Effects of Thifluzamide Treatment on the Production of Cell Wall Degrading Enzymes in <i>Rhizoctonia solani</i> and Phenylpropane Metabolism in Pear Fruit

Effects of Thifluzamide Treatment on the Production of Cell Wall Degrading Enzymes in <i>Rhizoctonia solani</i> and Phenylpropane Metabolism in Pear Fruit

The study aimed to investigate the effects of thifluzamide (2.67 mg/L) on ‘Huangguan’ pear fruit rot caused by <i>Rhizoctonia solani</i> during storage, as well as the activities of polygalacturonase (PG), pectin methylesterase (PME), polygalacturonic acid trans-eliminase (PGTE), pectin...

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Bibliographic Details
Main Authors: Yushuo Wu, Weiwei Yan, Xiaonan Sun, Xinnan Zhang, Yonghong Ge, Xiaohui Jia
Format: Article
Language:English
Published: MDPI AG 2024-11-01
Series:Pathogens
Subjects:
<i>Rhizoctonia solani</i>
thifluzamide
pear fruit
cell wall-degrading enzyme
phenylpropanoid pathway
Online Access:https://www.mdpi.com/2076-0817/13/11/963
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Summary:The study aimed to investigate the effects of thifluzamide (2.67 mg/L) on ‘Huangguan’ pear fruit rot caused by <i>Rhizoctonia solani</i> during storage, as well as the activities of polygalacturonase (PG), pectin methylesterase (PME), polygalacturonic acid trans-eliminase (PGTE), pectin methyl trans-eliminase (PMTE), xylanase, and pectate lyase (PL) secreted by <i>R. solani</i>. The results showed that thifluzamide treatment significantly inhibited the activities of PG, PME, PGTE, PMTE, xylanase, and PL secreted by <i>R. solani</i> after 3 days in vitro culture, compared to the control. Thifluzamide also increased the activities of phenylalanine ammonia-lyase (PAL), cinnamate-4-hydroxylase (C4H), and 4-coumarate CoA ligase (4CL), and the contents of flavonoids and total phenolic compounds in pear fruit. Furthermore, thifluzamide increased the expression of <i>PcPAL</i>, <i>PcC4H</i>, <i>Pc4CL</i>, <i>Pcβ-1,3-GA</i>, <i>PcLCH</i>, <i>PcF3H</i>, and <i>PcDFR</i> involved in phenylpropanoid metabolism in pear fruit. In conclusion, thifluzamide treatment reduced the infection ability of <i>R. solani</i> by inhibiting the expression of the genes encoding cell wall-degrading enzymes in <i>R. solani</i>. At the same time, it inhibited the activities of cell wall-degrading enzymes induced resistance against <i>R. solani</i> infection in ‘Huangguan’ pears by promoting phenylpropane metabolism.
ISSN:2076-0817

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