The Gut Bacteria of <i>Gampsocleis gratiosa</i> (Orthoptera: Tettigoniidae) by Culturomics
<i>Gampsocleis gratiosa</i> Brunner von Wattenwyl, 1862, is a type of omnivorous chirping insect with a long history of artificial breeding. It has high economic value and is also an excellent orthopteran model organism. In this study, 12 types of culture media combined with 16S rRNA seq...
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2025-01-01
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| author | Hongmei Li Huimin Huang Ying Jia Yuwei Tong Zhijun Zhou |
| author_facet | Hongmei Li Huimin Huang Ying Jia Yuwei Tong Zhijun Zhou |
| author_sort | Hongmei Li |
| collection | DOAJ |
| description | <i>Gampsocleis gratiosa</i> Brunner von Wattenwyl, 1862, is a type of omnivorous chirping insect with a long history of artificial breeding. It has high economic value and is also an excellent orthopteran model organism. In this study, 12 types of culture media combined with 16S rRNA sequencing were employed to isolate 838 bacterial strains from the gut of <i>G. gratiosa</i>. After sequence comparison, a total of 98 species of bacteria were identified, belonging to 3 phyla, 5 classes, 11 orders, 20 families, and 45 genera. Firmicutes and Proteobacteria accounted for the majority (92.86%). At the order level, Enterobacteriaceae, Bacillales, and Lactobacillales predominated (79.59%). At the genus level, <i>Klebsiella</i> (11.22%) and <i>Enterococcus</i> (7.14%) predominated. This study also enumerated the strain morphological, physiological and biochemical properties of 98 species of bacteria, including colony morphology, Gram staining, bacterial motility test, temperature gradient growth, pH gradient growth, citrate utilization test, temperature oxidase test, contact enzyme test, methyl red test, V-P test, indole test, gelatin liquefaction test, nitrate reduction test, hydrogen sulfide test, starch hydrolysis test, cellulose decomposition test, esterase (corn oil) test and antibiotic susceptibility testing. Additionally, 16 antibiotics were utilized to test the bacterial susceptibility of the strains. This study explored the types and community structure of some culturable microorganisms in the intestinal tract of <i>G. gratiosa</i> and recorded their physiological characteristics. These data reflect the physiological functions of the intestinal microorganisms of <i>G. gratiosa</i> and provide support for subsequent research on the interaction mechanism between microorganisms and their hosts. |
| format | Article |
| id | doaj-art-8bf3a1e6b6fe4921a581d08ccfd96c3a |
| institution | DOAJ |
| issn | 2075-4450 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Insects |
| spelling | doaj-art-8bf3a1e6b6fe4921a581d08ccfd96c3a2025-08-20T02:45:00ZengMDPI AGInsects2075-44502025-01-0116212310.3390/insects16020123The Gut Bacteria of <i>Gampsocleis gratiosa</i> (Orthoptera: Tettigoniidae) by CulturomicsHongmei Li0Huimin Huang1Ying Jia2Yuwei Tong3Zhijun Zhou4Key Laboratory of Zoological Systematics and Application of Hebei Province, College of Life Sciences, Hebei University, Baoding 071002, ChinaKey Laboratory of Zoological Systematics and Application of Hebei Province, College of Life Sciences, Hebei University, Baoding 071002, ChinaKey Laboratory of Zoological Systematics and Application of Hebei Province, College of Life Sciences, Hebei University, Baoding 071002, ChinaKey Laboratory of Zoological Systematics and Application of Hebei Province, College of Life Sciences, Hebei University, Baoding 071002, ChinaKey Laboratory of Zoological Systematics and Application of Hebei Province, College of Life Sciences, Hebei University, Baoding 071002, China<i>Gampsocleis gratiosa</i> Brunner von Wattenwyl, 1862, is a type of omnivorous chirping insect with a long history of artificial breeding. It has high economic value and is also an excellent orthopteran model organism. In this study, 12 types of culture media combined with 16S rRNA sequencing were employed to isolate 838 bacterial strains from the gut of <i>G. gratiosa</i>. After sequence comparison, a total of 98 species of bacteria were identified, belonging to 3 phyla, 5 classes, 11 orders, 20 families, and 45 genera. Firmicutes and Proteobacteria accounted for the majority (92.86%). At the order level, Enterobacteriaceae, Bacillales, and Lactobacillales predominated (79.59%). At the genus level, <i>Klebsiella</i> (11.22%) and <i>Enterococcus</i> (7.14%) predominated. This study also enumerated the strain morphological, physiological and biochemical properties of 98 species of bacteria, including colony morphology, Gram staining, bacterial motility test, temperature gradient growth, pH gradient growth, citrate utilization test, temperature oxidase test, contact enzyme test, methyl red test, V-P test, indole test, gelatin liquefaction test, nitrate reduction test, hydrogen sulfide test, starch hydrolysis test, cellulose decomposition test, esterase (corn oil) test and antibiotic susceptibility testing. Additionally, 16 antibiotics were utilized to test the bacterial susceptibility of the strains. This study explored the types and community structure of some culturable microorganisms in the intestinal tract of <i>G. gratiosa</i> and recorded their physiological characteristics. These data reflect the physiological functions of the intestinal microorganisms of <i>G. gratiosa</i> and provide support for subsequent research on the interaction mechanism between microorganisms and their hosts.https://www.mdpi.com/2075-4450/16/2/123<i>Gampsocleis gratiosa</i>culturomicsgut bacteria |
| spellingShingle | Hongmei Li Huimin Huang Ying Jia Yuwei Tong Zhijun Zhou The Gut Bacteria of <i>Gampsocleis gratiosa</i> (Orthoptera: Tettigoniidae) by Culturomics Insects <i>Gampsocleis gratiosa</i> culturomics gut bacteria |
| title | The Gut Bacteria of <i>Gampsocleis gratiosa</i> (Orthoptera: Tettigoniidae) by Culturomics |
| title_full | The Gut Bacteria of <i>Gampsocleis gratiosa</i> (Orthoptera: Tettigoniidae) by Culturomics |
| title_fullStr | The Gut Bacteria of <i>Gampsocleis gratiosa</i> (Orthoptera: Tettigoniidae) by Culturomics |
| title_full_unstemmed | The Gut Bacteria of <i>Gampsocleis gratiosa</i> (Orthoptera: Tettigoniidae) by Culturomics |
| title_short | The Gut Bacteria of <i>Gampsocleis gratiosa</i> (Orthoptera: Tettigoniidae) by Culturomics |
| title_sort | gut bacteria of i gampsocleis gratiosa i orthoptera tettigoniidae by culturomics |
| topic | <i>Gampsocleis gratiosa</i> culturomics gut bacteria |
| url | https://www.mdpi.com/2075-4450/16/2/123 |
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