Simultaneous Determination of 32 Polyphenolic Compounds in Berries via HPLC–MS/MS
An HPLC-MS/MS method for the simultaneous determination of 32 polyphenolic compounds in berries was established. For method validation, the berry samples were extracted with 80% ethanol, purified on an HLB column, and separated on a C18 column via gradient elution with an acetonitrile–water mobile p...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-04-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/30/9/2008 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850136271236628480 |
|---|---|
| author | Yuan Wang Lijie Xing Jinlei Zhang Yongfa Chen Shiling Lu |
| author_facet | Yuan Wang Lijie Xing Jinlei Zhang Yongfa Chen Shiling Lu |
| author_sort | Yuan Wang |
| collection | DOAJ |
| description | An HPLC-MS/MS method for the simultaneous determination of 32 polyphenolic compounds in berries was established. For method validation, the berry samples were extracted with 80% ethanol, purified on an HLB column, and separated on a C18 column via gradient elution with an acetonitrile–water mobile phase system before mass spectrometry detection with electrospray ionization in negative mode and multiple reaction monitoring. The results revealed that the 32 polyphenolic compounds had a good linear relationship in the concentration range of 1–500 μg/L, with R<sup>2</sup> > 0.99, limits of detection, limits of quantitation, and recoveries of 0.2–0.6 μg/kg, 0.3–1.0 μg/kg, and 82.8–104.8%, respectively, and RSDs < 5.8%. The contents of polyphenolic compounds in the berries were determined, with 23 polyphenolic compounds in sea buckthorn, 18 in mulberry, 17 in black wolfberry, and 12 in red wolfberry. Eight polyphenolic compounds were found in all 4 kinds of berries, including 4-hydroxybenzoic acid, p-coumaric acid, ferulic acid, erucic acid, rutin, hypericin, kaempferol-3-O-rutinoside, and daffinoside. Additionally, six polyphenolic compounds, catechin, syringic acid, isorhamnetin-3-O-galactoside, isorhamnetin-3-O-glucoside, cinnamic acid, and isorhamnetin, were detected only in sea buckthorn. |
| format | Article |
| id | doaj-art-8c15da0d686e4cd4b6855249fbc03fa1 |
| institution | OA Journals |
| issn | 1420-3049 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj-art-8c15da0d686e4cd4b6855249fbc03fa12025-08-20T02:31:11ZengMDPI AGMolecules1420-30492025-04-01309200810.3390/molecules30092008Simultaneous Determination of 32 Polyphenolic Compounds in Berries via HPLC–MS/MSYuan Wang0Lijie Xing1Jinlei Zhang2Yongfa Chen3Shiling Lu4College of Food Science, Shihezi University, Shihezi 832000, ChinaAnalysis and Testing Center, Xinjiang Academy of Agriculture and Reclamation Science, Shihezi 832000, ChinaAnalysis and Testing Center, Xinjiang Academy of Agriculture and Reclamation Science, Shihezi 832000, ChinaAnalysis and Testing Center, Xinjiang Academy of Agriculture and Reclamation Science, Shihezi 832000, ChinaCollege of Food Science, Shihezi University, Shihezi 832000, ChinaAn HPLC-MS/MS method for the simultaneous determination of 32 polyphenolic compounds in berries was established. For method validation, the berry samples were extracted with 80% ethanol, purified on an HLB column, and separated on a C18 column via gradient elution with an acetonitrile–water mobile phase system before mass spectrometry detection with electrospray ionization in negative mode and multiple reaction monitoring. The results revealed that the 32 polyphenolic compounds had a good linear relationship in the concentration range of 1–500 μg/L, with R<sup>2</sup> > 0.99, limits of detection, limits of quantitation, and recoveries of 0.2–0.6 μg/kg, 0.3–1.0 μg/kg, and 82.8–104.8%, respectively, and RSDs < 5.8%. The contents of polyphenolic compounds in the berries were determined, with 23 polyphenolic compounds in sea buckthorn, 18 in mulberry, 17 in black wolfberry, and 12 in red wolfberry. Eight polyphenolic compounds were found in all 4 kinds of berries, including 4-hydroxybenzoic acid, p-coumaric acid, ferulic acid, erucic acid, rutin, hypericin, kaempferol-3-O-rutinoside, and daffinoside. Additionally, six polyphenolic compounds, catechin, syringic acid, isorhamnetin-3-O-galactoside, isorhamnetin-3-O-glucoside, cinnamic acid, and isorhamnetin, were detected only in sea buckthorn.https://www.mdpi.com/1420-3049/30/9/2008HPLC-MS/MSberrypolyphenolic compoundssimultaneous determination |
| spellingShingle | Yuan Wang Lijie Xing Jinlei Zhang Yongfa Chen Shiling Lu Simultaneous Determination of 32 Polyphenolic Compounds in Berries via HPLC–MS/MS Molecules HPLC-MS/MS berry polyphenolic compounds simultaneous determination |
| title | Simultaneous Determination of 32 Polyphenolic Compounds in Berries via HPLC–MS/MS |
| title_full | Simultaneous Determination of 32 Polyphenolic Compounds in Berries via HPLC–MS/MS |
| title_fullStr | Simultaneous Determination of 32 Polyphenolic Compounds in Berries via HPLC–MS/MS |
| title_full_unstemmed | Simultaneous Determination of 32 Polyphenolic Compounds in Berries via HPLC–MS/MS |
| title_short | Simultaneous Determination of 32 Polyphenolic Compounds in Berries via HPLC–MS/MS |
| title_sort | simultaneous determination of 32 polyphenolic compounds in berries via hplc ms ms |
| topic | HPLC-MS/MS berry polyphenolic compounds simultaneous determination |
| url | https://www.mdpi.com/1420-3049/30/9/2008 |
| work_keys_str_mv | AT yuanwang simultaneousdeterminationof32polyphenoliccompoundsinberriesviahplcmsms AT lijiexing simultaneousdeterminationof32polyphenoliccompoundsinberriesviahplcmsms AT jinleizhang simultaneousdeterminationof32polyphenoliccompoundsinberriesviahplcmsms AT yongfachen simultaneousdeterminationof32polyphenoliccompoundsinberriesviahplcmsms AT shilinglu simultaneousdeterminationof32polyphenoliccompoundsinberriesviahplcmsms |