The Influence of Diabetes Mellitus and Kidney Dysfunction on Oxidative Stress, a Reflection of the Multisystem Interactions in Aortic Stenosis
Progression of aortic stenosis (AS) is aggravated by type 2 Diabetes Mellitus (T2DM) and kidney dysfunction (KD). Oxidative stress is one of the main mechanisms that triggers AS and is also disturbed among subjects with T2DM and KD. Consequently, we studied the redox homeostasis in four groups of pa...
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MDPI AG
2025-07-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/14/7/888 |
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| author | Laura Mourino-Alvarez Inés Perales-Sánchez Germán Hernández-Fernández Gabriel Blanco-López Emilio Blanco-López Rocío Eiros Cristian Herrera-Flores Miryam González-Cebrian Teresa Tejerina Jesús Piqueras-Flores Pedro Luis Sánchez Luis F. López-Almodóvar Luis R. Padial Maria G. Barderas |
| author_facet | Laura Mourino-Alvarez Inés Perales-Sánchez Germán Hernández-Fernández Gabriel Blanco-López Emilio Blanco-López Rocío Eiros Cristian Herrera-Flores Miryam González-Cebrian Teresa Tejerina Jesús Piqueras-Flores Pedro Luis Sánchez Luis F. López-Almodóvar Luis R. Padial Maria G. Barderas |
| author_sort | Laura Mourino-Alvarez |
| collection | DOAJ |
| description | Progression of aortic stenosis (AS) is aggravated by type 2 Diabetes Mellitus (T2DM) and kidney dysfunction (KD). Oxidative stress is one of the main mechanisms that triggers AS and is also disturbed among subjects with T2DM and KD. Consequently, we studied the redox homeostasis in four groups of patients, also classifying each patient based on their kidney function: control subjects, T2DM, AS, and AS+T2DM. Free reduced thiols in plasma were analyzed using a colorimetric assay, and the redox state of human serum albumin (HSA) was assessed by immunodetection and PEG-PCMal labeling. Lower levels of thiols were evident in patients with AS and AS+T2DM, while reduced and mildly oxidized HSA was more abundant in T2DM and AS+T2DM patients, reflecting less protection against oxidation. Moreover, the thiol levels decreased as KD increased in patients with AS and AS+T2DM. Differences also exist in reduced and mildly oxidized HSA between patients with normal and severely impaired kidney function, whereas AS patients with severe KD had more strongly oxidized HSA. Our results confirm an imbalance in oxidative stress associated with AS that is aggravated by the coexistence of T2DM and KD. Moreover, T2DM treatment might mitigate this dysfunction, opening the door to new therapeutic approaches for these patients. |
| format | Article |
| id | doaj-art-8c7471e080fb4d47832b852f2e06223f |
| institution | Kabale University |
| issn | 2076-3921 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj-art-8c7471e080fb4d47832b852f2e06223f2025-08-20T03:58:30ZengMDPI AGAntioxidants2076-39212025-07-0114788810.3390/antiox14070888The Influence of Diabetes Mellitus and Kidney Dysfunction on Oxidative Stress, a Reflection of the Multisystem Interactions in Aortic StenosisLaura Mourino-Alvarez0Inés Perales-Sánchez1Germán Hernández-Fernández2Gabriel Blanco-López3Emilio Blanco-López4Rocío Eiros5Cristian Herrera-Flores6Miryam González-Cebrian7Teresa Tejerina8Jesús Piqueras-Flores9Pedro Luis Sánchez10Luis F. López-Almodóvar11Luis R. Padial12Maria G. Barderas13Department of Vascular Physiopathology, Hospital Nacional de Parapléjicos, SESCAM, 45071 Toledo, SpainDepartment of Vascular Physiopathology, Hospital Nacional de Parapléjicos, SESCAM, 45071 Toledo, SpainDepartment of Vascular Physiopathology, Hospital Nacional de Parapléjicos, SESCAM, 45071 Toledo, SpainDepartment of Vascular Physiopathology, Hospital Nacional de Parapléjicos, SESCAM, 45071 Toledo, SpainDepartment of Vascular Physiopathology, Hospital Nacional de Parapléjicos, IDISCAM, 45071 Toledo, SpainDepartment of Cardiology, Hospital Universitario de Salamanca, Instituto de Investigación Biomédica de Salamanca, Centro de Investigación Biomédica en Red de Enfermedades Cardiovasculares (CIBER-CV), 37007 Salamanca, SpainDepartment of Cardiology, Hospital Universitario de Salamanca, Instituto de Investigación Biomédica de