Is Enoxaparin Sodium Exactly Safe For Subcutaneous Fibroblast?: A Cell Culture Study
Aim: Despite relatively low amount in the subcutaneous tissue, fibroblasts play a critical role in the continuity of intercellular connections, maintenance of tissue integrity, and forming a balanced fascial network. Enoxaparin sodium is widely used in the prophylaxis and treatment of deep vein thro...
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Alanya Alaaddin Keykubat University
2021-04-01
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| Series: | Acta Medica Alanya |
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| Online Access: | https://dergipark.org.tr/tr/download/article-file/1385077 |
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| author | Mehmet Ali Onur Hakan Işık Gökşen Ertuğrul Aylin Gürpınar Emre Kubat Duru Karasoy |
| author_facet | Mehmet Ali Onur Hakan Işık Gökşen Ertuğrul Aylin Gürpınar Emre Kubat Duru Karasoy |
| author_sort | Mehmet Ali Onur |
| collection | DOAJ |
| description | Aim: Despite relatively low amount in the subcutaneous tissue, fibroblasts play a critical role in the continuity of intercellular connections, maintenance of tissue integrity, and forming a balanced fascial network. Enoxaparin sodium is widely used in the prophylaxis and treatment of deep vein thrombosis. In the present study, we aimed to examine the cytotoxic and apoptotic effects of enoxaparin sodium on fibroblast cells in an in vitro model.Material and Methods: In a mouse model, L929 fibroblast cells were treated with enoxaparin sodium 4000 IU, 2000 IU, 1000 IU, 500 IU, and 250 IU. At 48 hours, cell morphology was evaluated; cell viability was analyzed through methylthiazole tetrazolium assay and apoptosis was assessed by propidium iodide/ acridine orange staining. Results: The test results showed that high doses (4000 IU, 2000 IU) exerted cytotoxic effects and induced apoptotic morphology. Compared to the control group, there was no significant difference in the cell viability in Dilutions III, IV, and V.Conclusion: Based on our results, despite prophylactic dose in the in vitro setting, high-dose enoxaparin showed cytotoxic and apoptotic effects. Long-term high-dose enoxaparin sodium may affect the number of subcutaneous fibroblasts, impairing the skin integrity and subcutaneous tissue healing |
| format | Article |
| id | doaj-art-8bfa6a41c40e41ea8c8952f47403b1e0 |
| institution | OA Journals |
| issn | 2587-0319 |
| language | English |
| publishDate | 2021-04-01 |
| publisher | Alanya Alaaddin Keykubat University |
| record_format | Article |
| series | Acta Medica Alanya |
| spelling | doaj-art-8bfa6a41c40e41ea8c8952f47403b1e02025-08-20T02:19:43ZengAlanya Alaaddin Keykubat UniversityActa Medica Alanya2587-03192021-04-0151182310.30565/medalanya.822679727Is Enoxaparin Sodium Exactly Safe For Subcutaneous Fibroblast?: A Cell Culture StudyMehmet Ali Onur0https://orcid.org/0000-0002-3630-7982Hakan Işık1https://orcid.org/0000-0002-7602-4434Gökşen Ertuğrul2https://orcid.org/0000-0002-5167-4780Aylin Gürpınar3https://orcid.org/0000-0001-7055-1607Emre Kubat4https://orcid.org/0000-0002-9884-8565Duru Karasoy5https://orcid.org/0000-0002-2258-4479HACETTEPE ÜNİVERSİTESİ, FEN FAKÜLTESİ, BİYOLOJİ BÖLÜMÜANKARA T.C. SB. SBÜ. GÜLHANE EĞİTİM VE ARAŞTIRMA HASTANESİKARABÜK T.C. SB. KARABÜK ÜNİVERSİTESİ EĞİTİM VE ARAŞTIRMA HASTANESİHACETTEPE ÜNİVERSİTESİ, FEN FAKÜLTESİ, BİYOLOJİ BÖLÜMÜANKARA T.C. SB. SBÜ. GÜLHANE EĞİTİM VE ARAŞTIRMA HASTANESİHACETTEPE ÜNİVERSİTESİ, FEN FAKÜLTESİ, İSTATİSTİK BÖLÜMÜAim: Despite relatively low amount in the subcutaneous tissue, fibroblasts play a critical role in the continuity of intercellular connections, maintenance of tissue integrity, and forming a balanced fascial network. Enoxaparin sodium is widely used in the prophylaxis and treatment of deep vein thrombosis. In the present study, we aimed to examine the cytotoxic and apoptotic effects of enoxaparin sodium on fibroblast cells in an in vitro model.Material and Methods: In a mouse model, L929 fibroblast cells were treated with enoxaparin sodium 4000 IU, 2000 IU, 1000 IU, 500 IU, and 250 IU. At 48 hours, cell morphology was evaluated; cell viability was analyzed through methylthiazole tetrazolium assay and apoptosis was assessed by propidium iodide/ acridine orange staining. Results: The test results showed that high doses (4000 IU, 2000 IU) exerted cytotoxic effects and induced apoptotic morphology. Compared to the control group, there was no significant difference in the cell viability in Dilutions III, IV, and V.Conclusion: Based on our results, despite prophylactic dose in the in vitro setting, high-dose enoxaparin showed cytotoxic and apoptotic effects. Long-term high-dose enoxaparin sodium may affect the number of subcutaneous fibroblasts, impairing the skin integrity and subcutaneous tissue healinghttps://dergipark.org.tr/tr/download/article-file/1385077enoxaparin sodiumcytotoxicityapoptosisskinfibroblastenoksaparin sodyumsitotoksisiteapoptozderifibroblast |
| spellingShingle | Mehmet Ali Onur Hakan Işık Gökşen Ertuğrul Aylin Gürpınar Emre Kubat Duru Karasoy Is Enoxaparin Sodium Exactly Safe For Subcutaneous Fibroblast?: A Cell Culture Study Acta Medica Alanya enoxaparin sodium cytotoxicity apoptosis skin fibroblast enoksaparin sodyum sitotoksisite apoptoz deri fibroblast |
| title | Is Enoxaparin Sodium Exactly Safe For Subcutaneous Fibroblast?: A Cell Culture Study |
| title_full | Is Enoxaparin Sodium Exactly Safe For Subcutaneous Fibroblast?: A Cell Culture Study |
| title_fullStr | Is Enoxaparin Sodium Exactly Safe For Subcutaneous Fibroblast?: A Cell Culture Study |
| title_full_unstemmed | Is Enoxaparin Sodium Exactly Safe For Subcutaneous Fibroblast?: A Cell Culture Study |
| title_short | Is Enoxaparin Sodium Exactly Safe For Subcutaneous Fibroblast?: A Cell Culture Study |
| title_sort | is enoxaparin sodium exactly safe for subcutaneous fibroblast a cell culture study |
| topic | enoxaparin sodium cytotoxicity apoptosis skin fibroblast enoksaparin sodyum sitotoksisite apoptoz deri fibroblast |
| url | https://dergipark.org.tr/tr/download/article-file/1385077 |
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