ELLIPSOMETRY, RAMAN SPECTROSCOPY AND SOI- NANOWIRE BIOSENSOR IN DIAGNOSIS OF COLORECTALCANCER
Differences in the rate constants of specific interactions between serum tumor M2-pyruvate kinase (Tumor M2-PK) and highly specific monoclonal antibodies deposited on the surface of biochips were found in colorectal cancer patients using surface plasmon resonance enhanced ellipsometry. Scanning elli...
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| Language: | Russian |
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Russian Academy of Sciences, Tomsk National Research Medical Center
2017-09-01
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| Series: | Сибирский онкологический журнал |
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| Online Access: | https://www.siboncoj.ru/jour/article/view/568 |
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| author | M. V. Kruchinina Ya. I. Prudnikova S. A. Kurilovich A. A. Gromov V. N. Kruchinin V. V. Atuchin O. V. Naumova E. V. Spesivtsev V. A. Volodin S. E. Peltek G. V. Shuvalov V. M. Generalov |
| author_facet | M. V. Kruchinina Ya. I. Prudnikova S. A. Kurilovich A. A. Gromov V. N. Kruchinin V. V. Atuchin O. V. Naumova E. V. Spesivtsev V. A. Volodin S. E. Peltek G. V. Shuvalov V. M. Generalov |
| author_sort | M. V. Kruchinina |
| collection | DOAJ |
| description | Differences in the rate constants of specific interactions between serum tumor M2-pyruvate kinase (Tumor M2-PK) and highly specific monoclonal antibodies deposited on the surface of biochips were found in colorectal cancer patients using surface plasmon resonance enhanced ellipsometry. Scanning ellipsometry revealed a significant increase in the biomolecular layer thickness caused by antigen-antibody reaction in patients with hepatic and extra-hepatic metastases compared to that in healthy subjects (p<0.001–0.042).The specificity of the interaction was confirmed by fluorescence optical spectrometry. The Raman spectra of serum samples revealed differences in the intensity of peaks appeared at 1005–1520 cm-1 in the same groups of patients (p<0.0001–0.05) with a predictive accuracy of 90 % for early-stage disease. The pilot experiments with a nanowire biosensor based on SOI (silicon on insulator), for example Tumor M2-PK, were carried out. High sensitivity (10-13–10-15M) and specificity in identifying antigens in serum samples of patients with colorectal cancer were demonstrated. The results obtained were useful for detecting early-stage disease, metastases and recurrence as well as for monitoring the quality of treatment in colorectal cancer patients. |
| format | Article |
| id | doaj-art-8bd755f18fc4497c8605a0135f340e4a |
| institution | Kabale University |
| issn | 1814-4861 2312-3168 |
| language | Russian |
| publishDate | 2017-09-01 |
| publisher | Russian Academy of Sciences, Tomsk National Research Medical Center |
| record_format | Article |
| series | Сибирский онкологический журнал |
| spelling | doaj-art-8bd755f18fc4497c8605a0135f340e4a2025-08-20T03:56:24ZrusRussian Academy of Sciences, Tomsk National Research Medical CenterСибирский онкологический журнал1814-48612312-31682017-09-01164324110.21294/1814-4861-2017-16-4-32-41466ELLIPSOMETRY, RAMAN SPECTROSCOPY AND SOI- NANOWIRE BIOSENSOR IN DIAGNOSIS OF COLORECTALCANCERM. V. Kruchinina0Ya. I. Prudnikova1S. A. Kurilovich2A. A. Gromov3V. N. Kruchinin4V. V. Atuchin5O. V. Naumova6E. V. Spesivtsev7V. A. Volodin8S. E. Peltek9G. V. Shuvalov10V. M. Generalov11Research Institute of Internal and Preventive Medicine ‑ Branch of the Institute of Cytology and Genetics; Siberian Scientific Research Institute for Metrology, ROSSTANDART.Research Institute of Internal