<i>RtHSFA9s</i> of <i>Rhodomyrtus tomentosa</i> Positively Regulate Thermotolerance by Transcriptionally Activating <i>RtHSFA2s</i> and <i>RtHSPs</i>
Heat shock transcription factors (HSFs) are crucial components in heat stress response. However, the contribution of the HSFs governing the inherent thermotolerance in <i>Rhodomyrtus tomentosa</i> has barely been investigated. We here compared the roles of <i>RtHSFA9a</i>, &l...
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2024-12-01
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| author | Huiguang Li Ling Yang Yujie Fang Gui Wang Tingting Liu |
| author_facet | Huiguang Li Ling Yang Yujie Fang Gui Wang Tingting Liu |
| author_sort | Huiguang Li |
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| description | Heat shock transcription factors (HSFs) are crucial components in heat stress response. However, the contribution of the HSFs governing the inherent thermotolerance in <i>Rhodomyrtus tomentosa</i> has barely been investigated. We here compared the roles of <i>RtHSFA9a</i>, <i>RtHSFA9b</i>, and <i>RtHSFA9c</i> in heat stress tolerance. These three genes are the results of gene duplication events, but there exist vast variations in their amino acid sequences. They are all localized to the nucleus. <i>Arabidopsis thaliana</i> plants with overexpressed <i>RtHSFA9a</i> and <i>RtHSFA9c</i> outperformed the wild-type plants, while the over-accumulation of <i>RtHSFA9b</i> had little impact on plant thermotolerance. By transiently overexpressing <i>RtHSFA9a</i>, <i>RtHSFA9b</i>, and <i>RtHSFA9c</i> in <i>R. tomentosa</i> seedlings, the mRNA abundance of heat shock response genes, including <i>RtHSFA2a</i>, <i>RtHSFA2b</i>, <i>RtHSP17.4</i>, <i>RtHSP21.8</i>, <i>RtHSP26.5</i>, and <i>RtHSP70</i>, were upregulated. Transactivation assays confirmed that there exist regulatory divergences among these three genes, viz., <i>RtHSFA9a</i> has the highest transcription activity in regulating <i>RtHSFA2a</i>, <i>RtHSFA2b</i>, <i>RtHSP21.8</i>, and <i>RtHSP70</i>; <i>RtHSFA9c</i> can transcriptionally activate <i>RtHSFA2b</i>, <i>RtHSP21.8</i>, and <i>RtHSP70</i>; <i>RtHSFA9b</i> makes limited contributions to the accumulation of <i>RtHSFA2b</i>, <i>RtHSP21.8</i>, and <i>RtHSP70</i>. Our results indicate that the <i>RtHSFA9</i> genes make crucial contributions to the thermal adaption of <i>R. tomentosa</i> by positively regulating the <i>RtHSFA2a</i>, <i>RtHSFA2b</i>, and <i>RtHSP</i> genes, which provides novel insights into the <i>RtHSFA9</i> subfamily. |
| format | Article |
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| spelling | doaj-art-8bebb681cc3e420e96dce7a66ce907e12025-08-20T02:53:44ZengMDPI AGLife2075-17292024-12-011412159110.3390/life14121591<i>RtHSFA9s</i> of <i>Rhodomyrtus tomentosa</i> Positively Regulate Thermotolerance by Transcriptionally Activating <i>RtHSFA2s</i> and <i>RtHSPs</i>Huiguang Li0Ling Yang1Yujie Fang2Gui Wang3Tingting Liu4Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement & Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, ChinaKey Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement & Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, ChinaCollege of Life Sciences, Gannan Normal University, Ganzhou 341000, ChinaKey Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement & Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, ChinaKey Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement & Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, ChinaHeat