Expression of miRNA and TSP-1 mRNA in renal tubular epithelial cells
Objective To study the expression of miR-18 a,miR-19 a,miR-194 and TSP-1 in renal tubular epithelial cells treated with high sugar and TGF-β1.Methods The mRNA expression levels of TSP-1,miR-18 a,miR-19 aand miR-194 in human renal tubular epithelial cells in low glucose group(final concentration of g...
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Editorial Department of Journal of Clinical Nephrology
2018-01-01
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| Series: | Linchuang shenzangbing zazhi |
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| author | ZENG Rong WANG Gou-qin LIANG Yao-jun KONG Yu-ke DUAN Lei |
| author_facet | ZENG Rong WANG Gou-qin LIANG Yao-jun KONG Yu-ke DUAN Lei |
| author_sort | ZENG Rong |
| collection | DOAJ |
| description | Objective To study the expression of miR-18 a,miR-19 a,miR-194 and TSP-1 in renal tubular epithelial cells treated with high sugar and TGF-β1.Methods The mRNA expression levels of TSP-1,miR-18 a,miR-19 aand miR-194 in human renal tubular epithelial cells in low glucose group(final concentration of glucose:5 mmol/L),high glucose group(final concentration of glucose:30 mmol/L)and high glucose+TGF-β1 group were detected by RT-PCR.Results With the rise of blood glucose and prolongation of the incubation time,the expression level of TSP1 mRNA increased gradually,and miR-194 decreased gradually,but the expression levels of miR-18 aand miR-19 ahad no significant change.The mRNA expression of TSP1 was significantly up-regulated,and that of miR-194 was significantly down-regulated in high glucose+TGF-β1 group as compared with high glucose group.Conclusions TGF-β1 positively regulates the expression of TSP1,and negatively regulates miR-194.We need to confirm whether TSP1 is a target gene of miR-194 in the future. |
| format | Article |
| id | doaj-art-8c390f5f95cd4d139ea26638294f2c53 |
| institution | OA Journals |
| issn | 1671-2390 |
| language | zho |
| publishDate | 2018-01-01 |
| publisher | Editorial Department of Journal of Clinical Nephrology |
| record_format | Article |
| series | Linchuang shenzangbing zazhi |
| spelling | doaj-art-8c390f5f95cd4d139ea26638294f2c532025-08-20T02:28:51ZzhoEditorial Department of Journal of Clinical NephrologyLinchuang shenzangbing zazhi1671-23902018-01-0111111457916468Expression of miRNA and TSP-1 mRNA in renal tubular epithelial cellsZENG RongWANG Gou-qinLIANG Yao-junKONG Yu-keDUAN LeiObjective To study the expression of miR-18 a,miR-19 a,miR-194 and TSP-1 in renal tubular epithelial cells treated with high sugar and TGF-β1.Methods The mRNA expression levels of TSP-1,miR-18 a,miR-19 aand miR-194 in human renal tubular epithelial cells in low glucose group(final concentration of glucose:5 mmol/L),high glucose group(final concentration of glucose:30 mmol/L)and high glucose+TGF-β1 group were detected by RT-PCR.Results With the rise of blood glucose and prolongation of the incubation time,the expression level of TSP1 mRNA increased gradually,and miR-194 decreased gradually,but the expression levels of miR-18 aand miR-19 ahad no significant change.The mRNA expression of TSP1 was significantly up-regulated,and that of miR-194 was significantly down-regulated in high glucose+TGF-β1 group as compared with high glucose group.Conclusions TGF-β1 positively regulates the expression of TSP1,and negatively regulates miR-194.We need to confirm whether TSP1 is a target gene of miR-194 in the future.http://www.lcszb.com/thesisDetails?columnId=57916468&Fpath=home&index=0Diabetic nephropathytransforming growth factor-β1thrombospondin1 |
| spellingShingle | ZENG Rong WANG Gou-qin LIANG Yao-jun KONG Yu-ke DUAN Lei Expression of miRNA and TSP-1 mRNA in renal tubular epithelial cells Linchuang shenzangbing zazhi Diabetic nephropathy transforming growth factor-β1 thrombospondin1 |
| title | Expression of miRNA and TSP-1 mRNA in renal tubular epithelial cells |
| title_full | Expression of miRNA and TSP-1 mRNA in renal tubular epithelial cells |
| title_fullStr | Expression of miRNA and TSP-1 mRNA in renal tubular epithelial cells |
| title_full_unstemmed | Expression of miRNA and TSP-1 mRNA in renal tubular epithelial cells |
| title_short | Expression of miRNA and TSP-1 mRNA in renal tubular epithelial cells |
| title_sort | expression of mirna and tsp 1 mrna in renal tubular epithelial cells |
| topic | Diabetic nephropathy transforming growth factor-β1 thrombospondin1 |
| url | http://www.lcszb.com/thesisDetails?columnId=57916468&Fpath=home&index=0 |
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