The WRKY28-BRC1 Transcription Factor Module Controls Shoot Branching in <i>Brassica napus</i>
The trade-off between growth and defense is common in plants. We previously demonstrated that BnaA03.WRKY28 weakened resistance strength but promoted shoot branching in <i>Brassica napus</i> (rapeseed). However, the molecular mechanism by which WRKY28 promotes branching formation is stil...
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2025-02-01
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| author | Ka Zhang Jinfang Zhang Cheng Cui Liang Chai Benchuan Zheng Liangcai Jiang Haojie Li |
| author_facet | Ka Zhang Jinfang Zhang Cheng Cui Liang Chai Benchuan Zheng Liangcai Jiang Haojie Li |
| author_sort | Ka Zhang |
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| description | The trade-off between growth and defense is common in plants. We previously demonstrated that BnaA03.WRKY28 weakened resistance strength but promoted shoot branching in <i>Brassica napus</i> (rapeseed). However, the molecular mechanism by which WRKY28 promotes branching formation is still obscure. In this study, we found that <i>BnaA01.BRC1</i>, <i>BnaC01.BRC1, and BnaC03.BRC1</i> are mainly expressed in the leaf axils and contained W-box <i>cis</i>-acting elements in the promoter regions. BnaA03.WRKY28 directly bound to the promoter regions of these three copies and inhibited their expression. The <i>brc1</i> mutants, the <i>BnaA01.BRC1</i>, <i>BnaC01.BRC1 BnaA03.BRC1</i> and <i>BnaC03.BRC1</i> were simultaneously knocked out, mediated by CRISPR/Cas9, and exhibited excessive branching. The expression level of the ABA biosynthesis encoding gene <i>NCED3</i> was significantly reduced in the mutant compared to that in the WT. Instead, the expression level of the ABA catabolism encoding gene <i>CYP707A3</i> was significantly higher than that in WT. These results suggest that the excessive branching of the <i>brc1</i> mutant may be caused by the release of ABA-mediated bud dormancy. This study provides direct evidence for the potential mechanism of the WRKY28-BRC1 transcription factor module contributing to shoot branching in rapeseed. |
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| spelling | doaj-art-8c2dbd0d7f3247b18ec15d07ae6f936b2025-08-20T02:12:33ZengMDPI AGPlants2223-77472025-02-0114348610.3390/plants14030486The WRKY28-BRC1 Transcription Factor Module Controls Shoot Branching in <i>Brassica napus</i>Ka Zhang0Jinfang Zhang1Cheng Cui2Liang Chai3Benchuan Zheng4Liangcai Jiang5Haojie Li6Crop Research Institute, Sichuan Academy of Agricultural Sciences, Environment-Friendly Crop Germplasm Innovation and Genetic Improvement Key Laboratory of Sichuan Province, Chengdu 610066, ChinaCrop Research Institute, Sichuan Academy of Agricultural Sciences, Environment-Friendly Crop Germplasm Innovation and Genetic Improvement Key Laboratory of Sichuan Province, Chengdu 610066, ChinaCrop Research Institute, Sichuan Academy of Agricultural Sciences, Environment-Friendly Crop Germplasm Innovation and Genetic Improvement Key Laboratory of Sichuan Province, Chengdu 610066, ChinaCrop Research Institute, Sichuan Academy of Agricultural Sciences, Environment-Friendly Crop Germplasm Innovation and Genetic Improvement Key Laboratory of Sichuan Province, Chengdu 610066, ChinaCrop Research Institute, Sichuan Academy of Agricultural Sciences, Environment-Friendly Crop Germplasm Innovation and Genetic Improvement Key Laboratory of Sichuan Province, Chengdu 610066, ChinaCrop Research Institute, Sichuan Academy of Agricultural Sciences, Environment-Friendly Crop Germplasm Innovation and Genetic Improvement Key Laboratory of Sichuan Province, Chengdu 610066, ChinaCrop Research Institute, Sichuan Academy of Agricultural Sciences, Environment-Friendly Crop Germplasm Innovation and Genetic Improvement Key Laboratory of Sichuan Province, Chengdu 610066, ChinaThe trade-off between growth and defense is common in plants. We previously demonstrated that BnaA03.WRKY28 weakened resistance strength but promoted shoot branching in <i>Brassica napus</i> (rapeseed). However, the molecular mechanism by which WRKY28 promotes branching formation is still obscure. In this study, we found that <i>BnaA01.BRC1</i>, <i>BnaC01.BRC1, and BnaC03.BRC1</i> are mainly expressed in the leaf axils and contained W-box <i>cis</i>-acting elements in the promoter regions. BnaA03.WRKY28 directly bound to the promoter regions of these three copies and inhibited their expression. The <i>brc1</i> mutants, the <i>BnaA01.BRC1</i>, <i>BnaC01.BRC1 BnaA03.BRC1</i> and <i>BnaC03.BRC1</i> were simultaneously knocked out, mediated by CRISPR/Cas9, and exhibited excessive branching. The expression level of the ABA biosynthesis encoding gene <i>NCED3</i> was significantly reduced in the mutant compared to that in the WT. Instead, the expression level of the ABA catabolism encoding gene <i>CYP707A3</i> was significantly higher than that in WT. These results suggest that the excessive branching of the <i>brc1</i> mutant may be caused by the release of ABA-mediated bud dormancy. This study provides direct evidence for the potential mechanism of the WRKY28-BRC1 transcription factor module contributing to shoot branching in rapeseed.https://www.mdpi.com/2223-7747/14/3/486<i>Brassica napus</i>axillary meristembranchingWRKY transcription factorTCP transcription factor |
| spellingShingle | Ka Zhang Jinfang Zhang Cheng Cui Liang Chai Benchuan Zheng Liangcai Jiang Haojie Li The WRKY28-BRC1 Transcription Factor Module Controls Shoot Branching in <i>Brassica napus</i> Plants <i>Brassica napus</i> axillary meristem branching WRKY transcription factor TCP transcription factor |
| title | The WRKY28-BRC1 Transcription Factor Module Controls Shoot Branching in <i>Brassica napus</i> |
| title_full | The WRKY28-BRC1 Transcription Factor Module Controls Shoot Branching in <i>Brassica napus</i> |
| title_fullStr | The WRKY28-BRC1 Transcription Factor Module Controls Shoot Branching in <i>Brassica napus</i> |
| title_full_unstemmed | The WRKY28-BRC1 Transcription Factor Module Controls Shoot Branching in <i>Brassica napus</i> |
| title_short | The WRKY28-BRC1 Transcription Factor Module Controls Shoot Branching in <i>Brassica napus</i> |
| title_sort | wrky28 brc1 transcription factor module controls shoot branching in i brassica napus i |
| topic | <i>Brassica napus</i> axillary meristem branching WRKY transcription factor TCP transcription factor |
| url | https://www.mdpi.com/2223-7747/14/3/486 |
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