Determination of immunogenicity of an inactivated ND-vaccine developed experimentally with Newcastle disease virus (Genotype VII.2) local isolates of Bangladesh
BackgroundNewcastle disease virus (NDV) genotype VII severely affects poultry, causing respiratory and neurological symptoms with a high rate of morbidity and mortality. The research aimed to develop an inactivated ND vaccine using local isolates (Genotype VII.2) and assess its immunogenicity compar...
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Frontiers Media S.A.
2024-11-01
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2024.1482314/full |
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| author | Mohammad Aynul Haque Md. Enamul Haque Mst. Kohinoor Parvin Md. Mostofa Kamal Tanbin Rubaiya Islam Mohammad Sadekuzzaman Md. Ariful Islam Mst. Minara Khatun Muhammad Tofazzal Hossain Mohammad Asir Uddin Sham Soun Nahar A. K. M. Khasruzzaman Md. Alimul Islam |
| author_facet | Mohammad Aynul Haque Md. Enamul Haque Mst. Kohinoor Parvin Md. Mostofa Kamal Tanbin Rubaiya Islam Mohammad Sadekuzzaman Md. Ariful Islam Mst. Minara Khatun Muhammad Tofazzal Hossain Mohammad Asir Uddin Sham Soun Nahar A. K. M. Khasruzzaman Md. Alimul Islam |
| author_sort | Mohammad Aynul Haque |
| collection | DOAJ |
| description | BackgroundNewcastle disease virus (NDV) genotype VII severely affects poultry, causing respiratory and neurological symptoms with a high rate of morbidity and mortality. The research aimed to develop an inactivated ND vaccine using local isolates (Genotype VII.2) and assess its immunogenicity compared to other commercial live ND vaccines.MethodsAn inactivated vaccine using a candidate NDV (GenBank: OR924274.1) was developed according to WOAH guidelines following inactivation, sterility, purity, and safety tests. The birds were vaccinated through subcutaneous (SC) and intramuscular (IM) routes using three doses (0.25, 0.5, and 1.0 ml/bird). Immunogenicity and protective potentiality of the experimentally developed inactivated ND vaccine and live commercial ND vaccine (intra-ocularly/IO) were compared by challenge studies using three vaccination schedules: killed-followed-killed, live-followed-killed, and live-followed-live.ResultsThe birds vaccinated with 1.0 ml/bird SC showed higher antibody titers compared to those of IM-vaccinated groups. Birds vaccinated with the live-followed-killed commercial ND vaccines had slightly higher antibody titers compared to those vaccinated with killed-followed-killed and live-followed-live vaccines. Birds vaccinated with the killed-followed-killed ND vaccine showed a higher protection rate (100%) compared to live-followed-killed (83±5.77%) and live-followed-live (57±5.77%) vaccines. Birds vaccinated with killed-followed-killed group showed a slower decline rate of antibody titers than other groups. This regimen provided significantly better immunity, highlighting its potential in controlling ND outbreaks in Bangladesh's poultry.ConclusionThe study found that the inactivated ND vaccine, developed with the locally circulating isolate of genotype-VII.2 of NDV, might play an important role in effective control and management of ND in the commercial poultry population in Bangladesh. |
| format | Article |
| id | doaj-art-8bf618cc22984f9289e162f99043f40c |
| institution | OA Journals |
| issn | 1664-3224 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Immunology |
| spelling | doaj-art-8bf618cc22984f9289e162f99043f40c2025-08-20T02:12:30ZengFrontiers Media S.A.Frontiers in Immunology1664-32242024-11-011510.3389/fimmu.2024.14823141482314Determination of immunogenicity of an inactivated ND-vaccine developed experimentally with Newcastle disease virus (Genotype VII.2) local isolates of BangladeshMohammad Aynul Haque0Md. Enamul Haque1Mst. Kohinoor Parvin2Md. Mostofa Kamal3Tanbin Rubaiya Islam4Mohammad Sadekuzzaman5Md. Ariful Islam6Mst. Minara Khatun7Muhammad Tofazzal Hossain8Mohammad Asir Uddin9Sham Soun Nahar10A. K. M. Khasruzzaman11Md. Alimul Islam12Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, BangladeshDepartment of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, BangladeshDepartment of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, BangladeshDepartment of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, BangladeshDepartment of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, BangladeshCentral Disease Investigation Laboratory, Department of Livestock Services, Dhaka, BangladeshDepartment