Optimising Fungal Spore Cultivation and Inoculation Methods for Enhanced Antifungal Performance Testing of Textiles
To improve the precision and operability of antifungal performance testing, this study systematically examines the cultivation time, spore suspension preparation, and inoculation protocols for fungal spores commonly used in mildew and rot resistance testing. Key optimisations were achieved in inocul...
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| Format: | Article |
| Language: | English |
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2025-01-01
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| Series: | Textile & Leather Review |
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| Online Access: | https://www.tlr-journal.com/tlr-2024-166-deng/ |
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| _version_ | 1841525555579584512 |
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| author | Hongying Deng Chen Yang Zhenzhu Zhang Xiaoling Xu Qixuan Chen Yanan Zhang Xuefen He |
| author_facet | Hongying Deng Chen Yang Zhenzhu Zhang Xiaoling Xu Qixuan Chen Yanan Zhang Xuefen He |
| author_sort | Hongying Deng |
| collection | DOAJ |
| description | To improve the precision and operability of antifungal performance testing, this study systematically examines the cultivation time, spore suspension preparation, and inoculation protocols for fungal spores commonly used in mildew and rot resistance testing. Key optimisations were achieved in inoculum time, centrifugation conditions, and spray inoculation methods, enhancing experimental reliability. The findings indicate that Aspergillus brasiliensis and Trichoderma lignorum spores form abundantly within 5–7 days, while Chaetomium globosum requires 17–20 days. Penicillium funiculosum, Aureobasidium pullulans, Paecilomyces variotii, and Trichoderma virens reach sufficient spore production in 13–15 days. Effective spore separation was achieved via centrifugation at 6000 r/min for 10 minutes. Uniform and quantitative spore inoculation was ensured using 20–50 μm spray nozzles. Quantitative methods, including specific turbidity, hemocytometer counting, and the plate count method, were evaluated, with turbidity and hemocytometer methods offering efficiency and simplicity, while plate counts provided verification. These findings contribute to establishing standardised and reproducible antifungal testing procedures, addressing operational guidelines gaps and supporting the development of antifungal technologies. |
| format | Article |
| id | doaj-art-8c89fc4f8dee4c2cafd611967493defa |
| institution | Kabale University |
| issn | 2623-6281 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | idd3 |
| record_format | Article |
| series | Textile & Leather Review |
| spelling | doaj-art-8c89fc4f8dee4c2cafd611967493defa2025-01-17T09:16:44Zengidd3Textile & Leather Review2623-62812025-01-018193710.31881/TLR.2024.166Optimising Fungal Spore Cultivation and Inoculation Methods for Enhanced Antifungal Performance Testing of TextilesHongying DengChen YangZhenzhu ZhangXiaoling XuQixuan ChenYanan ZhangXuefen HeTo improve the precision and operability of antifungal performance testing, this study systematically examines the cultivation time, spore suspension preparation, and inoculation protocols for fungal spores commonly used in mildew and rot resistance testing. Key optimisations were achieved in inoculum time, centrifugation conditions, and spray inoculation methods, enhancing experimental reliability. The findings indicate that Aspergillus brasiliensis and Trichoderma lignorum spores form abundantly within 5–7 days, while Chaetomium globosum requires 17–20 days. Penicillium funiculosum, Aureobasidium pullulans, Paecilomyces variotii, and Trichoderma virens reach sufficient spore production in 13–15 days. Effective spore separation was achieved via centrifugation at 6000 r/min for 10 minutes. Uniform and quantitative spore inoculation was ensured using 20–50 μm spray nozzles. Quantitative methods, including specific turbidity, hemocytometer counting, and the plate count method, were evaluated, with turbidity and hemocytometer methods offering efficiency and simplicity, while plate counts provided verification. These findings contribute to establishing standardised and reproducible antifungal testing procedures, addressing operational guidelines gaps and supporting the development of antifungal technologies.https://www.tlr-journal.com/tlr-2024-166-deng/antifungal activityfungal spore preparationquantitative inoculationspore counting methods |
| spellingShingle | Hongying Deng Chen Yang Zhenzhu Zhang Xiaoling Xu Qixuan Chen Yanan Zhang Xuefen He Optimising Fungal Spore Cultivation and Inoculation Methods for Enhanced Antifungal Performance Testing of Textiles Textile & Leather Review antifungal activity fungal spore preparation quantitative inoculation spore counting methods |
| title | Optimising Fungal Spore Cultivation and Inoculation Methods for Enhanced Antifungal Performance Testing of Textiles |
| title_full | Optimising Fungal Spore Cultivation and Inoculation Methods for Enhanced Antifungal Performance Testing of Textiles |
| title_fullStr | Optimising Fungal Spore Cultivation and Inoculation Methods for Enhanced Antifungal Performance Testing of Textiles |
| title_full_unstemmed | Optimising Fungal Spore Cultivation and Inoculation Methods for Enhanced Antifungal Performance Testing of Textiles |
| title_short | Optimising Fungal Spore Cultivation and Inoculation Methods for Enhanced Antifungal Performance Testing of Textiles |
| title_sort | optimising fungal spore cultivation and inoculation methods for enhanced antifungal performance testing of textiles |
| topic | antifungal activity fungal spore preparation quantitative inoculation spore counting methods |
| url | https://www.tlr-journal.com/tlr-2024-166-deng/ |
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