Pond Water eDNA Reflects Broad Consistency with Surrounding Terrestrial Plant Ecosystems
This study evaluates the potential of using pond water eDNA to reflect the surrounding terrestrial plant communities, aiming to develop a sustainable, large-scale, and long-term monitoring method for plant diversity in forest ecosystems. Water samples were collected four times from two ponds with di...
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| Format: | Article |
| Language: | English |
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MDPI AG
2025-01-01
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| Series: | Biology |
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| Online Access: | https://www.mdpi.com/2079-7737/14/1/62 |
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| author | Duygu Bozdogan Shogo Takizawa Norihiro Furukori Kosuke Homma Harue Abe Hitoshi Sakio Naoki Harada Kazuki Suzuki |
| author_facet | Duygu Bozdogan Shogo Takizawa Norihiro Furukori Kosuke Homma Harue Abe Hitoshi Sakio Naoki Harada Kazuki Suzuki |
| author_sort | Duygu Bozdogan |
| collection | DOAJ |
| description | This study evaluates the potential of using pond water eDNA to reflect the surrounding terrestrial plant communities, aiming to develop a sustainable, large-scale, and long-term monitoring method for plant diversity in forest ecosystems. Water samples were collected four times from two ponds with different vegetation types during the late spring to autumn seasons in Japan. eDNA was extracted from dissolved particles fractionated by sequential filtration through pore sizes of 200 µm, 5 µm, and 0.45 µm, followed by high-throughput amplicon sequencing targeting the plant rbcL gene. By comparing field surveys with the eDNA data, we identified 79% and 63% of plant families and genera, respectively, suggesting that pond water eDNA may reflect the surrounding terrestrial plant ecosystem. Additionally, different trends were observed in the seasonal variation of plant taxa and their composition detected in eDNA, based on particle size. This study highlights the potential of pond water eDNA to provide valuable insights into forest plant richness and seasonal dynamics, offering a novel approach for ecological monitoring. |
| format | Article |
| id | doaj-art-8c2e94805de74cbc9e8f4db752e041d9 |
| institution | Kabale University |
| issn | 2079-7737 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj-art-8c2e94805de74cbc9e8f4db752e041d92025-01-24T13:23:29ZengMDPI AGBiology2079-77372025-01-011416210.3390/biology14010062Pond Water eDNA Reflects Broad Consistency with Surrounding Terrestrial Plant EcosystemsDuygu Bozdogan0Shogo Takizawa1Norihiro Furukori2Kosuke Homma3Harue Abe4Hitoshi Sakio5Naoki Harada6Kazuki Suzuki7Graduate School of Science and Technology, Niigata University, Niigata 950-2181, JapanFaculty of Agriculture, Niigata University, Niigata 950-2181, JapanSado Island Center for Ecological Sustainability, Niigata University, Niigata 952-0103, JapanSado Island Center for Ecological Sustainability, Niigata University, Niigata 952-0103, JapanSado Island Center for Ecological Sustainability, Niigata University, Niigata 952-0103, JapanSado Island Center for Ecological Sustainability, Niigata University, Niigata 952-0103, JapanInstitute of Science and Technology, Niigata University, Niigata 950-2181, JapanInstitute of Science and Technology, Niigata University, Niigata 950-2181, JapanThis study evaluates the potential of using pond water eDNA to reflect the surrounding terrestrial plant communities, aiming to develop a sustainable, large-scale, and long-term monitoring method for plant diversity in forest ecosystems. Water samples were collected four times from two ponds with different vegetation types during the late spring to autumn seasons in Japan. eDNA was extracted from dissolved particles fractionated by sequential filtration through pore sizes of 200 µm, 5 µm, and 0.45 µm, followed by high-throughput amplicon sequencing targeting the plant rbcL gene. By comparing field surveys with the eDNA data, we identified 79% and 63% of plant families and genera, respectively, suggesting that pond water eDNA may reflect the surrounding terrestrial plant ecosystem. Additionally, different trends were observed in the seasonal variation of plant taxa and their composition detected in eDNA, based on particle size. This study highlights the potential of pond water eDNA to provide valuable insights into forest plant richness and seasonal dynamics, offering a novel approach for ecological monitoring.https://www.mdpi.com/2079-7737/14/1/62ecological monitoringeDNAplant communityfresh waterfiltration |
| spellingShingle | Duygu Bozdogan Shogo Takizawa Norihiro Furukori Kosuke Homma Harue Abe Hitoshi Sakio Naoki Harada Kazuki Suzuki Pond Water eDNA Reflects Broad Consistency with Surrounding Terrestrial Plant Ecosystems Biology ecological monitoring eDNA plant community fresh water filtration |
| title | Pond Water eDNA Reflects Broad Consistency with Surrounding Terrestrial Plant Ecosystems |
| title_full | Pond Water eDNA Reflects Broad Consistency with Surrounding Terrestrial Plant Ecosystems |
| title_fullStr | Pond Water eDNA Reflects Broad Consistency with Surrounding Terrestrial Plant Ecosystems |
| title_full_unstemmed | Pond Water eDNA Reflects Broad Consistency with Surrounding Terrestrial Plant Ecosystems |
| title_short | Pond Water eDNA Reflects Broad Consistency with Surrounding Terrestrial Plant Ecosystems |
| title_sort | pond water edna reflects broad consistency with surrounding terrestrial plant ecosystems |
| topic | ecological monitoring eDNA plant community fresh water filtration |
| url | https://www.mdpi.com/2079-7737/14/1/62 |
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