Characteristics of an endogenous compound microbial inoculant and its immobilization effect on wastewater treatment from pig feedlots
Taking the wastewater from large-scale pig feedlots as the research object and the original wastewater as the main nutrient source, an endogenous compound microbial inoculant was successfully prepared by the enrichment method, which was named as WKM, and its removal effects on chemical oxygen demand...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2021-01-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2020.03.122 |
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| Summary: | Taking the wastewater from large-scale pig feedlots as the research object and the original wastewater as the main nutrient source, an endogenous compound microbial inoculant was successfully prepared by the enrichment method, which was named as WKM, and its removal effects on chemical oxygen demand (COD) and ammonia nitrogen in the process of wastewater treatment from pig feedlots were analyzed and monitored. The results showed that the removal rates of COD and ammonia nitrogen in the pig feedlot wastewater (ZW) were 94.8% and 61.8%, respectively, after 3 d by adding the WKM. The high-throughput sequencing results indicated that the main microbial species of WKM belonged to Proteobacteria, Actinobacteria and Bacteroidetes at the phylum level. The main microbial species at the genus level belonged to Leucobacter, Castellaniella, Camelimonas, Moheibacter, Nitrosomonas, Cloacibacillus and Pusillimonas, of which the mass ratio was approximately 2∶2∶2∶2∶1.5∶1.5∶1. The results based on the clusters of orthologous groups of proteins (COG) analysis showed the abundances of amino acid transport and metabolism ([E]), energy production and conversion ([C]), carbohydrate transport and metabolism ([G]), inorganic ion transport and metabolism ([P]) and coenzyme transport and metabolism ([H]) were relatively high, which played an essential role in the conversion of organic substances such as proteins and lipids in the pig feedlot wastewater containing high concentrations of ammonia nitrogen and organic pollutants. Furthermore, the endogenous compound microbial inoculant immobilized with wheat bran showed a higher removal efficiency for COD in the pig feedlot wastewater (ZR), compared with the non-addition group (P＜0.01) and WKM group (P＜0.05). Besides, one-way analysis of variance showed that the immobilization ratio of 1∶50 (mass/volume) of wheat bran to compound microbial inoculant had the highest removal efficiency in COD (P＜0.05) among all the treatments, reaching to 89.4% in the pig feedlot wastewater (ZR). To sum up, the endogenous compound microbial inoculant immobilized with wheat bran has potential application prospect and practical value at wastewater treatment from pig feedlots. |
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| ISSN: | 1008-9209 2097-5155 |