Salamanca, Centro de Investigación Biomédica en Red de Enfermedades Cardiovasculares (CIBER-CV), 37007 Salamanca, SpainDepartment of Cardiology, Hospital Universitario de Salamanca, Instituto de Investigación Biomédica de Salamanca, Centro de Investigación Biomédica en Red de Enfermedades Cardiovasculares (CIBER-CV), 37007 Salamanca, SpainDepartment of Pharmacology, School of Medicine, Universidad Complutense de Madrid, 28040 Madrid, SpainHeart Failure and Inherited Cardiovascular Diseases Unit, Ciudad Real General University Hospital, 13005 Ciudad Real, SpainDepartment of Cardiology, Hospital Universitario de Salamanca, Instituto de Investigación Biomédica de Salamanca, Centro de Investigación Biomédica en Red de Enfermedades Cardiovasculares (CIBER-CV), 37007 Salamanca, SpainCardiac Surgery, Hospital General Universitario de Toledo, SESCAM, 45007 Toledo, SpainDepartment of Cardiology, Hospital General Universitario de Toledo, SESCAM, 45007 Toledo, SpainDepartment of Vascular Physiopathology, Hospital Nacional de Parapléjicos, SESCAM, 45071 Toledo, SpainProgression of aortic stenosis (AS) is aggravated by type 2 Diabetes Mellitus (T2DM) and kidney dysfunction (KD). Oxidative stress is one of the main mechanisms that triggers AS and is also disturbed among subjects with T2DM and KD. Consequently, we studied the redox homeostasis in four groups of patients, also classifying each patient based on their kidney function: control subjects, T2DM, AS, and AS+T2DM. Free reduced thiols in plasma were analyzed using a colorimetric assay, and the redox state of human serum albumin (HSA) was assessed by immunodetection and PEG-PCMal labeling. Lower levels of thiols were evident in patients with AS and AS+T2DM, while reduced and mildly oxidized HSA was more abundant in T2DM and AS+T2DM patients, reflecting less protection against oxidation. Moreover, the thiol levels decreased as KD increased in patients with AS and AS+T2DM. Differences also exist in reduced and mildly oxidized HSA between patients with normal and severely impaired kidney function, whereas AS patients with severe KD had more strongly oxidized HSA. Our results confirm an imbalance in oxidative stress associated with AS that is aggravated by the coexistence of T2DM and KD. Moreover, T2DM treatment might mitigate this dysfunction, opening the door to new therapeutic approaches for these patients.https://www.mdpi.com/2076-3921/14/7/888aortic stenosiskidney dysfunctiondiabetes mellitusoxidative stressthiolsalbumin |
| spellingShingle | Laura Mourino-Alvarez Inés Perales-Sánchez Germán Hernández-Fernández Gabriel Blanco-López Emilio Blanco-López Rocío Eiros Cristian Herrera-Flores Miryam González-Cebrian Teresa Tejerina Jesús Piqueras-Flores Pedro Luis Sánchez Luis F. López-Almodóvar Luis R. Padial Maria G. Barderas The Influence of Diabetes Mellitus and Kidney Dysfunction on Oxidative Stress, a Reflection of the Multisystem Interactions in Aortic Stenosis Antioxidants aortic stenosis kidney dysfunction diabetes mellitus oxidative stress thiols albumin |
| title | The Influence of Diabetes Mellitus and Kidney Dysfunction on Oxidative Stress, a Reflection of the Multisystem Interactions in Aortic Stenosis |
| title_full | The Influence of Diabetes Mellitus and Kidney Dysfunction on Oxidative Stress, a Reflection of the Multisystem Interactions in Aortic Stenosis |
| title_fullStr | The Influence of Diabetes Mellitus and Kidney Dysfunction on Oxidative Stress, a Reflection of the Multisystem Interactions in Aortic Stenosis |
| title_full_unstemmed | The Influence of Diabetes Mellitus and Kidney Dysfunction on Oxidative Stress, a Reflection of the Multisystem Interactions in Aortic Stenosis |
| title_short | The Influence of Diabetes Mellitus and Kidney Dysfunction on Oxidative Stress, a Reflection of the Multisystem Interactions in Aortic Stenosis |
| title_sort | influence of diabetes mellitus and kidney dysfunction on oxidative stress a reflection of the multisystem interactions in aortic stenosis |
| topic | aortic stenosis kidney dysfunction diabetes mellitus oxidative stress thiols albumin |
| url | https://www.mdpi.com/2076-3921/14/7/888 |
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