Complete blood count in neonatal Intensive care Unit (NICU): Performance comparison between POCT Sight OLO® and Sysmex XN-9100™ hematology analyzers
Background and aims: The use of a POCT (Point Of Care Test) could help in reducing the impact of pre-analytical errors in particular in challenging newborn samples.The study purpose is to compare the POCT Sight OLO® hematology analyzer, validated for >3 months patients, with the reference system...
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| Language: | English |
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Elsevier
2025-04-01
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| Series: | Practical Laboratory Medicine |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S235255172500006X |
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| author | Francesca Nencini Alessandro Bonari Sara Ciullini Mannurita Alessandra Mongia Francesca Romano Maria Garieri Edda Russo Silvia Sastrucci Graziella Marrani Martina Tonelli Stefano Salti Niccola Funel Amedeo Amedei Carlo Dani Alessandra Fanelli |
| author_facet | Francesca Nencini Alessandro Bonari Sara Ciullini Mannurita Alessandra Mongia Francesca Romano Maria Garieri Edda Russo Silvia Sastrucci Graziella Marrani Martina Tonelli Stefano Salti Niccola Funel Amedeo Amedei Carlo Dani Alessandra Fanelli |
| author_sort | Francesca Nencini |
| collection | DOAJ |
| description | Background and aims: The use of a POCT (Point Of Care Test) could help in reducing the impact of pre-analytical errors in particular in challenging newborn samples.The study purpose is to compare the POCT Sight OLO® hematology analyzer, validated for >3 months patients, with the reference system Sysmex XN-9100™ in Neonatal Intensive Care Unit (NICU). Material and methods: The two analyzers were compared through Passing-Bablok regression analysis and Bland-Altman plot. Results: We analyzed 65 blood samples, in detail 38 from adults and 27 from newborns.The regression analysis results performed in the newborn and adult patients showed a good agreement between the two instruments. The evaluation of the Bland-Altman plots showed comparable values of bias <10 % for the most of parameters.The evaluation of sample flags for the presence of distributional and morphological abnormalities showed a partial accordance between the two approaches, but the POCT exhibited good performance compared to the final report revised by the laboratory specialist. Conclusions: The comparison of the two instruments demonstrated that they provide comparable blood counts, also in patients aged <3 months. The POCT allows having reliable analytical data and faster turning around time, particularly useful in NICU. |
| format | Article |
| id | doaj-art-8bdbee8413dc47d08e48afa8788b2a6a |
| institution | DOAJ |
| issn | 2352-5517 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Practical Laboratory Medicine |
| spelling | doaj-art-8bdbee8413dc47d08e48afa8788b2a6a2025-08-20T02:50:48ZengElsevierPractical Laboratory Medicine2352-55172025-04-0144e0045310.1016/j.plabm.2025.e00453Complete blood count in neonatal Intensive care Unit (NICU): Performance comparison between POCT Sight OLO® and Sysmex XN-9100™ hematology analyzersFrancesca Nencini0Alessandro Bonari1Sara Ciullini Mannurita2Alessandra Mongia3Francesca Romano4Maria Garieri5Edda Russo6Silvia Sastrucci7Graziella Marrani8Martina Tonelli9Stefano Salti10Niccola Funel11Amedeo Amedei12Carlo Dani13Alessandra Fanelli14General Laboratory, Careggi University Hospital, Florence, ItalyGeneral Laboratory, Careggi University Hospital, Florence, ItalyGeneral Laboratory, Careggi University Hospital, Florence, ItalyGeneral Laboratory, Careggi University Hospital, Florence, ItalyGeneral Laboratory, Careggi University Hospital, Florence, ItalyDivision of Neonatology and Neonatal Intensive Care Unit, Careggi University Hospital, Florence, ItalyDepartment of Experimental and Clinical Medicine, University of Florence, ItalyGeneral Laboratory, Careggi University Hospital, Florence, ItalyGeneral Laboratory, Careggi University Hospital, Florence, ItalyGeneral Laboratory, Careggi University Hospital, Florence, ItalyGeneral Laboratory, Careggi University Hospital, Florence, ItalyChemical Chemistry Laboratory, Azienda Usl Toscana Nord Ovest, ItalyDepartment of Experimental and Clinical Medicine, University of Florence, ItalyDivision of Neonatology and Neonatal Intensive Care Unit, Careggi University Hospital, Florence, Italy; Corresponding author. University of Florence, Department of Neurosciences, Psychology, Drug Research and Child Health, University of Florence, Florence, Italy.General Laboratory, Careggi University Hospital, Florence, ItalyBackground and aims: The use of a POCT (Point Of Care Test) could help in reducing the impact of pre-analytical errors in particular in challenging newborn samples.The study purpose is to compare the POCT Sight OLO® hematology analyzer, validated for >3 months patients, with the reference system Sysmex XN-9100™ in Neonatal Intensive Care Unit (NICU). Material and methods: The two analyzers were compared through Passing-Bablok regression analysis and Bland-Altman plot. Results: We analyzed 65 blood samples, in detail 38 from adults and 27 from newborns.The regression analysis results performed in the newborn and adult patients showed a good agreement between the two instruments. The evaluation of the Bland-Altman plots showed comparable values of bias <10 % for the most of parameters.The evaluation of sample flags for the presence of distributional and morphological abnormalities showed a partial accordance between the two approaches, but the POCT exhibited good performance compared to the final report revised by the laboratory specialist. Conclusions: The comparison of the two instruments demonstrated that they provide comparable blood counts, also in patients aged <3 months. The POCT allows having reliable analytical data and faster turning around time, particularly useful in NICU.http://www.sciencedirect.com/science/article/pii/S235255172500006XComplete blood countHematology analyzerNewbornPerformance comparisonPOCT |
| spellingShingle | Francesca Nencini Alessandro Bonari Sara Ciullini Mannurita Alessandra Mongia Francesca Romano Maria Garieri Edda Russo Silvia Sastrucci Graziella Marrani Martina Tonelli Stefano Salti Niccola Funel Amedeo Amedei Carlo Dani Alessandra Fanelli Complete blood count in neonatal Intensive care Unit (NICU): Performance comparison between POCT Sight OLO® and Sysmex XN-9100™ hematology analyzers Practical Laboratory Medicine Complete blood count Hematology analyzer Newborn Performance comparison POCT |
| title | Complete blood count in neonatal Intensive care Unit (NICU): Performance comparison between POCT Sight OLO® and Sysmex XN-9100™ hematology analyzers |
| title_full | Complete blood count in neonatal Intensive care Unit (NICU): Performance comparison between POCT Sight OLO® and Sysmex XN-9100™ hematology analyzers |
| title_fullStr | Complete blood count in neonatal Intensive care Unit (NICU): Performance comparison between POCT Sight OLO® and Sysmex XN-9100™ hematology analyzers |
| title_full_unstemmed | Complete blood count in neonatal Intensive care Unit (NICU): Performance comparison between POCT Sight OLO® and Sysmex XN-9100™ hematology analyzers |
| title_short | Complete blood count in neonatal Intensive care Unit (NICU): Performance comparison between POCT Sight OLO® and Sysmex XN-9100™ hematology analyzers |
| title_sort | complete blood count in neonatal intensive care unit nicu performance comparison between poct sight olo r and sysmex xn 9100™ hematology analyzers |
| topic | Complete blood count Hematology analyzer Newborn Performance comparison POCT |
| url | http://www.sciencedirect.com/science/article/pii/S235255172500006X |
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