of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, BangladeshDepartment of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, BangladeshDepartment of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, BangladeshDepartment of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, BangladeshLivestock Research Institute, Department of Livestock Services, Dhaka, BangladeshDepartment of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, BangladeshDepartment of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, BangladeshBackgroundNewcastle disease virus (NDV) genotype VII severely affects poultry, causing respiratory and neurological symptoms with a high rate of morbidity and mortality. The research aimed to develop an inactivated ND vaccine using local isolates (Genotype VII.2) and assess its immunogenicity compared to other commercial live ND vaccines.MethodsAn inactivated vaccine using a candidate NDV (GenBank: OR924274.1) was developed according to WOAH guidelines following inactivation, sterility, purity, and safety tests. The birds were vaccinated through subcutaneous (SC) and intramuscular (IM) routes using three doses (0.25, 0.5, and 1.0 ml/bird). Immunogenicity and protective potentiality of the experimentally developed inactivated ND vaccine and live commercial ND vaccine (intra-ocularly/IO) were compared by challenge studies using three vaccination schedules: killed-followed-killed, live-followed-killed, and live-followed-live.ResultsThe birds vaccinated with 1.0 ml/bird SC showed higher antibody titers compared to those of IM-vaccinated groups. Birds vaccinated with the live-followed-killed commercial ND vaccines had slightly higher antibody titers compared to those vaccinated with killed-followed-killed and live-followed-live vaccines. Birds vaccinated with the killed-followed-killed ND vaccine showed a higher protection rate (100%) compared to live-followed-killed (83±5.77%) and live-followed-live (57±5.77%) vaccines. Birds vaccinated with killed-followed-killed group showed a slower decline rate of antibody titers than other groups. This regimen provided significantly better immunity, highlighting its potential in controlling ND outbreaks in Bangladesh's poultry.ConclusionThe study found that the inactivated ND vaccine, developed with the locally circulating isolate of genotype-VII.2 of NDV, might play an important role in effective control and management of ND in the commercial poultry population in Bangladesh.https://www.frontiersin.org/articles/10.3389/fimmu.2024.1482314/fullNDV genotype VII.2inactivated ND vaccineimmunogenicityprotective potentialityantibody titers |
| spellingShingle | Mohammad Aynul Haque Md. Enamul Haque Mst. Kohinoor Parvin Md. Mostofa Kamal Tanbin Rubaiya Islam Mohammad Sadekuzzaman Md. Ariful Islam Mst. Minara Khatun Muhammad Tofazzal Hossain Mohammad Asir Uddin Sham Soun Nahar A. K. M. Khasruzzaman Md. Alimul Islam Determination of immunogenicity of an inactivated ND-vaccine developed experimentally with Newcastle disease virus (Genotype VII.2) local isolates of Bangladesh Frontiers in Immunology NDV genotype VII.2 inactivated ND vaccine immunogenicity protective potentiality antibody titers |
| title | Determination of immunogenicity of an inactivated ND-vaccine developed experimentally with Newcastle disease virus (Genotype VII.2) local isolates of Bangladesh |
| title_full | Determination of immunogenicity of an inactivated ND-vaccine developed experimentally with Newcastle disease virus (Genotype VII.2) local isolates of Bangladesh |
| title_fullStr | Determination of immunogenicity of an inactivated ND-vaccine developed experimentally with Newcastle disease virus (Genotype VII.2) local isolates of Bangladesh |
| title_full_unstemmed | Determination of immunogenicity of an inactivated ND-vaccine developed experimentally with Newcastle disease virus (Genotype VII.2) local isolates of Bangladesh |
| title_short | Determination of immunogenicity of an inactivated ND-vaccine developed experimentally with Newcastle disease virus (Genotype VII.2) local isolates of Bangladesh |
| title_sort | determination of immunogenicity of an inactivated nd vaccine developed experimentally with newcastle disease virus genotype vii 2 local isolates of bangladesh |
| topic | NDV genotype VII.2 inactivated ND vaccine immunogenicity protective potentiality antibody titers |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2024.1482314/full |
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