Antibodies with specificity to glycan motifs that decorate OMV cargo proteins
ABSTRACT Porphyromonas gingivalis is a major etiological agent of periodontal disease, and infections with this bacterium are associated with systemic pathologies, including atherosclerosis, rheumatoid arthritis, and Alzheimer’s disease. P. gingivalis has a variety of immune evasion mechanisms and e...
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American Society for Microbiology
2025-03-01
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| Series: | mSphere |
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| Online Access: | https://journals.asm.org/doi/10.1128/msphere.00907-24 |
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| author | Hyun Young Kim Christina M. Rothenberger Mary E. Davey Manda Yu |
| author_facet | Hyun Young Kim Christina M. Rothenberger Mary E. Davey Manda Yu |
| author_sort | Hyun Young Kim |
| collection | DOAJ |
| description | ABSTRACT Porphyromonas gingivalis is a major etiological agent of periodontal disease, and infections with this bacterium are associated with systemic pathologies, including atherosclerosis, rheumatoid arthritis, and Alzheimer’s disease. P. gingivalis has a variety of immune evasion mechanisms and exhibits highly variable cell surface characteristics that are strain dependent, complicating the development of effective vaccines and therapeutics. Here, we show that a subset of immunoglobulin M (IgM) antibodies in antiserum raised against P. gingivalis strain W83 selectively recognize the outer membrane vesicles (OMVs). Pre-adsorption with a mutant strain lacking an OMV-specific lipoprotein (PG1881) that has been shown to be glycosylated significantly enhanced IgM specificity toward PG1881 and the OMVs. In addition, the IgM reactivity against the OMVs derived from a mutant lacking enzymes required for O-glycosylation was markedly reduced, indicating that the IgM targets the glycan motifs on proteins carried on OMVs. Importantly, the IgM exhibited specific recognition of OMVs from both P. gingivalis and Porphyromonas endodontalis, while showing low reactivity toward other genera belonging to the phylum Bacteroidetes. This study revealed a potential host evasion strategy and highlights the potential for utilizing O-glycans in vaccine development and OMV-targeted antibodies in therapeutic interventions to combat P. gingivalis infections.IMPORTANCEO-glycosylation of cell surface proteins by bacteria is known to play a role in various functions including colonization and immune evasion. This study highlights the identification of IgM antibodies that specifically recognize O-glycosylated proteins that are selectively carried on outer membrane vesicles (OMVs). The findings suggest a potential host evasion mechanism and open new avenues for using OMVs in vaccine development and targeting O-glycans with antibodies as a therapeutic strategy against the subgingival pathobiont P. gingivalis. |
| format | Article |
| id | doaj-art-8c2a2fa2e9e54350be66015cf5577a0f |
| institution | Kabale University |
| issn | 2379-5042 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | mSphere |
| spelling | doaj-art-8c2a2fa2e9e54350be66015cf5577a0f2025-08-20T03:40:25ZengAmerican Society for MicrobiologymSphere2379-50422025-03-0110310.1128/msphere.00907-24Antibodies with specificity to glycan motifs that decorate OMV cargo proteinsHyun Young Kim0Christina M. Rothenberger1Mary E. Davey2Manda Yu3Department of Microbiology, ADA Forsyth Institute, Cambridge, Massachusetts, USADepartment of Microbiology, ADA Forsyth Institute, Cambridge, Massachusetts, USADepartment of Microbiology, ADA Forsyth Institute, Cambridge, Massachusetts, USADepartment of Microbiology, ADA Forsyth Institute, Cambridge, Massachusetts, USAABSTRACT Porphyromonas gingivalis is a major etiological agent of periodontal disease, and infections with this bacterium are associated with systemic pathologies, including atherosclerosis, rheumatoid arthritis, and Alzheimer’s disease. P. gingivalis has a variety of immune evasion mechanisms and exhibits highly variable cell surface characteristics that are strain dependent, complicating the development of effective vaccines and therapeutics. Here, we show that a subset of immunoglobulin M (IgM) antibodies in antiserum raised against P. gingivalis strain W83 selectively recognize the outer membrane vesicles (OMVs). Pre-adsorption with a mutant strain lacking an OMV-specific lipoprotein (PG1881) that has been shown to be glycosylated significantly enhanced IgM specificity toward PG1881 and the OMVs. In addition, the IgM reactivity against the OMVs derived from a mutant lacking enzymes required for O-glycosylation was markedly reduced, indicating that the IgM targets the glycan motifs on proteins carried on OMVs. Importantly, the IgM exhibited specific recognition of OMVs from both P. gingivalis and Porphyromonas endodontalis, while showing low reactivity toward other genera belonging to the phylum Bacteroidetes. This study revealed a potential host evasion strategy and highlights the potential for utilizing O-glycans in vaccine development and OMV-targeted antibodies in therapeutic interventions to combat P. gingivalis infections.IMPORTANCEO-glycosylation of cell surface proteins by bacteria is known to play a role in various functions including colonization and immune evasion. This study highlights the identification of IgM antibodies that specifically recognize O-glycosylated proteins that are selectively carried on outer membrane vesicles (OMVs). The findings suggest a potential host evasion mechanism and open new avenues for using OMVs in vaccine development and targeting O-glycans with antibodies as a therapeutic strategy against the subgingival pathobiont P. gingivalis.https://journals.asm.org/doi/10.1128/msphere.00907-24P. gingivalisOMVIgM antibodiesO-glycosylationPG1881 |
| spellingShingle | Hyun Young Kim Christina M. Rothenberger Mary E. Davey Manda Yu Antibodies with specificity to glycan motifs that decorate OMV cargo proteins mSphere P. gingivalis OMV IgM antibodies O-glycosylation PG1881 |
| title | Antibodies with specificity to glycan motifs that decorate OMV cargo proteins |
| title_full | Antibodies with specificity to glycan motifs that decorate OMV cargo proteins |
| title_fullStr | Antibodies with specificity to glycan motifs that decorate OMV cargo proteins |
| title_full_unstemmed | Antibodies with specificity to glycan motifs that decorate OMV cargo proteins |
| title_short | Antibodies with specificity to glycan motifs that decorate OMV cargo proteins |
| title_sort | antibodies with specificity to glycan motifs that decorate omv cargo proteins |
| topic | P. gingivalis OMV IgM antibodies O-glycosylation PG1881 |
| url | https://journals.asm.org/doi/10.1128/msphere.00907-24 |
| work_keys_str_mv | AT hyunyoungkim antibodieswithspecificitytoglycanmotifsthatdecorateomvcargoproteins AT christinamrothenberger antibodieswithspecificitytoglycanmotifsthatdecorateomvcargoproteins AT maryedavey antibodieswithspecificitytoglycanmotifsthatdecorateomvcargoproteins AT mandayu antibodieswithspecificitytoglycanmotifsthatdecorateomvcargoproteins |