Regulation of Long Noncoding RNAs Responsive to Phytoplasma Infection in Paulownia tomentosa
Paulownia witches’ broom caused by phytoplasma infection affects the production of Paulownia trees worldwide. Emerging evidence showed that long noncoding RNAs (lncRNA) play a protagonist role in regulating the expression of genes in plants. So far, the identification of lncRNAs has been limited to...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | International Journal of Genomics |
| Online Access: | http://dx.doi.org/10.1155/2018/3174352 |
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| author | Guoqiang Fan Yabing Cao Zhe Wang |
| author_facet | Guoqiang Fan Yabing Cao Zhe Wang |
| author_sort | Guoqiang Fan |
| collection | DOAJ |
| description | Paulownia witches’ broom caused by phytoplasma infection affects the production of Paulownia trees worldwide. Emerging evidence showed that long noncoding RNAs (lncRNA) play a protagonist role in regulating the expression of genes in plants. So far, the identification of lncRNAs has been limited to a few model plant species, and their roles in mediating responses to Paulownia tomentosa that free of phytoplasma infection are yet to be characterized. Here, whole-genome identification of lncRNAs, based on strand-specific RNA sequencing, from four Paulownia tomentosa samples, was performed and identified 3689 lncRNAs. These lncRNAs showed low conservation among plant species and some of them were miRNA precursors. Further analysis revealed that the 112 identified lncRNAs were related to phytoplasma infection. We predicted the target genes of these phytoplasma-responsive lncRNAs, and our analysis showed that 51 of the predicted target genes were alternatively spliced. Moreover, we found the expression of the lncRNAs plays vital roles in regulating the genes involved in the reactive oxygen species induced hypersensitive response and effector-triggered immunity in phytoplasma-infected Paulownia. This study indicated that diverse sets of lncRNAs were responsive to Paulownia witches’ broom, and the results will provide a starting point to understand the functions and regulatory mechanisms of Paulownia lncRNAs in the future. |
| format | Article |
| id | doaj-art-8c540c3138bc45f8ae92eec04cf2d6d0 |
| institution | Kabale University |
| issn | 2314-436X 2314-4378 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Genomics |
| spelling | doaj-art-8c540c3138bc45f8ae92eec04cf2d6d02025-02-03T01:13:12ZengWileyInternational Journal of Genomics2314-436X2314-43782018-01-01201810.1155/2018/31743523174352Regulation of Long Noncoding RNAs Responsive to Phytoplasma Infection in Paulownia tomentosaGuoqiang Fan0Yabing Cao1Zhe Wang2Institute of Paulownia, Henan Agricultural University, Zhengzhou, Henan 450002, ChinaInstitute of Paulownia, Henan Agricultural University, Zhengzhou, Henan 450002, ChinaInstitute of Paulownia, Henan Agricultural University, Zhengzhou, Henan 450002, ChinaPaulownia witches’ broom caused by phytoplasma infection affects the production of Paulownia trees worldwide. Emerging evidence showed that long noncoding RNAs (lncRNA) play a protagonist role in regulating the expression of genes in plants. So far, the identification of lncRNAs has been limited to a few model plant species, and their roles in mediating responses to Paulownia tomentosa that free of phytoplasma infection are yet to be characterized. Here, whole-genome identification of lncRNAs, based on strand-specific RNA sequencing, from four Paulownia tomentosa samples, was performed and identified 3689 lncRNAs. These lncRNAs showed low conservation among plant species and some of them were miRNA precursors. Further analysis revealed that the 112 identified lncRNAs were related to phytoplasma infection. We predicted the target genes of these phytoplasma-responsive lncRNAs, and our analysis showed that 51 of the predicted target genes were alternatively spliced. Moreover, we found the expression of the lncRNAs plays vital roles in regulating the genes involved in the reactive oxygen species induced hypersensitive response and effector-triggered immunity in phytoplasma-infected Paulownia. This study indicated that diverse sets of lncRNAs were responsive to Paulownia witches’ broom, and the results will provide a starting point to understand the functions and regulatory mechanisms of Paulownia lncRNAs in the future.http://dx.doi.org/10.1155/2018/3174352 |
| spellingShingle | Guoqiang Fan Yabing Cao Zhe Wang Regulation of Long Noncoding RNAs Responsive to Phytoplasma Infection in Paulownia tomentosa International Journal of Genomics |
| title | Regulation of Long Noncoding RNAs Responsive to Phytoplasma Infection in Paulownia tomentosa |
| title_full | Regulation of Long Noncoding RNAs Responsive to Phytoplasma Infection in Paulownia tomentosa |
| title_fullStr | Regulation of Long Noncoding RNAs Responsive to Phytoplasma Infection in Paulownia tomentosa |
| title_full_unstemmed | Regulation of Long Noncoding RNAs Responsive to Phytoplasma Infection in Paulownia tomentosa |
| title_short | Regulation of Long Noncoding RNAs Responsive to Phytoplasma Infection in Paulownia tomentosa |
| title_sort | regulation of long noncoding rnas responsive to phytoplasma infection in paulownia tomentosa |
| url | http://dx.doi.org/10.1155/2018/3174352 |
| work_keys_str_mv | AT guoqiangfan regulationoflongnoncodingrnasresponsivetophytoplasmainfectioninpaulowniatomentosa AT yabingcao regulationoflongnoncodingrnasresponsivetophytoplasmainfectioninpaulowniatomentosa AT zhewang regulationoflongnoncodingrnasresponsivetophytoplasmainfectioninpaulowniatomentosa |