Phytochemical analysis and neuroprotective potential of Achillea santolina L. fractions
Abstract Phytochemical characterization, and biological assessment of the neuroprotective activities of Achillea santolina L. methanolic extract (AS), its methylene chloride fraction (MF), butanol fraction (BF) and their isolated compounds were investigated. Twenty-two compounds were identified and...
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Nature Portfolio
2025-05-01
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| author | Passent M. Abdel-Baki Nariman E. Mahdy Rana M. Ibrahim Shymaa A. El Badawy Sara E. Ali Marwa A. Ibrahim Marwa S. Khattab Ahmed A. El-Rashedy Shimaa R. Emam |
| author_facet | Passent M. Abdel-Baki Nariman E. Mahdy Rana M. Ibrahim Shymaa A. El Badawy Sara E. Ali Marwa A. Ibrahim Marwa S. Khattab Ahmed A. El-Rashedy Shimaa R. Emam |
| author_sort | Passent M. Abdel-Baki |
| collection | DOAJ |
| description | Abstract Phytochemical characterization, and biological assessment of the neuroprotective activities of Achillea santolina L. methanolic extract (AS), its methylene chloride fraction (MF), butanol fraction (BF) and their isolated compounds were investigated. Twenty-two compounds were identified and quantified in AS using HPLC. Luteolin and kaempferol were isolated from MF. Isovitexin and kaempferol 3-O-glucoside were isolated from BF. The anti-inflammatory activity of the isolated compounds was determined by employing cyclooxygenase-2 (COX-2) and 5-lipoxygenase (5-LOX) inhibitory assays. Neuroprotective activities of AS, MF and BF were investigated against monosodium glutamate (MSG) induced neurotoxicity in rats. Brain damage amended significantly as indicated by the decreased lactate dehydrogenase and tumor necrosis factor. Brain oxidative status was restored as indicated by increased glutathione and decreased lipid peroxidation. AS, MF and BF markedly attenuated histopathological alterations in cerebral cortex and downregulated expression of COX-2, IL-1B, IL-10 genes. The affinity of the isolated compounds for Human Heme Oxygenase-1, 5-LOX, Kelch-like ECH-associated protein and COX-2 was studied. Molecular dynamics simulation and ADME study proved that isovitexin has stable conformations and binding patterns with the active sites of the studied enzymes. For the first time, the neuroprotective potential of AS, fractions and isolated compounds was determined supported by anti-inflammatory study. |
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| language | English |
| publishDate | 2025-05-01 |
| publisher | Nature Portfolio |
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| spelling | doaj-art-8c6b0f30a749491daefdaeb5cc2dac772025-08-20T01:49:33ZengNature PortfolioScientific Reports2045-23222025-05-0115111910.1038/s41598-025-98887-zPhytochemical analysis and neuroprotective potential of Achillea santolina L. fractionsPassent M. Abdel-Baki0Nariman E. Mahdy1Rana M. Ibrahim2Shymaa A. El Badawy3Sara E. Ali4Marwa A. Ibrahim5Marwa S. Khattab6Ahmed A. El-Rashedy7Shimaa R. Emam8Department of Pharmacognosy, Faculty of Pharmacy, Cairo UniversityDepartment of Pharmacognosy, Faculty of Pharmacy, Cairo UniversityDepartment of Pharmacognosy, Faculty of Pharmacy, Cairo UniversityDepartment of Pharmacology, Faculty of Veterinary Medicine, Cairo UniversityDepartment of Physiology, Faculty of Veterinary Medicine, Cairo UniversityDepartment of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Cairo UniversityDepartment of Pathology, Faculty of Veterinary Medicine, Cairo UniversityChemistry of Natural and Microbial Products Department, National Research Center (NRC)Department of Pharmacology, Faculty of Veterinary Medicine, Cairo UniversityAbstract Phytochemical characterization, and biological assessment of the neuroprotective activities of Achillea santolina L. methanolic extract (AS), its methylene chloride fraction (MF), butanol fraction (BF) and their isolated compounds were investigated. Twenty-two compounds were identified and quantified in AS using HPLC. Luteolin and kaempferol were isolated from MF. Isovitexin and kaempferol 3-O-glucoside were isolated from BF. The anti-inflammatory activity of the isolated compounds was determined by employing cyclooxygenase-2 (COX-2) and 5-lipoxygenase (5-LOX) inhibitory assays. Neuroprotective activities of AS, MF and BF were investigated against monosodium glutamate (MSG) induced neurotoxicity in rats. Brain damage amended significantly as indicated by the decreased lactate dehydrogenase and tumor necrosis factor. Brain oxidative status was restored as indicated by increased glutathione and decreased lipid peroxidation. AS, MF and BF markedly attenuated histopathological alterations in cerebral cortex and downregulated expression of COX-2, IL-1B, IL-10 genes. The affinity of the isolated compounds for Human Heme Oxygenase-1, 5-LOX, Kelch-like ECH-associated protein and COX-2 was studied. Molecular dynamics simulation and ADME study proved that isovitexin has stable conformations and binding patterns with the active sites of the studied enzymes. For the first time, the neuroprotective potential of AS, fractions and isolated compounds was determined supported by anti-inflammatory study.https://doi.org/10.1038/s41598-025-98887-zAchillea santolinaHPLCDockingInflammationMonosodium glutamateADME |
| spellingShingle | Passent M. Abdel-Baki Nariman E. Mahdy Rana M. Ibrahim Shymaa A. El Badawy Sara E. Ali Marwa A. Ibrahim Marwa S. Khattab Ahmed A. El-Rashedy Shimaa R. Emam Phytochemical analysis and neuroprotective potential of Achillea santolina L. fractions Scientific Reports Achillea santolina HPLC Docking Inflammation Monosodium glutamate ADME |
| title | Phytochemical analysis and neuroprotective potential of Achillea santolina L. fractions |
| title_full | Phytochemical analysis and neuroprotective potential of Achillea santolina L. fractions |
| title_fullStr | Phytochemical analysis and neuroprotective potential of Achillea santolina L. fractions |
| title_full_unstemmed | Phytochemical analysis and neuroprotective potential of Achillea santolina L. fractions |
| title_short | Phytochemical analysis and neuroprotective potential of Achillea santolina L. fractions |
| title_sort | phytochemical analysis and neuroprotective potential of achillea santolina l fractions |
| topic | Achillea santolina HPLC Docking Inflammation Monosodium glutamate ADME |
| url | https://doi.org/10.1038/s41598-025-98887-z |
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