Analysis of Differences in Volatile Components of Five Lauraceae Plants From Different Genera Based on HS-SPME-GC-MS

Lauraceae plants are diverse in species and rich in volatile components, which possess functions such as insect repellency, antioxidant activity, and antibacterial properties. However, currently, the methods for analyzing the volatile components of Lauraceae plants are relatively single. The essenti...

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Main Authors: Zhengjiu Wang, Hao Liu, Anping Liu, Peng Liu, Jiuyang Zhao, Shaoli Fan, Jinhui Wang, Bahetiyar Keremu, Lu Yang, Le Li
Format: Article
Language:English
Published: Wiley 2025-01-01
Series:Journal of Analytical Methods in Chemistry
Online Access:http://dx.doi.org/10.1155/jamc/9045731
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author Zhengjiu Wang
Hao Liu
Anping Liu
Peng Liu
Jiuyang Zhao
Shaoli Fan
Jinhui Wang
Bahetiyar Keremu
Lu Yang
Le Li
author_facet Zhengjiu Wang
Hao Liu
Anping Liu
Peng Liu
Jiuyang Zhao
Shaoli Fan
Jinhui Wang
Bahetiyar Keremu
Lu Yang
Le Li
author_sort Zhengjiu Wang
collection DOAJ
description Lauraceae plants are diverse in species and rich in volatile components, which possess functions such as insect repellency, antioxidant activity, and antibacterial properties. However, currently, the methods for analyzing the volatile components of Lauraceae plants are relatively single. The essential oils are mainly extracted by steam distillation, but the pretreatment is relatively complex and cumbersome. Therefore, it is essential to find a simple and cost-effective method. By comparing different extraction methods, HS-SPME-GC-MS was selected as the optimal extraction condition. Regarding Head-space Solid-Phase Microextraction and Gas Chromatography-Mass Spectrometry (HS-SPME-GC-MS), single-factor condition optimization and response surface analysis were carried out for different fiber coatings, equilibrium time, extraction temperature, and extraction time. Eventually, 75-μm CAR/PDMS fiber head was chosen, with an equilibrium time of 15 min, and extraction was conducted at 70°C for 57 min as the optimal HS-SPME extraction conditions. Furthermore, a differential analysis of the volatile components of five Lauraceae plants from different genera was performed, and differential metabolites were screened out respectively. This can effectively separate Cinnamomum and Litsea from the other three genera, providing certain assistance for the chemotaxonomy of the volatile components of Lauraceae plants and the subsequent development of medicinal plant resources.
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institution Kabale University
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publishDate 2025-01-01
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series Journal of Analytical Methods in Chemistry
spelling doaj-art-8c4a189127d64c00b6e6ce2ef7090ef22025-08-20T04:02:41ZengWileyJournal of Analytical Methods in Chemistry2090-88732025-01-01202510.1155/jamc/9045731Analysis of Differences in Volatile Components of Five Lauraceae Plants From Different Genera Based on HS-SPME-GC-MSZhengjiu Wang0Hao Liu1Anping Liu2Peng Liu3Jiuyang Zhao4Shaoli Fan5Jinhui Wang6Bahetiyar Keremu7Lu Yang8Le Li9Key Laboratory of Xinjiang Phytomedicine Resource and UtilizationXinjiang Association for Science and TechnologyKey Laboratory of Forest Resources and Utilization in Xinjiang of National Forestry and Grassland AdministrationKey Laboratory of Forest Resources and Utilization in Xinjiang of National Forestry and Grassland AdministrationKey Laboratory of Forest Resources and Utilization in Xinjiang of National Forestry and Grassland AdministrationKey Laboratory of Forest Resources and Utilization in Xinjiang of National Forestry and Grassland AdministrationKey Laboratory of Xinjiang Phytomedicine Resource and UtilizationKey Laboratory of Forest Resources and Utilization in Xinjiang of National Forestry and Grassland AdministrationKey Laboratory of Forest Resources and Utilization in Xinjiang of National Forestry and Grassland AdministrationKey Laboratory of Xinjiang Phytomedicine Resource and UtilizationLauraceae plants are diverse in species and rich in volatile components, which possess functions such as insect repellency, antioxidant activity, and antibacterial properties. However, currently, the methods for analyzing the volatile components of Lauraceae plants are relatively single. The essential oils are mainly extracted by steam distillation, but the pretreatment is relatively complex and cumbersome. Therefore, it is essential to find a simple and cost-effective method. By comparing different extraction methods, HS-SPME-GC-MS was selected as the optimal extraction condition. Regarding Head-space Solid-Phase Microextraction and Gas Chromatography-Mass Spectrometry (HS-SPME-GC-MS), single-factor condition optimization and response surface analysis were carried out for different fiber coatings, equilibrium time, extraction temperature, and extraction time. Eventually, 75-μm CAR/PDMS fiber head was chosen, with an equilibrium time of 15 min, and extraction was conducted at 70°C for 57 min as the optimal HS-SPME extraction conditions. Furthermore, a differential analysis of the volatile components of five Lauraceae plants from different genera was performed, and differential metabolites were screened out respectively. This can effectively separate Cinnamomum and Litsea from the other three genera, providing certain assistance for the chemotaxonomy of the volatile components of Lauraceae plants and the subsequent development of medicinal plant resources.http://dx.doi.org/10.1155/jamc/9045731
spellingShingle Zhengjiu Wang
Hao Liu
Anping Liu
Peng Liu
Jiuyang Zhao
Shaoli Fan
Jinhui Wang
Bahetiyar Keremu
Lu Yang
Le Li
Analysis of Differences in Volatile Components of Five Lauraceae Plants From Different Genera Based on HS-SPME-GC-MS
Journal of Analytical Methods in Chemistry
title Analysis of Differences in Volatile Components of Five Lauraceae Plants From Different Genera Based on HS-SPME-GC-MS
title_full Analysis of Differences in Volatile Components of Five Lauraceae Plants From Different Genera Based on HS-SPME-GC-MS
title_fullStr Analysis of Differences in Volatile Components of Five Lauraceae Plants From Different Genera Based on HS-SPME-GC-MS
title_full_unstemmed Analysis of Differences in Volatile Components of Five Lauraceae Plants From Different Genera Based on HS-SPME-GC-MS
title_short Analysis of Differences in Volatile Components of Five Lauraceae Plants From Different Genera Based on HS-SPME-GC-MS
title_sort analysis of differences in volatile components of five lauraceae plants from different genera based on hs spme gc ms
url http://dx.doi.org/10.1155/jamc/9045731
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