mTeSR1 is beneficial for the early development of bovine embryos and promotes the proliferation of the inner cell mass during the outgrowth of blastocysts
Background: mTeSR1 is a fully-defined, serum-free medium for the derivation and maintenance of Human embryonic stem cells (ESCs). This study investigates the impact of incorporating mTeSR1 supplement during in vitro culture (IVC) on blastocyst productivity, qualitative characteristics, and outgrowth...
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The Korean Society of Animal Reproduction and Biotechnology
2024-12-01
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| Series: | Journal of Animal Reproduction and Biotechnology |
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| Online Access: | https://www.e-jarb.org/journal/view.html?uid=2710&vmd=Full |
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| author | Dohyun Kim HakKyo Lee Dae-Jin Kwon |
| author_facet | Dohyun Kim HakKyo Lee Dae-Jin Kwon |
| author_sort | Dohyun Kim |
| collection | DOAJ |
| description | Background: mTeSR1 is a fully-defined, serum-free medium for the derivation and maintenance of Human embryonic stem cells (ESCs). This study investigates the impact of incorporating mTeSR1 supplement during in vitro culture (IVC) on blastocyst productivity, qualitative characteristics, and outgrowth potential of bovine blastocysts.
Methods: In vitro fertilized (IVF) eggs were cultured in IVC medium (control) with the addition of mTeSR1 supplement at concentrations of 1%, 2%, and 5%, respectively. The development rates of fertilized eggs and gene expression patterns of blastocysts were assessed on day 9 of culture. For outgrowth culture, blastocysts were cultured on a mouse embryonic fibroblast feeder cells (MEFs) for 7 days.
Results: In vitro development of bovine preimplantation embryos in the 2% mTeSR1 group was significantly higher than in the control (p < 0.05). The apoptotic index in the 2% mTeSR1 group was significantly lower compared to the control (p < 0.05). RTqPCR indicated that SRY-Box Transcription Factor 2 (Sox2) gene expression in the 5% mTeSR1 group was significantly higher than in the control (p < 0.05). The 5% mTeSR1 group also showed significantly higher BCL2 associated X (Bax) expression compared to the control and other mTeSR1 groups. On day 9 pi, blastocysts from the control and 2% mTeSR1 groups were cultured for 7 days. The 2% mTeSR1 group showed higher efficiency in forming dome-shaped colonies with stronger SOX2 expression compared to the control.
Conclusions: The mTeSR1 supplement supports preimplantation embryo development and prevents apoptosis in blastocysts, leading to the efficient formation of domeshaped inner cell mass (ICM) colonies. |
| format | Article |
| id | doaj-art-8c14b75a38cc4ca4a90edafc53c261ce |
| institution | Kabale University |
| issn | 2671-4639 2671-4663 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | The Korean Society of Animal Reproduction and Biotechnology |
| record_format | Article |
| series | Journal of Animal Reproduction and Biotechnology |
| spelling | doaj-art-8c14b75a38cc4ca4a90edafc53c261ce2025-01-14T15:07:30ZengThe Korean Society of Animal Reproduction and BiotechnologyJournal of Animal Reproduction and Biotechnology2671-46392671-46632024-12-0139424024710.12750/JARB.39.4.240mTeSR1 is beneficial for the early development of bovine embryos and promotes the proliferation of the inner cell mass during the outgrowth of blastocystsDohyun Kim0https://orcid.org/0000-0001-5421-9073HakKyo Lee1https://orcid.org/0000-0003-3527-1081Dae-Jin Kwon2https://orcid.org/0000-0002-9512-2283Department of Animal Biotechnology, Chonbuk National University, Jeonju 54896, KoreaDepartment of Animal Biotechnology, Chonbuk National University, Jeonju 54896, KoreaDepartment of Animal Biotechnology, Chonbuk National University, Jeonju 54896, KoreaBackground: mTeSR1 is a fully-defined, serum-free medium for the derivation and maintenance of Human embryonic stem cells (ESCs). This study investigates the impact of incorporating mTeSR1 supplement during in vitro culture (IVC) on blastocyst productivity, qualitative characteristics, and outgrowth potential of bovine blastocysts. Methods: In vitro fertilized (IVF) eggs were cultured in IVC medium (control) with the addition of mTeSR1 supplement at concentrations of 1%, 2%, and 5%, respectively. The development rates of fertilized eggs and gene expression patterns of blastocysts were assessed on day 9 of culture. For outgrowth culture, blastocysts were cultured on a mouse embryonic fibroblast feeder cells (MEFs) for 7 days. Results: In vitro development of bovine preimplantation embryos in the 2% mTeSR1 group was significantly higher than in the control (p < 0.05). The apoptotic index in the 2% mTeSR1 group was significantly lower compared to the control (p < 0.05). RTqPCR indicated that SRY-Box Transcription Factor 2 (Sox2) gene expression in the 5% mTeSR1 group was significantly higher than in the control (p < 0.05). The 5% mTeSR1 group also showed significantly higher BCL2 associated X (Bax) expression compared to the control and other mTeSR1 groups. On day 9 pi, blastocysts from the control and 2% mTeSR1 groups were cultured for 7 days. The 2% mTeSR1 group showed higher efficiency in forming dome-shaped colonies with stronger SOX2 expression compared to the control. Conclusions: The mTeSR1 supplement supports preimplantation embryo development and prevents apoptosis in blastocysts, leading to the efficient formation of domeshaped inner cell mass (ICM) colonies.https://www.e-jarb.org/journal/view.html?uid=2710&vmd=Fullcattleembryo qualityin vitro fertilized embryomtesr1outgrowth |
| spellingShingle | Dohyun Kim HakKyo Lee Dae-Jin Kwon mTeSR1 is beneficial for the early development of bovine embryos and promotes the proliferation of the inner cell mass during the outgrowth of blastocysts Journal of Animal Reproduction and Biotechnology cattle embryo quality in vitro fertilized embryo mtesr1 outgrowth |
| title | mTeSR1 is beneficial for the early development of bovine embryos and promotes the proliferation of the inner cell mass during the outgrowth of blastocysts |
| title_full | mTeSR1 is beneficial for the early development of bovine embryos and promotes the proliferation of the inner cell mass during the outgrowth of blastocysts |
| title_fullStr | mTeSR1 is beneficial for the early development of bovine embryos and promotes the proliferation of the inner cell mass during the outgrowth of blastocysts |
| title_full_unstemmed | mTeSR1 is beneficial for the early development of bovine embryos and promotes the proliferation of the inner cell mass during the outgrowth of blastocysts |
| title_short | mTeSR1 is beneficial for the early development of bovine embryos and promotes the proliferation of the inner cell mass during the outgrowth of blastocysts |
| title_sort | mtesr1 is beneficial for the early development of bovine embryos and promotes the proliferation of the inner cell mass during the outgrowth of blastocysts |
| topic | cattle embryo quality in vitro fertilized embryo mtesr1 outgrowth |
| url | https://www.e-jarb.org/journal/view.html?uid=2710&vmd=Full |
| work_keys_str_mv | AT dohyunkim mtesr1isbeneficialfortheearlydevelopmentofbovineembryosandpromotestheproliferationoftheinnercellmassduringtheoutgrowthofblastocysts AT hakkyolee mtesr1isbeneficialfortheearlydevelopmentofbovineembryosandpromotestheproliferationoftheinnercellmassduringtheoutgrowthofblastocysts AT daejinkwon mtesr1isbeneficialfortheearlydevelopmentofbovineembryosandpromotestheproliferationoftheinnercellmassduringtheoutgrowthofblastocysts |