Association of Sheep Tail Type with the T Gene Single Nucleotide Polymorphisms Loci
This study aimed to develop an effective tail typing detection technology based on the TaqMan probe technology for genotyping different sheep tail types. A total of 122 Hulun Buir short-tailed sheep and 50 Hu sheep were enrolled in the study to compare their tail morphologies, lengths, and widths. T...
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MDPI AG
2025-02-01
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| author | Daqing Wang Yifan Zhao Wenjing Fang Junxi Liang Kuo Li Caiyun Wang Guifang Cao |
| author_facet | Daqing Wang Yifan Zhao Wenjing Fang Junxi Liang Kuo Li Caiyun Wang Guifang Cao |
| author_sort | Daqing Wang |
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| description | This study aimed to develop an effective tail typing detection technology based on the TaqMan probe technology for genotyping different sheep tail types. A total of 122 Hulun Buir short-tailed sheep and 50 Hu sheep were enrolled in the study to compare their tail morphologies, lengths, and widths. Through the Sanger sequencing of loci 333 and 334 in the second exon of the T gene, distinct genotypes of various types of Hulun Buir short-tailed sheep and Hu sheep were identified. In addition, the TaqMan probe technology was employed to genotype the two SNP loci of the T gene in the two types of sheep. It was observed that the second exon of the T gene in Hulun Buir short-tailed sheep at loci 333 and 334 exhibited two genotypes, CT/CT and CT/GG, but this feature was not detected for the T gene in Hu sheep. The detection accuracy of the TaqMan probe technology for sheep tail types exceeded 70%, suggesting that it is an effective tail-typing detection technology. This study provides a solid economic foundation and theoretical ideas that will improve the breeding of short-tailed sheep. |
| format | Article |
| id | doaj-art-8bffed7137a54647b4d7cbcb4cebbeee |
| institution | OA Journals |
| issn | 2075-1729 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | MDPI AG |
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| series | Life |
| spelling | doaj-art-8bffed7137a54647b4d7cbcb4cebbeee2025-08-20T01:48:57ZengMDPI AGLife2075-17292025-02-0115334210.3390/life15030342Association of Sheep Tail Type with the T Gene Single Nucleotide Polymorphisms LociDaqing Wang0Yifan Zhao1Wenjing Fang2Junxi Liang3Kuo Li4Caiyun Wang5Guifang Cao6College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010011, ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010011, ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010011, ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010011, ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010011, ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010011, ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010011, ChinaThis study aimed to develop an effective tail typing detection technology based on the TaqMan probe technology for genotyping different sheep tail types. A total of 122 Hulun Buir short-tailed sheep and 50 Hu sheep were enrolled in the study to compare their tail morphologies, lengths, and widths. Through the Sanger sequencing of loci 333 and 334 in the second exon of the T gene, distinct genotypes of various types of Hulun Buir short-tailed sheep and Hu sheep were identified. In addition, the TaqMan probe technology was employed to genotype the two SNP loci of the T gene in the two types of sheep. It was observed that the second exon of the T gene in Hulun Buir short-tailed sheep at loci 333 and 334 exhibited two genotypes, CT/CT and CT/GG, but this feature was not detected for the T gene in Hu sheep. The detection accuracy of the TaqMan probe technology for sheep tail types exceeded 70%, suggesting that it is an effective tail-typing detection technology. This study provides a solid economic foundation and theoretical ideas that will improve the breeding of short-tailed sheep.https://www.mdpi.com/2075-1729/15/3/342T geneHulun Buir short-tailed sheepHu sheepTaqMan probe |
| spellingShingle | Daqing Wang Yifan Zhao Wenjing Fang Junxi Liang Kuo Li Caiyun Wang Guifang Cao Association of Sheep Tail Type with the T Gene Single Nucleotide Polymorphisms Loci Life T gene Hulun Buir short-tailed sheep Hu sheep TaqMan probe |
| title | Association of Sheep Tail Type with the T Gene Single Nucleotide Polymorphisms Loci |
| title_full | Association of Sheep Tail Type with the T Gene Single Nucleotide Polymorphisms Loci |
| title_fullStr | Association of Sheep Tail Type with the T Gene Single Nucleotide Polymorphisms Loci |
| title_full_unstemmed | Association of Sheep Tail Type with the T Gene Single Nucleotide Polymorphisms Loci |
| title_short | Association of Sheep Tail Type with the T Gene Single Nucleotide Polymorphisms Loci |
| title_sort | association of sheep tail type with the t gene single nucleotide polymorphisms loci |
| topic | T gene Hulun Buir short-tailed sheep Hu sheep TaqMan probe |
| url | https://www.mdpi.com/2075-1729/15/3/342 |
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