shock transcription factors (HSFs) are crucial components in heat stress response. However, the contribution of the HSFs governing the inherent thermotolerance in <i>Rhodomyrtus tomentosa</i> has barely been investigated. We here compared the roles of <i>RtHSFA9a</i>, <i>RtHSFA9b</i>, and <i>RtHSFA9c</i> in heat stress tolerance. These three genes are the results of gene duplication events, but there exist vast variations in their amino acid sequences. They are all localized to the nucleus. <i>Arabidopsis thaliana</i> plants with overexpressed <i>RtHSFA9a</i> and <i>RtHSFA9c</i> outperformed the wild-type plants, while the over-accumulation of <i>RtHSFA9b</i> had little impact on plant thermotolerance. By transiently overexpressing <i>RtHSFA9a</i>, <i>RtHSFA9b</i>, and <i>RtHSFA9c</i> in <i>R. tomentosa</i> seedlings, the mRNA abundance of heat shock response genes, including <i>RtHSFA2a</i>, <i>RtHSFA2b</i>, <i>RtHSP17.4</i>, <i>RtHSP21.8</i>, <i>RtHSP26.5</i>, and <i>RtHSP70</i>, were upregulated. Transactivation assays confirmed that there exist regulatory divergences among these three genes, viz., <i>RtHSFA9a</i> has the highest transcription activity in regulating <i>RtHSFA2a</i>, <i>RtHSFA2b</i>, <i>RtHSP21.8</i>, and <i>RtHSP70</i>; <i>RtHSFA9c</i> can transcriptionally activate <i>RtHSFA2b</i>, <i>RtHSP21.8</i>, and <i>RtHSP70</i>; <i>RtHSFA9b</i> makes limited contributions to the accumulation of <i>RtHSFA2b</i>, <i>RtHSP21.8</i>, and <i>RtHSP70</i>. Our results indicate that the <i>RtHSFA9</i> genes make crucial contributions to the thermal adaption of <i>R. tomentosa</i> by positively regulating the <i>RtHSFA2a</i>, <i>RtHSFA2b</i>, and <i>RtHSP</i> genes, which provides novel insights into the <i>RtHSFA9</i> subfamily.https://www.mdpi.com/2075-1729/14/12/1591heat shock transcription factorthermal adaptionfunctional divergencetransient expressiontransactivation |
| spellingShingle | Huiguang Li Ling Yang Yujie Fang Gui Wang Tingting Liu <i>RtHSFA9s</i> of <i>Rhodomyrtus tomentosa</i> Positively Regulate Thermotolerance by Transcriptionally Activating <i>RtHSFA2s</i> and <i>RtHSPs</i> Life heat shock transcription factor thermal adaption functional divergence transient expression transactivation |
| title | <i>RtHSFA9s</i> of <i>Rhodomyrtus tomentosa</i> Positively Regulate Thermotolerance by Transcriptionally Activating <i>RtHSFA2s</i> and <i>RtHSPs</i> |
| title_full | <i>RtHSFA9s</i> of <i>Rhodomyrtus tomentosa</i> Positively Regulate Thermotolerance by Transcriptionally Activating <i>RtHSFA2s</i> and <i>RtHSPs</i> |
| title_fullStr | <i>RtHSFA9s</i> of <i>Rhodomyrtus tomentosa</i> Positively Regulate Thermotolerance by Transcriptionally Activating <i>RtHSFA2s</i> and <i>RtHSPs</i> |
| title_full_unstemmed | <i>RtHSFA9s</i> of <i>Rhodomyrtus tomentosa</i> Positively Regulate Thermotolerance by Transcriptionally Activating <i>RtHSFA2s</i> and <i>RtHSPs</i> |
| title_short | <i>RtHSFA9s</i> of <i>Rhodomyrtus tomentosa</i> Positively Regulate Thermotolerance by Transcriptionally Activating <i>RtHSFA2s</i> and <i>RtHSPs</i> |
| title_sort | i rthsfa9s i of i rhodomyrtus tomentosa i positively regulate thermotolerance by transcriptionally activating i rthsfa2s i and i rthsps i |
| topic | heat shock transcription factor thermal adaption functional divergence transient expression transactivation |
| url | https://www.mdpi.com/2075-1729/14/12/1591 |
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