and Preventive Medicine ‑ Branch of the Institute of Cytology and Genetics.Research Institute of Internal and Preventive Medicine ‑ Branch of the Institute of Cytology and Genetics.Research Institute of Internal and Preventive Medicine ‑ Branch of the Institute of Cytology and Genetics.Rzhanov Institute of Semiconductor Physics, SB RAS.Rzhanov Institute of Semiconductor Physics, SB RAS; Novosibirsk State University, Novosibirsk.Rzhanov Institute of Semiconductor Physics, SB RAS.Rzhanov Institute of Semiconductor Physics, SB RAS.Rzhanov Institute of Semiconductor Physics, SB RAS.Institute of Cytology and Genetics, SB RAS.Siberian Scientific Research Institute for Metrology, ROSSTANDART.State Research Center of Virology and Biotechnology «VECTOR».Differences in the rate constants of specific interactions between serum tumor M2-pyruvate kinase (Tumor M2-PK) and highly specific monoclonal antibodies deposited on the surface of biochips were found in colorectal cancer patients using surface plasmon resonance enhanced ellipsometry. Scanning ellipsometry revealed a significant increase in the biomolecular layer thickness caused by antigen-antibody reaction in patients with hepatic and extra-hepatic metastases compared to that in healthy subjects (p<0.001–0.042).The specificity of the interaction was confirmed by fluorescence optical spectrometry. The Raman spectra of serum samples revealed differences in the intensity of peaks appeared at 1005–1520 cm-1 in the same groups of patients (p<0.0001–0.05) with a predictive accuracy of 90 % for early-stage disease. The pilot experiments with a nanowire biosensor based on SOI (silicon on insulator), for example Tumor M2-PK, were carried out. High sensitivity (10-13–10-15M) and specificity in identifying antigens in serum samples of patients with colorectal cancer were demonstrated. The results obtained were useful for detecting early-stage disease, metastases and recurrence as well as for monitoring the quality of treatment in colorectal cancer patients.https://www.siboncoj.ru/jour/article/view/568diagnosiscolorectal cancerellipsometrysurface plasmon resonancefluorescence optical spectrometryraman spectroscopysoi nanowire biosensortumor m2 pyruvate kinase |
| spellingShingle | M. V. Kruchinina Ya. I. Prudnikova S. A. Kurilovich A. A. Gromov V. N. Kruchinin V. V. Atuchin O. V. Naumova E. V. Spesivtsev V. A. Volodin S. E. Peltek G. V. Shuvalov V. M. Generalov ELLIPSOMETRY, RAMAN SPECTROSCOPY AND SOI- NANOWIRE BIOSENSOR IN DIAGNOSIS OF COLORECTALCANCER Сибирский онкологический журнал diagnosis colorectal cancer ellipsometry surface plasmon resonance fluorescence optical spectrometry raman spectroscopy soi nanowire biosensor tumor m2 pyruvate kinase |
| title | ELLIPSOMETRY, RAMAN SPECTROSCOPY AND SOI- NANOWIRE BIOSENSOR IN DIAGNOSIS OF COLORECTALCANCER |
| title_full | ELLIPSOMETRY, RAMAN SPECTROSCOPY AND SOI- NANOWIRE BIOSENSOR IN DIAGNOSIS OF COLORECTALCANCER |
| title_fullStr | ELLIPSOMETRY, RAMAN SPECTROSCOPY AND SOI- NANOWIRE BIOSENSOR IN DIAGNOSIS OF COLORECTALCANCER |
| title_full_unstemmed | ELLIPSOMETRY, RAMAN SPECTROSCOPY AND SOI- NANOWIRE BIOSENSOR IN DIAGNOSIS OF COLORECTALCANCER |
| title_short | ELLIPSOMETRY, RAMAN SPECTROSCOPY AND SOI- NANOWIRE BIOSENSOR IN DIAGNOSIS OF COLORECTALCANCER |
| title_sort | ellipsometry raman spectroscopy and soi nanowire biosensor in diagnosis of colorectalcancer |
| topic | diagnosis colorectal cancer ellipsometry surface plasmon resonance fluorescence optical spectrometry raman spectroscopy soi nanowire biosensor tumor m2 pyruvate kinase |
| url | https://www.siboncoj.ru/jour/article/view/568 |
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