Comparison of cellular, mechanical, and optical properties of different polymers for corneal tissue engineering
Objective(s): The invention of corneal tissue engineering is essential for vision due to the lack of effective treatments and donated corneas. Finding the right polymer is crucial for reducing inflammation, ensuring biocompatibility, and mimicking natural cornea properties.Materials and Methods: In...
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| Format: | Article |
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Mashhad University of Medical Sciences
2025-08-01
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| Series: | Iranian Journal of Basic Medical Sciences |
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| Online Access: | https://ijbms.mums.ac.ir/article_26051_98286be114a1b43fd66cabc9003ce916.pdf |
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| author | Sepehr Zamani Sadeghi Javad Mohammad Kamalabadi-Farahani Seyede Nazanin Aghayan Zohreh Arabpour Ali Djalilian Majid Salehi |
| author_facet | Sepehr Zamani Sadeghi Javad Mohammad Kamalabadi-Farahani Seyede Nazanin Aghayan Zohreh Arabpour Ali Djalilian Majid Salehi |
| author_sort | Sepehr Zamani |
| collection | DOAJ |
| description | Objective(s): The invention of corneal tissue engineering is essential for vision due to the lack of effective treatments and donated corneas. Finding the right polymer is crucial for reducing inflammation, ensuring biocompatibility, and mimicking natural cornea properties.Materials and Methods: In this study, solvent casting and physical crosslinking (freeze-thaw cycles) were used to fabricate polymeric scaffolds of Polyvinyl alcohol, alginate, gelatin, carboxymethyl chitosan, carboxymethyl cellulose, polyacrylic acid, polyvinyl pyrrolidone, and their combinations. The mechanical evaluation of scaffolds for tension and suture ability was conducted. Biodegradability, swelling, water vapor, bacterial permeability, anti-inflammatory properties, blood compatibility, Blood Clotting Index (BCI), pH alterations, and cell compatibility with human Mesenchymal Stem cells (MSCs) were investigated with MTT. The hydrophilicity of the samples and the ability to adhere to surfaces were also compared with the contact angle and adhesive test, respectively. Finally, quantitative and qualitative analysis was used to check the transparency of the samples.Results: The mechanical strength of polyvinyl alcohol and polyvinyl pyrrolidone samples was highest, showing good suture ability. All samples had blood compatibility below 5% and cell compatibility above 75%. Polyvinyl alcohol was the most transparent at around 93%. Carboxymethyl chitosan effectively inhibited bacterial permeability, while its anti-inflammatory potential showed no significant difference.Conclusion: This study aims to choose the best polymer composition for corneal tissue engineering. The selection depends on the study’s goals, like mechanical strength or transparency. Comparing polymers across different dimensions provides better insight for polymer selection. |
| format | Article |
| id | doaj-art-8bd0b97ebf7b46839a3d0eea8e0c7c55 |
| institution | DOAJ |
| issn | 2008-3866 2008-3874 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj-art-8bd0b97ebf7b46839a3d0eea8e0c7c552025-08-20T02:48:09ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742025-08-012881082109910.22038/ijbms.2025.85468.1847726051Comparison of cellular, mechanical, and optical properties of different polymers for corneal tissue engineeringSepehr Zamani0Sadeghi Javad1Mohammad Kamalabadi-Farahani2Seyede Nazanin Aghayan3Zohreh Arabpour4Ali Djalilian5Majid Salehi6Student Research Committee, School of Medicine, Shahroud University of Medical Sciences, Shahroud, IranEye Research Center, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Tissue Engineering, School of Medicine, Shahroud University of Medical Sciences, Shahroud, IranStudent Research Committee, School of Medicine, Shahroud University of Medical Sciences, Shahroud, IranDepartment of Ophthalmology and Visual Sciences, University of Illinois at Chicago, IL, USADepartment of ophthalmology and Visual Sciences, University of Illinois at Chicago, IL, USADepartment of Tissue Engineering, School of Medicine, Shahroud University of Medical Sciences, Shahroud, IranObjective(s): The invention of corneal tissue engineering is essential for vision due to the lack of effective treatments and donated corneas. Finding the right polymer is crucial for reducing inflammation, ensuring biocompatibility, and mimicking natural cornea properties.Materials and Methods: In this study, solvent casting and physical crosslinking (freeze-thaw cycles) were used to fabricate polymeric scaffolds of Polyvinyl alcohol, alginate, gelatin, carboxymethyl chitosan, carboxymethyl cellulose, polyacrylic acid, polyvinyl pyrrolidone, and their combinations. The mechanical evaluation of scaffolds for tension and suture ability was conducted. Biodegradability, swelling, water vapor, bacterial permeability, anti-inflammatory properties, blood compatibility, Blood Clotting Index (BCI), pH alterations, and cell compatibility with human Mesenchymal Stem cells (MSCs) were investigated with MTT. The hydrophilicity of the samples and the ability to adhere to surfaces were also compared with the contact angle and adhesive test, respectively. Finally, quantitative and qualitative analysis was used to check the transparency of the samples.Results: The mechanical strength of polyvinyl alcohol and polyvinyl pyrrolidone samples was highest, showing good suture ability. All samples had blood compatibility below 5% and cell compatibility above 75%. Polyvinyl alcohol was the most transparent at around 93%. Carboxymethyl chitosan effectively inhibited bacterial permeability, while its anti-inflammatory potential showed no significant difference.Conclusion: This study aims to choose the best polymer composition for corneal tissue engineering. The selection depends on the study’s goals, like mechanical strength or transparency. Comparing polymers across different dimensions provides better insight for polymer selection.https://ijbms.mums.ac.ir/article_26051_98286be114a1b43fd66cabc9003ce916.pdfbiocompatible polymerscorneamesenchymal stem cellssolvent castingtransparent scaffolds |
| spellingShingle | Sepehr Zamani Sadeghi Javad Mohammad Kamalabadi-Farahani Seyede Nazanin Aghayan Zohreh Arabpour Ali Djalilian Majid Salehi Comparison of cellular, mechanical, and optical properties of different polymers for corneal tissue engineering Iranian Journal of Basic Medical Sciences biocompatible polymers cornea mesenchymal stem cells solvent casting transparent scaffolds |
| title | Comparison of cellular, mechanical, and optical properties of different polymers for corneal tissue engineering |
| title_full | Comparison of cellular, mechanical, and optical properties of different polymers for corneal tissue engineering |
| title_fullStr | Comparison of cellular, mechanical, and optical properties of different polymers for corneal tissue engineering |
| title_full_unstemmed | Comparison of cellular, mechanical, and optical properties of different polymers for corneal tissue engineering |
| title_short | Comparison of cellular, mechanical, and optical properties of different polymers for corneal tissue engineering |
| title_sort | comparison of cellular mechanical and optical properties of different polymers for corneal tissue engineering |
| topic | biocompatible polymers cornea mesenchymal stem cells solvent casting transparent scaffolds |
| url | https://ijbms.mums.ac.ir/article_26051_98286be114a1b43fd66cabc9003ce916.pdf |
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