-EstAlliance for International Medical ActionAlliance for International Medical ActionVaccine Research Institute, Université Paris-EstVaccine Research Institute, Université Paris-EstCentre National de Formation et de Recherche en Santé Rurale (CNFRSR)Vaccine Research Institute, Université Paris-EstVaccine Research Institute, Université Paris-EstVaccine Research Institute, Université Paris-EstVaccine Research Institute, Université Paris-EstAbstract Recent Ebola outbreaks underscore the importance of continuous prevention and disease control efforts. Authorized vaccines include Merck’s Ervebo (rVSV-ZEBOV) and Johnson & Johnson’s two-dose combination (Ad26.ZEBOV/MVA-BN-Filo). Here, in a five-year follow-up of the PREVAC randomized trial (NCT02876328), we report the results of the immunology ancillary study of the trial. The primary endpoint is to evaluate long-term memory T-cell responses induced by three vaccine regimens: Ad26–MVA, rVSV, and rVSV–booster. Polyfunctional EBOV-specific CD4+ T-cell responses increase after Ad26 priming and are further boosted by MVA, whereas minimal responses are observed in the rVSV groups, declining after one year. In-vitro expansion for eight days show sustained EBOV-specific T-cell responses for up to 60 months post-prime vaccination with both Ad26-MVA and rVSV, with no decline. Cytokine production analysis identify shared biomarkers between the Ad26-MVA and rVSV groups. In secondary endpoint, we observed an elevation of pro-inflammatory cytokines at Day 7 in the rVSV group. Finally, we establish a correlation between EBOV-specific T-cell responses and anti-EBOV IgG responses. Our findings can guide booster vaccination recommendations and help identify populations likely to benefit from revaccination.https://doi.org/10.1038/s41467-024-51453-z
spellingShingle Aurélie Wiedemann
Edouard Lhomme
Mélanie Huchon
Emile Foucat
Marion Bérerd-Camara
Lydia Guillaumat
Marcel Yaradouno
Jacqueline Tambalou
Cécile Rodrigues
Alexandre Ribeiro
Abdoul Habib Béavogui
Christine Lacabaratz
Rodolphe Thiébaut
Laura Richert
Yves Lévy
the Prevac study team
Long-term cellular immunity of vaccines for Zaire Ebola Virus Diseases
Nature Communications
title Long-term cellular immunity of vaccines for Zaire Ebola Virus Diseases
title_full Long-term cellular immunity of vaccines for Zaire Ebola Virus Diseases
title_fullStr Long-term cellular immunity of vaccines for Zaire Ebola Virus Diseases
title_full_unstemmed Long-term cellular immunity of vaccines for Zaire Ebola Virus Diseases
title_short Long-term cellular immunity of vaccines for Zaire Ebola Virus Diseases
title_sort long term cellular immunity of vaccines for zaire ebola virus diseases
url https://doi.org/10.1038/s41467-024-51453-z
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