Genome-wide identification and expression profiling of the SWEET family in Actinidia polygama (Sieb. & Zucc.) Maxim.
Sugar was transported from photosynthetic source cells to sink cells, sugar efflux transporter protein (sugars will eventually be exported to transporters, SWEETs) play an important role in the process. Although SWEET family members had been identified in many plants, transcriptome or genomics analy...
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Maximum Academic Press
2024-01-01
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| Series: | Fruit Research |
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| Online Access: | https://www.maxapress.com/article/doi/10.48130/frures-0024-0010 |
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| author | Li Chen Hui-Fang Song Jia-Xin Liu Xu-Xin Jiang Jun Ai Zhen-Xing Wang Yun-Peng Wang |
| author_facet | Li Chen Hui-Fang Song Jia-Xin Liu Xu-Xin Jiang Jun Ai Zhen-Xing Wang Yun-Peng Wang |
| author_sort | Li Chen |
| collection | DOAJ |
| description | Sugar was transported from photosynthetic source cells to sink cells, sugar efflux transporter protein (sugars will eventually be exported to transporters, SWEETs) play an important role in the process. Although SWEET family members had been identified in many plants, transcriptome or genomics analysis of Actinidia polygama SWEET genes remains uncharacterized. In this study, 14 SWEET genes of Actinidia polygama were identified by protein Blast. The structural characteristics of SWEET genes showed that the number of amino acids encoded by the gene family was between 233 and 304, the relative molecular weight was between 25,918.83 and 33,192.12, the isoelectric point was within the range of 6.96 to 9.71, 14 ApSWEET from Actinidia polygama and the known grape and Arabidopsis SWEETs were divided into four clades (I, II, III, and IV) according to the phylogenetic relationships. The gene structure analysis showed that most of ApSWEET genes have six exons and five introns except ApSWEET5 and ApSWEET14. All ApSWEET proteins also contained P-loop, MtN3-slv, and transmembrane domain. Expression patterns of 14 ApSWEET in different organs and at different fruit developmental stages were analyzed. ApSWEET1 and ApSWEET5 exhibited tissue-specific expression, whereas other genes were more ubiquitously expressed. ApSWEET1, ApSWEET10, and ApSWEET11 exhibited higher expression in fruit. The results of this study provide insights into the characteristics of the SWEET genes in Actinidia polygama and may serve as a basis for further functional studies of such genes. |
| format | Article |
| id | doaj-art-8be007fbff5c48089cdf3ec8acdd76ea |
| institution | DOAJ |
| issn | 2769-4615 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Maximum Academic Press |
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| series | Fruit Research |
| spelling | doaj-art-8be007fbff5c48089cdf3ec8acdd76ea2025-08-20T03:18:54ZengMaximum Academic PressFruit Research2769-46152024-01-014111310.48130/frures-0024-0010frures-0024-0010Genome-wide identification and expression profiling of the SWEET family in Actinidia polygama (Sieb. & Zucc.) Maxim.Li Chen0Hui-Fang Song1Jia-Xin Liu2Xu-Xin Jiang3Jun Ai4Zhen-Xing Wang5Yun-Peng Wang6College of Horticulture, Jilin Agricultural University, Changchun, Jilin 130118, ChinaKey Laboratory of Forest Silviculture and Conservation of the Ministry of Education, College of Forestry, Beijing Forestry University, Beijing 100083, ChinaCollege of Horticulture, Jilin Agricultural University, Changchun, Jilin 130118, ChinaCollege of Horticulture, Jilin Agricultural University, Changchun, Jilin 130118, ChinaCollege of Horticulture, Jilin Agricultural University, Changchun, Jilin 130118, ChinaCollege of Horticulture, Jilin Agricultural University, Changchun, Jilin 130118, ChinaInstitute of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences, Changchun 130033, ChinaSugar was transported from photosynthetic source cells to sink cells, sugar efflux transporter protein (sugars will eventually be exported to transporters, SWEETs) play an important role in the process. Although SWEET family members had been identified in many plants, transcriptome or genomics analysis of Actinidia polygama SWEET genes remains uncharacterized. In this study, 14 SWEET genes of Actinidia polygama were identified by protein Blast. The structural characteristics of SWEET genes showed that the number of amino acids encoded by the gene family was between 233 and 304, the relative molecular weight was between 25,918.83 and 33,192.12, the isoelectric point was within the range of 6.96 to 9.71, 14 ApSWEET from Actinidia polygama and the known grape and Arabidopsis SWEETs were divided into four clades (I, II, III, and IV) according to the phylogenetic relationships. The gene structure analysis showed that most of ApSWEET genes have six exons and five introns except ApSWEET5 and ApSWEET14. All ApSWEET proteins also contained P-loop, MtN3-slv, and transmembrane domain. Expression patterns of 14 ApSWEET in different organs and at different fruit developmental stages were analyzed. ApSWEET1 and ApSWEET5 exhibited tissue-specific expression, whereas other genes were more ubiquitously expressed. ApSWEET1, ApSWEET10, and ApSWEET11 exhibited higher expression in fruit. The results of this study provide insights into the characteristics of the SWEET genes in Actinidia polygama and may serve as a basis for further functional studies of such genes.https://www.maxapress.com/article/doi/10.48130/frures-0024-0010actinidia polygamasweet gene familysugar transportgene expression analysis |
| spellingShingle | Li Chen Hui-Fang Song Jia-Xin Liu Xu-Xin Jiang Jun Ai Zhen-Xing Wang Yun-Peng Wang Genome-wide identification and expression profiling of the SWEET family in Actinidia polygama (Sieb. & Zucc.) Maxim. Fruit Research actinidia polygama sweet gene family sugar transport gene expression analysis |
| title | Genome-wide identification and expression profiling of the SWEET family in Actinidia polygama (Sieb. & Zucc.) Maxim. |
| title_full | Genome-wide identification and expression profiling of the SWEET family in Actinidia polygama (Sieb. & Zucc.) Maxim. |
| title_fullStr | Genome-wide identification and expression profiling of the SWEET family in Actinidia polygama (Sieb. & Zucc.) Maxim. |
| title_full_unstemmed | Genome-wide identification and expression profiling of the SWEET family in Actinidia polygama (Sieb. & Zucc.) Maxim. |
| title_short | Genome-wide identification and expression profiling of the SWEET family in Actinidia polygama (Sieb. & Zucc.) Maxim. |
| title_sort | genome wide identification and expression profiling of the sweet family in actinidia polygama sieb zucc maxim |
| topic | actinidia polygama sweet gene family sugar transport gene expression analysis |
| url | https://www.maxapress.com/article/doi/10.48130/frures-0024-0010 |
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