Evaluation the tissue inhibitor of matrix metalloproteinases first type concentration in various platelet-related products
Purpose: to evaluate the content of tissue inhibitor of matrix metalloproteinase type 1 (TIMP-1) in platelet preparations obtained in different methods.Material and methods. Platelet-rich plasma (PRP), platelet-poor plasma, and a suspension of platelets washed from plasma (WP) were isolated from the...
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| Format: | Article |
| Language: | Russian |
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Real Time Ltd
2024-12-01
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| Series: | Российский офтальмологический журнал |
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| Online Access: | https://roj.igb.ru/jour/article/view/1627 |
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| author | E. V. Chentsova N. V. Borovkova K. V. Sirotkina O. V. Beznos M. S. Makarov I. N. Ponomarev M. V. Storozheva |
| author_facet | E. V. Chentsova N. V. Borovkova K. V. Sirotkina O. V. Beznos M. S. Makarov I. N. Ponomarev M. V. Storozheva |
| author_sort | E. V. Chentsova |
| collection | DOAJ |
| description | Purpose: to evaluate the content of tissue inhibitor of matrix metalloproteinase type 1 (TIMP-1) in platelet preparations obtained in different methods.Material and methods. Platelet-rich plasma (PRP), platelet-poor plasma, and a suspension of platelets washed from plasma (WP) were isolated from the blood of 10 volunteer donors, and a morphofunctional analysis of platelets was performed. After that, a platelet lysate was prepared from PRP, WP, and platelet-poor plasma. The concentration of TIMP-1 in the lysates was determined using enzyme immunoassay.Results. The level of TIMP-1 in the lysates of PRP and WP did not differ significantly; in platelet-poor plasma, the level of TIMP-1 was significantly lower than in the lysates of PRP and WP (p = 0.003). In all types of preparations, the concentration of TIMP-1 was 2-4 times higher than similar values in the blood serum of healthy people, as reported in other studies. A weak correlation was found between the TIMP-1 concentration and the platelet number in PRP, and there was no correlation between the content of platelets with granules and the TIMP-1 level in the lysates. The presence of leukocytes in the initial PRP also did not affect the TIMP level in the final lysates. A strong correlation was found between the TIMP-1 concentration in the WP lysate and in platelet-poor plasma, as well as a direct correlation between the total platelet content and the TIMP-1 level in the PRP lysate.Conclusions. Preparations with high concentrations of TIMP-1 can be obtained both from concentrated platelet suspensions and from platelet-poor plasma. To optimize the method, it is necessary to study the effect of platelet preparations with TIMP on various biological models. |
| format | Article |
| id | doaj-art-8cb048551f4d4973ba539feffddede7a |
| institution | Kabale University |
| issn | 2072-0076 2587-5760 |
| language | Russian |
| publishDate | 2024-12-01 |
| publisher | Real Time Ltd |
| record_format | Article |
| series | Российский офтальмологический журнал |
| spelling | doaj-art-8cb048551f4d4973ba539feffddede7a2025-08-20T03:59:20ZrusReal Time LtdРоссийский офтальмологический журнал2072-00762587-57602024-12-01174848810.21516/2072-0076-2024-17-4-84-88741Evaluation the tissue inhibitor of matrix metalloproteinases first type concentration in various platelet-related productsE. V. Chentsova0N. V. Borovkova1K. V. Sirotkina2O. V. Beznos3M. S. Makarov4I. N. Ponomarev5M. V. Storozheva6Helmholtz National Medical Research Center of Eye DiseasesN.V. Sklifosovsky Research Institute for Emergency Medicine; Pirogov Russian National Research Medical UniversityHelmholtz National Medical Research Center of Eye DiseasesHelmholtz National Medical Research Center of Eye DiseasesN.V. Sklifosovsky Research Institute for Emergency MedicineN.V. Sklifosovsky Research Institute for Emergency MedicineN.V. Sklifosovsky Research Institute for Emergency MedicinePurpose: to evaluate the content of tissue inhibitor of matrix metalloproteinase type 1 (TIMP-1) in platelet preparations obtained in different methods.Material and methods. Platelet-rich plasma (PRP), platelet-poor plasma, and a suspension of platelets washed from plasma (WP) were isolated from the blood of 10 volunteer donors, and a morphofunctional analysis of platelets was performed. After that, a platelet lysate was prepared from PRP, WP, and platelet-poor plasma. The concentration of TIMP-1 in the lysates was determined using enzyme immunoassay.Results. The level of TIMP-1 in the lysates of PRP and WP did not differ significantly; in platelet-poor plasma, the level of TIMP-1 was significantly lower than in the lysates of PRP and WP (p = 0.003). In all types of preparations, the concentration of TIMP-1 was 2-4 times higher than similar values in the blood serum of healthy people, as reported in other studies. A weak correlation was found between the TIMP-1 concentration and the platelet number in PRP, and there was no correlation between the content of platelets with granules and the TIMP-1 level in the lysates. The presence of leukocytes in the initial PRP also did not affect the TIMP level in the final lysates. A strong correlation was found between the TIMP-1 concentration in the WP lysate and in platelet-poor plasma, as well as a direct correlation between the total platelet content and the TIMP-1 level in the PRP lysate.Conclusions. Preparations with high concentrations of TIMP-1 can be obtained both from concentrated platelet suspensions and from platelet-poor plasma. To optimize the method, it is necessary to study the effect of platelet preparations with TIMP on various biological models.https://roj.igb.ru/jour/article/view/1627keratolysiscorneaplateletsgranuleslysatetissue inhibitor of metalloproteinases |
| spellingShingle | E. V. Chentsova N. V. Borovkova K. V. Sirotkina O. V. Beznos M. S. Makarov I. N. Ponomarev M. V. Storozheva Evaluation the tissue inhibitor of matrix metalloproteinases first type concentration in various platelet-related products Российский офтальмологический журнал keratolysis cornea platelets granules lysate tissue inhibitor of metalloproteinases |
| title | Evaluation the tissue inhibitor of matrix metalloproteinases first type concentration in various platelet-related products |
| title_full | Evaluation the tissue inhibitor of matrix metalloproteinases first type concentration in various platelet-related products |
| title_fullStr | Evaluation the tissue inhibitor of matrix metalloproteinases first type concentration in various platelet-related products |
| title_full_unstemmed | Evaluation the tissue inhibitor of matrix metalloproteinases first type concentration in various platelet-related products |
| title_short | Evaluation the tissue inhibitor of matrix metalloproteinases first type concentration in various platelet-related products |
| title_sort | evaluation the tissue inhibitor of matrix metalloproteinases first type concentration in various platelet related products |
| topic | keratolysis cornea platelets granules lysate tissue inhibitor of metalloproteinases |
| url | https://roj.igb.ru/jour/article/view/1627 |
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