Stability Indicating RP-HPLC Method for Determination of Valsartan in Pure and Pharmaceutical Formulation
A simple, rapid and accurate and stability indicating RP-HPLC method was developed for the determination of valsartan in pure and tablet forms. The method showed a linear response for concentrations in the range of 50-175 µg/mL using 0.01 M NH4H2PO4 (pH 3.5) buffer: methanol [50:50] as the mobile ph...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | E-Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2010/487197 |
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| author | S. K. Patro S. K. Kanungo V. J. Patro N. S. K. Choudhury |
| author_facet | S. K. Patro S. K. Kanungo V. J. Patro N. S. K. Choudhury |
| author_sort | S. K. Patro |
| collection | DOAJ |
| description | A simple, rapid and accurate and stability indicating RP-HPLC method was developed for the determination of valsartan in pure and tablet forms. The method showed a linear response for concentrations in the range of 50-175 µg/mL using 0.01 M NH4H2PO4 (pH 3.5) buffer: methanol [50:50] as the mobile phase with detection at 210 nm and a flow rate of 1 mL/min and retention time 11.041 min. The method was statistically validated for accuracy, precision, linearity, ruggedness, robustness, forced degradation, solution stability and selectivity. Quantitative and recovery studies of the dosage form were also carried out and analyzed; the % RSD from recovery studies was found to be less than 1. Due to simplicity, rapidity and accuracy of the method, we believe that the method will be useful for routine quality control analysis. |
| format | Article |
| id | doaj-art-8ca49fb5790f4bafa5ba86f5efdbddd9 |
| institution | Kabale University |
| issn | 0973-4945 2090-9810 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | E-Journal of Chemistry |
| spelling | doaj-art-8ca49fb5790f4bafa5ba86f5efdbddd92025-08-20T03:37:44ZengWileyE-Journal of Chemistry0973-49452090-98102010-01-017124625210.1155/2010/487197Stability Indicating RP-HPLC Method for Determination of Valsartan in Pure and Pharmaceutical FormulationS. K. Patro0S. K. Kanungo1V. J. Patro2N. S. K. Choudhury3Department of Pharmaceutical Analysis & Quality Assurance, Institute of Pharmacy and Technology, Salipur, Cuttack, Orissa-754202, IndiaDepartment of Pharmaceutical Analysis & Quality Assurance, Institute of Pharmacy and Technology, Salipur, Cuttack, Orissa-754202, IndiaCollege of Pharmaceutical Sciences, Mohuda, Berhampur, Ganjam, Orissa, IndiaDepartment of Pharmacy, S. C.B. Medical College, Cuttack, Orissa, IndiaA simple, rapid and accurate and stability indicating RP-HPLC method was developed for the determination of valsartan in pure and tablet forms. The method showed a linear response for concentrations in the range of 50-175 µg/mL using 0.01 M NH4H2PO4 (pH 3.5) buffer: methanol [50:50] as the mobile phase with detection at 210 nm and a flow rate of 1 mL/min and retention time 11.041 min. The method was statistically validated for accuracy, precision, linearity, ruggedness, robustness, forced degradation, solution stability and selectivity. Quantitative and recovery studies of the dosage form were also carried out and analyzed; the % RSD from recovery studies was found to be less than 1. Due to simplicity, rapidity and accuracy of the method, we believe that the method will be useful for routine quality control analysis.http://dx.doi.org/10.1155/2010/487197 |
| spellingShingle | S. K. Patro S. K. Kanungo V. J. Patro N. S. K. Choudhury Stability Indicating RP-HPLC Method for Determination of Valsartan in Pure and Pharmaceutical Formulation E-Journal of Chemistry |
| title | Stability Indicating RP-HPLC Method for Determination of Valsartan in Pure and Pharmaceutical Formulation |
| title_full | Stability Indicating RP-HPLC Method for Determination of Valsartan in Pure and Pharmaceutical Formulation |
| title_fullStr | Stability Indicating RP-HPLC Method for Determination of Valsartan in Pure and Pharmaceutical Formulation |
| title_full_unstemmed | Stability Indicating RP-HPLC Method for Determination of Valsartan in Pure and Pharmaceutical Formulation |
| title_short | Stability Indicating RP-HPLC Method for Determination of Valsartan in Pure and Pharmaceutical Formulation |
| title_sort | stability indicating rp hplc method for determination of valsartan in pure and pharmaceutical formulation |
| url | http://dx.doi.org/10.1155/2010/487197 |
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