Construction of an Internal Control to Quantitate Multiple Porcine Cytokine mRNAs by RT-PCR

A multiple internal control was constructed to be used as an exogenously added control in reverse transcription polymerase chain reaction (RT-PCR) for pig cytokines. It consists of 5′ and 3′ primer sequences in the order of β2 microglobulin (β2-m), IL-1, IL-4, IL-6, IL-8, IL-2, IL-10, TNF-α, TNF-β a...

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Bibliographic Details
Main Authors: N.R. Jayagopala Reddy, Bruce N. Wilkie, Bonnie A. Mallard
Format: Article
Language:English
Published: Taylor & Francis Group 1996-11-01
Series:BioTechniques
Online Access:https://www.future-science.com/doi/10.2144/96215st05
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